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Four isolates of infectious pancreatic necrosis virus showed ringed plaque morphology on chinook salmon embryo cells, while six other isolates showed clear plaque morphology. One isolate chosen from the ringed-plaque class yielded defective interfering (DI) particles after as few as two virus passages starting with plaque-purified material; another isolate from the clear-plaque class failed to yield detectable numbers of DI particles.
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