1887

Abstract

SUMMARY

The course and outcome of intraperitoneally induced infections with the avirulent strain A7(74 ) of Semliki Forest virus have been studied in athymic ‘nude’ (nu/nu) mice, their heterozygous (nu/+) littermates and conventional Swiss AG mice. The main distinguishing characteristics of the infection in the nu/nu mice were the persistence of virus in the brain after an initial phase of incomplete virus clearance and the apparent establishment of a secondary phase of virus replication in the brain which was associated with a falling neutralizing antibody response. This secondary phase of virus replication persisted until at least the 28th day after inoculation. In addition the typical histological lesions of encephalitis induced by this virus were rare and focal demyelination, which occurred at a light microscopy level in up to 26 % of nu/+ and Swiss AG mice, was not observed. It is suggested that in immunocompetent mice the development of lesions including demyelination may be a result of an immunopathological response to virus infection which is related to the presence of thymus derived lymphocytes.

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/content/journal/jgv/10.1099/0022-1317-41-3-599
1978-12-01
2024-03-28
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References

  1. Bradish C. J., Allner K., Fitzgeorge R. 1975; Immunomodification and the expression of virulence in mice by defined strains of Semliki Forest virus: the effects of cyclophosphamide. Journal of General Virology 28:225–237
    [Google Scholar]
  2. Bradish C. J., Allner K., Maber H. B. 1971; The virulence of original and derived strains of Semliki Forest virus for mice guinea-pigs and rabbits. Journal of General Virology 12:141–160
    [Google Scholar]
  3. Burns W. H., Billups L. C., Noikins A. L. 1975; Thymus dependence of viral antigens. Nature, London 256:654–656
    [Google Scholar]
  4. Chew-Lim M. 1975; Mouse encephalitis induced by avirulent Semliki Forest virus. Veterinary Pathology 12:387–393
    [Google Scholar]
  5. Chew-Lim M., Suckling A. J., Webb H. E. 1977; Demyelination in mice after two or three infections with avirulent Semliki Forest virus. Veterinary Pathology 14:67–72
    [Google Scholar]
  6. Christoffersen P. J., Volkert M., Rygaard J. 1976; Immunological unresponsiveness of nude mice to LCM virus infection. Acta Pathologica et Microbiologica Scandinavica, section C 84:520–523
    [Google Scholar]
  7. Clarke D. H., Casals J. 1958; Techniques for haemagglutination and haemagglutination inhibition with arthropod borne viruses. American Journal of Tropical Medicine and Hygiene 7:561–573
    [Google Scholar]
  8. Cole G. A., Nathanson N., Prendergast R. A. 1972; Requirement for θ-bearing cells in lymphocytic choriomeningitis virus-induced central nervous system disease. Nature, London 238:335–337
    [Google Scholar]
  9. Flanagan S. P. 1966; ‘Nude’, a new hairless gene with pleiotropic effects in the mouse. Genetical Research, Cambridge 8:295–309
    [Google Scholar]
  10. Fleming P. 1977; Age-dependent and strain-related differences of virulence of Semliki Forest virus in mice. Journal of General Virology 37:93–105
    [Google Scholar]
  11. Levey R. H., Medawar P. B. 1966; The nature and mode of action of antilymphocyte serum. Proceedings of The National Academy of Sciences of the United States of America 56:1130–1137
    [Google Scholar]
  12. Mackenzie A., Suckling A. J., Jagelman S., Wilson A. M. 1978; Histopathological and enzyme histochemical changes in experimental Semliki Forest virus infection in mice and their relevance to scrapie. Journal of Comparative Pathology 88:335–344
    [Google Scholar]
  13. Mori R., Hino Y., Taniguchi T., Nomoto K., Takeya K. 1976; The ability of athymic nude mice to survive intracerebral infection of lymphocytic choriomeningitis (LCM) virus. Japanese Journal of Microbiology 20:249–250
    [Google Scholar]
  14. Pantelouris E. M. 1968; Absence of thymus in a mouse mutant. Nature, London 217:370–371
    [Google Scholar]
  15. Pritchard H., Micklem H. S. 1974; The nude (nu/nu) mouse as a model of thymus and T-lymphocyte deficiency. In Proceedings of The First International Workshop on Nude Mice127–139 Rygaard J., Povlsen C. O. Stuttgart: Gustav Fischer Verlag;
    [Google Scholar]
  16. Reed L. J., Muench H. 1938; A simple method of estimating fifty per cent end points. American Journal of Hygiene 27:493–497
    [Google Scholar]
  17. Sever J. L. 1962; Application of a microtechnique to viral serological investigations. Journal of Immunology 88:320–329
    [Google Scholar]
  18. Suckling A. J., Jagelman S., Webb H. E. 1977; Brain lysosomal glycosidase activity in immunosuppressed mice infected with avirulent Semliki Forest virus. Infection and Immunity 15:386–391
    [Google Scholar]
  19. Suckling A. J., Pathak S., Jagelman S., Webb H. E. 1978; Virus associated demyelination: a model using avirulent Semliki Forest virus infection of mice. Journal of the Neurological Sciences 36: in the press
    [Google Scholar]
  20. Turk J. L. 1972; The use of cyclophosphamide in non-neoplastic disease. Proceedings of the Royal Society of Medicine 66:805–807
    [Google Scholar]
  21. Warren J., Weil M. L., Russ S. B., Jefferies H. 1949; Purification of certain viruses by use of protamine sulphate. Proceedings of the Society for Experimental Biology and Medicine 72:662–664
    [Google Scholar]
  22. Wortis H. H. 1971; Immunological responses of ‘nude’ mice. Clinical and Experimental Immunology 8:305–317
    [Google Scholar]
  23. Zlotnik I., Grant D. P., Batter-Hatton D. 1972; Encephalopathy in mice following inapparent Semliki Forest virus (S.F.V.) infection. British Journal of Experimental Pathology 53:125–129
    [Google Scholar]
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