1887

Abstract

SUMMARY

Large and small plaque variants of A foot-and-mouth disease virus were shown to have specific antigenic determinants. Large plaque virus antigenic specificity was destroyed by trypsin treatment, but the small plaque antigen was resistant despite cleavage of the trypsin-sensitive polypeptide. The cleavage of polypeptide VP by trypsin resulted in the formation of a new antigen not present on untreated virus. The effects of chymotrypsin and trypsin on the polypeptides of the plaque variants have been examined and related to changes in antigenicity, infectivity, and exposure of the polypeptides at the surface of the capsid. The results are discussed in relation to the orientation of the trypsin-sensitive polypeptide in the virus capsid.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-41-3-549
1978-12-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/41/3/JV0410030549.html?itemId=/content/journal/jgv/10.1099/0022-1317-41-3-549&mimeType=html&fmt=ahah

References

  1. Bachrach H. L., Callis J. J., Hess W. R., Patty R. E., De Boer C. J., Hamblet F. E. 1962; Production of bovine kidney cultures for plaque assay of foot-and-mouth disease virus and its RNA. American Journal of Veterinary Research 23:608–613
    [Google Scholar]
  2. Bachrach H. L., Trautman R., Breese S. S. Jun. 1964; Chemical and physical properties of virtually pure foot-and-mouth disease virus. American Journal of Veterinary Research 25:333–342
    [Google Scholar]
  3. Bachrach H. L., Swaney J. B., Vande Woude G. F. 1973; Isolation of the structural polypeptides of foot-and-mouth disease virus and analysis of their C-terminal sequences. Virology 52:520–528
    [Google Scholar]
  4. Bachrach H. L., Moore D. M., Mckercher P. D., Polatnick J. 1975; Immune and antibody responses to an isolated capsid protein of foot-and-mouth disease virus. Journal of Immunology 115:1636–1641
    [Google Scholar]
  5. Brown F., Smale C. J. 1970; Demonstration of three specific sites on the surface of foot-and-mouth disease virus by antibody complexing. Journal of General Virology 7:115–127
    [Google Scholar]
  6. Cavanagh D., Sangar D. V., Rowlands D. J., Brown F. 1977; Immunogenic and cell attachment sites of FMDV: further evidence for their location in a single capsid polypeptide. Journal of General Virology 35:149–158
    [Google Scholar]
  7. Cowan K. M. 1968; Immunochemical studies of foot-and-mouth disease. IV. Preparation and evaluation of antisera specific for virus, virus protein subunit and the infection-associated antigen. Journal of Immunology 101:1183–1191
    [Google Scholar]
  8. Cowan K. M. 1969; Immunochemical studies of foot-and-mouth disease. V. Antigenic variants of virus demonstrated by immunodiffusion analyses with 19S but not 7S antibodies. Journal of Experimental Medicine 129:333–350
    [Google Scholar]
  9. Cowan K. M., Erol N., Whiteland A. P. 1974; Heterogeneity of type Asia 1 foot-and-mouth disease virus and BHK-21 cells and the relationship to vaccine preparation. Bulletin Office International Epizootics 81:1271–1298
    [Google Scholar]
  10. Cowan K. M., Erol N., Whiteland A. P. 1978; Effect of trypsin treatment on the antigenic characteristics of plaque variants of type O1 and type Asia-1 foot-and-mouth disease viruses. Journal of General Virology 41:437–442
    [Google Scholar]
  11. Cowan K. M., Graves J. H. 1966; A third antigenic component associated with foot-and-mouth disease infection. Virology 30:528–540
    [Google Scholar]
  12. Frenkel H. S. 1950; Research on foot-and-mouth disease. II. The cultivation of virus in explantations of tongue epithelium of bovine animals. American Journal of Veterinary Research 11:371–372
    [Google Scholar]
  13. Hummel B. C. W. 1959; A modified spectrophotometric determination of chymotrypsin and thrombin. Canadian Journal of Biology and Physiology 37:1393
    [Google Scholar]
  14. Hunter W. M., Greenwood F. C. 1962; Preparation of 131I-labelled human growth hormone of high specific activity. Nature, London 194:495–496
    [Google Scholar]
  15. Kaaden O. R., Adam K.-H., Strohmaier K. 1977; Induction of neutralizing antibodies and immunity in vaccinated guinea pigs by cyanogen bromide-peptides of VP3 of foot-and-mouth disease virus. Journal of General Virology 34:397–400
    [Google Scholar]
  16. Laporte J., Grosclaude J., Wantyghem J., Bernard S., Rouze P. 1973; Neutralisation en culture cellulaire du pouvoir infectieux du virus de la fìèvre aphteuse par des sérums provenant de pores immunises à l’aide d’une proteine virale purifiée. Comptes rendus hebdomadaires des séances de l’académie des sciences, Serie D, Sciences Naturelles France 276:3399–3401
    [Google Scholar]
  17. Laporte J., Lenoir G. 1973; Structural proteins of foot-and-mouth disease virus. Journal of General Virology 20:161–168
    [Google Scholar]
  18. Martinsen J. S. 1970; The effect of diethylaminoethyl dextran and agar overlay pH on plaque formation by two plaque size variants of foot-and-mouth disease virus. Canadian Journal of Comparative Medicine 34:13–19
    [Google Scholar]
  19. Mcvicar J. W., Sutmoller P. 1972a; Three variants of foot-and-mouth disease virus type O: Agar gel diffusion. American Journal of Veterinary Research 33:1635–1639
    [Google Scholar]
  20. Mcvicar J. W., Sutmoller P. 1972b; Three variants of foot-and-mouth disease virus type O: Exposure of cattle. American Journal of Veterinary Research 33:1641–1647
    [Google Scholar]
  21. Meloen R. H. 1976; Localisation on foot-and-mouth disease virus (FMDV) of an antigenic deficiency induced by passage in BHK cells. Archives of Virology 51:299–306
    [Google Scholar]
  22. Moore D. M. 1977; Characterization of three antigenic particles of swine vesicular disease virus. Journal of General Virology 34:431–445
    [Google Scholar]
  23. Polatnick J., Bachrach H. L. 1964; Production and purification of milligram amounts of foot-and-mouth disease virus from baby hamster kidney cell cultures. Applied Microbiology 12:368–373
    [Google Scholar]
  24. Rowlands D. J., Sangar D. V., Brown F. 1971; Relationship of the antigenic structure of foot-and-mouth disease virus to the process of infection. Journal of General Virology 13:85–93
    [Google Scholar]
  25. Strobbe R., Charlier G., Van Aert A., Debecq J., Leunen J. 1974; Polyacrylamide gel electrophoresis and immunogenicity of foot-and-mouth disease virus. Archiv fur experìmentelle Veterinàrmedizin 28:401–407
    [Google Scholar]
  26. Sutmoller P., Cowan K. M. 1974; The detection of foot-and-mouth disease virus antigens in infected cell cultures by immunoperoxidase techniques. Journal of General Virology 23:287–291
    [Google Scholar]
  27. Vamde Woude G. P., Swaney J. B., Bachrach H. L. 1972; Chemical and physical properties of foot-and-mouth disease virus: a comparison with Maus Elberfeld virus. Biochemical and Biophysical Research Communications 48:1222–1229
    [Google Scholar]
  28. Wagner G. G., Card J. L., Cowan K. M. 1970; Immunochemical studies of foot-and-mouth disease. VII. Characterization of foot-and-mouth disease virus concentrated by polyethylene glycol precipitation. Archiv für die gesamte Virusforschung 30:343–352
    [Google Scholar]
  29. Wild T. F., Brown P. 1967; Nature of the inactivating action of trypsin on foot-and-mouth disease virus. Journal of General Virology 1:247–250
    [Google Scholar]
  30. Wild T. F., Burroughs J. N., Brown F. 1969; Surface structure of foot-and-mouth disease virus. Journal of General Virology 4:313–320
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-41-3-549
Loading
/content/journal/jgv/10.1099/0022-1317-41-3-549
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error