Egg-grown mumps virus was used for purification of the glycoproteins of the virus. The two glycoproteins were separated by filtration on DEAE-Bio-Gel A columns and were used for preparation of rabbit hyperimmune sera. Antibodies directed against the large (mol. wt. 75 × 103) and small (mol. wt. 61 × 103) glycoprotein were immunologically distinct. Antiserum directed against the large glycoprotein inhibited haemagglutination, neuraminidase activity and virus infectivity, whereas antiserum against the small glycoprotein inhibited haemolysis, but not haemagglutination, neuraminidase activity or virus infectivity. It is concluded that mumps virus contains two glycoproteins on the envelope which are equivalent to the HN and F glycoproteins described for other paramyxoviruses.
Antibody activities of rabbit hyperimmune sera against untreated, Tween 80-ether and formalin treated purified virions were compared with those of sera against purified glycoproteins. In analogy with results obtained in studies on Sendai virus, these sera contained antibodies against both glycoprotein structures, but antibodies against the small glycoprotein blocking haemolysis could not be demonstrated.
The effect of formalin treatment on the two glycoprotein structures was studied in mixed haemadsorption experiments. Formalin treatment of mumps virus infected Vero cells resulted in destruction of the surface antigen of the small glycoprotein, whereas the surface antigen of the large glycoprotein was only moderately affected.
CarterC. H.,
ConwayT. J.,
CornfeldD.,
IezzoniD. G.,
KempeC. H.,
MoscoviciC.,
RatjhL. W.,
VignecA. J.,
WarrenJ.1962; Serologie responses of children to inactivated measles vaccine. Journal of the American Medical Association 179:848–853
EspmarkJ. A.,
FagraeusA.1965; Identification of the species of origin of cells by mixed hemadsorption - a mixed antiglobulin reaction applied to monolayer cultures. Journal of Immunology 94:530–537
HenleG.,
BasheW. J.,
BurgoonJ. S.,
BurgoonC. F.,
HuntG. R.,
HenleW.1951; Studies ΟΠ the prevention of mumps. III. The effect cf subcutaneous injection of inactivated mumps virus vaccines. Journal of Immunology 66:561–577
JohnT. J.,
FulginitiV. A.1966; Parainfluenza 2 virus: increase in hemagglutinin titer on treatment with Tween-80 and ether. Proceedings of the Society for Experimental Biology and Medicine 121:109–111
NorrbyE.1962; Hemagglutination by measles virus. 4. A simple procedure for production of high potency antigen for hemagglutination-inhibition (HI) tests. Proceedings of the Society for Experimental Biology and Medicine 111:814–818
NorrbyE.,
GollmarY.1972; Appearance and persistence of antibodies against different virus components after regular measles infections. Infection and Immunity 6:240–247
NorrbyE.,
Enders-RuckleG.,
Ter MeulenV.1975; Differences in the appearance of antibodies to structural components of measles virus after immunization with inactivated and live virus. Journal of Infectious Diseases 132:262–269
NorrbyE.1975; Occurrence of antibodies against envelope components after immunization with formalin inactivated and live measles vaccine. Journal of Biological Standardization 3:375–380
NorrbyE.,
LagercrantzR.1976; Measles vaccination. VIII. The occurrence of antibodies against virus envelope components after immunization with inactivated vaccine. Effects of revaccination with live measles vaccine. Acta paediatrica scandinavica 65:171–176
NorrbyE.,
GrandienM.,
ÖrvellC.1977; New tests for characterizations of mumps virus antibodies: hemolysis-inhibition, single radial immunodiffusion with immobilized virions, and mixed hemadsorption. Journal of Clinical Microbiology 5:346–352
ÖrvellC.1976; Identification of paramyxovirus-specific haemolysis-inhibiting antibodies separate from haemagglutinating-inhibiting and neuraminidase-inhibiting antibodies. Acta pathologica et microbiologica scandinavica section B 84:441–457
SalmiA. A.1969; Gel precipitation reactions between alkaline extracted rubella antigens and human sera. Acta pathologica et microbiologica scandinavica 76:271–278
SatoS.1958; Studies on hemagglutinating virus of Japan (HVJ) 10th report on the hemoiytic characteristics of HVJ (Sendai virus) in vitro
. Virus Kyoto 8:48–58
WigleyF. M.,
FruchtmanM. H.,
WaldmanR. H.1970; Aerosol immunization of humans with inactivated parainfluenza type 2 vaccine. New England Journal of Medicine 283:1250–1253