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1887

Abstract

SUMMARY

The 20 to 25 nm particles of hepatitis B surface antigen were purified from the serum of a carrier chimpanzee. Five major polypeptide species were revealed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Treatment of the particles with neuraminidase (EC 3.2.1.18) and galactose oxidase (EC 1.1.3.9) followed by reduction with tritiated sodium borohydride labelled galactose residues in a single glycoprotein with an apparent mol. wt. of 28000. The glycoprotein was not labelled when neuraminidase treatment was omitted, indicating that the galactose residues are in subterminal positions in the oligosaccharide chains. There was no significant incorporation of radiolabel into lipid. The serological activity of the antigen, as measured by a competitive double-antibody radioimmunoprecipitation assay, was not altered by the labelling procedure nor by exposure to neuraminidase alone.

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/content/journal/jgv/10.1099/0022-1317-41-3-447
1978-12-01
2025-01-17
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References

  1. Almeida J. D., Rubenstein D., Stott E. J. 1971; New antigen-antibody system in Australia-antigen positive hepatitis. Lancet ii:1225–1227
    [Google Scholar]
  2. Atassi M. Z. 1967; Specific cleavage of tryptophyl peptide bonds with periodate in sperm whale myoglobin. Archives of Biochemistry and Biophysics 120:56–59
    [Google Scholar]
  3. Bauer H. 1974; Virion and tumour cell antigens of C-type RNA tumour viruses. Advances in Cancer Research 20:275–341
    [Google Scholar]
  4. Bolognesi D. P., Collins J. J., Leis J. P., Moennig V., Schafer W., Atkinson P. H. 1975; Role of carbohydrate in determining the immunochemical properties of the major glycoprotein (gp 71) of Friend murine leukemia virus. Journal of Virology 16:1453–1463
    [Google Scholar]
  5. Bolton A. E., Hunter W. M. 1973; The labelling of proteins to high specific radioactivities by conjugation to a 125l-containing acylating agent. Biochemical Journal 133:529–539
    [Google Scholar]
  6. Burrell C. J., Proudvoot E., Keen G. A., Marmion B. P. 1973; Carbohydrates in hepatitis B antigen. Nature New Biology 243:261–262
    [Google Scholar]
  7. Burrell C. J., Leadbetter G., Mackay P., Marmion B. P. 1976; Tryptic cleavage of antibody binding sites from hepatitis B surface antigen particles. Journal of General Virology 33:41–5
    [Google Scholar]
  8. Cawley L. P. 1972; Reaction between concanavalin A and Australia antigen. American Journal of Clinical Pathology 57:253
    [Google Scholar]
  9. Chairez R., Steiner S., Melnick J. L., Dreesman G. R. 1973; Glycoproteins associated with hepatitis B antigen. Intervirology 1:224–228
    [Google Scholar]
  10. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  11. Etchison J. R., Holland J. J. 1974; Carbohydrate composition of the membrane glycoprotein of vesicular stomatitis virus grown in four mammalian cell lines. Proceedings of the National Academy of Sciences of the United States of America 71:4011–4014
    [Google Scholar]
  12. Etchison J. R., Robertson J. S., Summers D. F. 1977; Partial structural analysis of the oligosaccharide moieties of the vesicular stomatitis virus glycoprotein by sequential chemical and enzymatic degradation. Virology 78:375–392
    [Google Scholar]
  13. Gahmberg C. G. 1976; External labelling of human erythrocyte glycoproteins: studies with galactose oxidase and fluorography. Journal of Biological Chemistry 251:510–515
    [Google Scholar]
  14. Gerin J. L., Ford E. C., Purcell R. H. 1975; Biochemical characterization of Australia antigen. American Journal of Pathology 81:651–668
    [Google Scholar]
  15. Glossmann H., Neville D. M. Jun 1971; Glycoproteins of cell surfaces. A comparative study of three different cell surfaces of the rat. Journal of Biological Chemistry 246:6339–6346
    [Google Scholar]
  16. Howard C. R., Burrell C. J. 1976; Structure and nature of hepatitis B antigen. Progress in Medical Virology 22:36–103
    [Google Scholar]
  17. Johnson I., Clamp J. R. 1971; The oligosaccharide units of a human type L immunoglobulin (macro-globulin). Biochemical Journal 123:739–745
    [Google Scholar]
  18. Keegstra K., Sefton B. M., Burke D. 1975; Sindbis virus glycoproteins: effect of the host cell on the oligosaccharides. Journal of Virology 16:613–620
    [Google Scholar]
  19. Kelley J. M., Emerson S. U., Wagner R. R. 1972; The glycoprotein of vesicular stomatitis virus is the antigen that gives rise to, and reacts with, neutralizing antibody. Journal of Virology 10:1231–1235
    [Google Scholar]
  20. Krugman S., Giles J. P., Hammond J. 1971; Viral hepatitis, type B (MS-2 strain). Studies on active immunization. Journal of the American Medical Association 217:41–45
    [Google Scholar]
  21. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  22. Luukkonen A., Gahmberg C. G., Renkonen O. 1977; Surface labelling of SFV glycoproteins using galactose oxidase. Virology 76:55–59
    [Google Scholar]
  23. Mattila K., Luukkonen A., Renkonen O. 1976; Protein-bound oligosaccharides of Semliki Forest virus. Biochimica et Biophysica Acta 419:435–444
    [Google Scholar]
  24. Mclellan W. L., August J. T. 1976; Analysis of the envelope of Rauscher murine oncornavirus: in vitro labelling of glycopeptides. Journal of Virology 20:627–636
    [Google Scholar]
  25. Neurath A. R., Hashimoto N., Prince A. M. 1975; Sialyl residues in hepatitis B antigen: their role in determining the lifespan of the antigen in serum and in eliciting an immunological response. Journal of General Virology 27:81–91
    [Google Scholar]
  26. Peterson D. L., Roberts I. M., Vyas G. N. 1977; Partial amino acid sequence of two major component polypeptides of hepatitis B surface antigen. Proceedings of the National Academy of Science of the United States of America 74:1530–1534
    [Google Scholar]
  27. Prince A. M., Szmuness W., Woods K. R., Grady G. F. 1971; Antibody against serum-hepatitis antigen: prevalence and potential use to prevent hepatitis. New England Journal of Medicine 285:933–938
    [Google Scholar]
  28. Robinson W. S. 1974; A human hepatitis B virus candidate. In Mechanisms of Virus Disease225–241 Robinson W. S., Fox C. F. Menlo Park, California: Benjamin; Schulman J. L. 1975; Immunology of influenza. In The Influenza Viruses and Influenza chapter 12 373–393 Kilbourne E. D. New York, London and San Francisco: Academic Press;
    [Google Scholar]
  29. Sefton B. M. 1976; Virus-dependent glycosylation. Journal of Virology 17:85–93
    [Google Scholar]
  30. Sefton B. M., Keegstra K. 1974; Glycoproteins of Sindbis virus: preliminary characterisation of the oligosaccharides. Journal of Virology 14:522–530
    [Google Scholar]
  31. Shih J. W. K., Gerin J. L. 1977; Proteins of hepatitis B surface antigen. Journal of Virology 21:347–357
    [Google Scholar]
  32. Shiraishi H., Kohama T., Shirachi R., Ishida N. 1977; Carbohydrate composition of hepatitis B surface antigen. Journal of General Virology 36:207–210
    [Google Scholar]
  33. Shiraishi H., Shirachi R., Ishida N., Sekine T. 1978; The specific reduction of a and d antigenic determinants after periodate treatment of hepatitis B surface antigen (HBsAg). Journal of General Virology 38:363–367
    [Google Scholar]
  34. Spiro R. G. 1966; Analysis of sugars found in glycoproteins. In Methods of Enzymology 8 chapter I 3–26 Elizabeth F. Neufeld, Ginsburg Victor. New York and London: Academic Press;
    [Google Scholar]
  35. Steiner S., Huebner M. T., Dreesman G. R. 1974; Major polar lipids of hepatitis B antigen preparations: evidence for the presence of a glycosphingolipid. Journal of Virology 14:572–577
    [Google Scholar]
  36. Svennerholm L. 1957; Quantitative estimation of sialic acids, II. A colorimetric resorcinol-hydrochloric acid method. Biochimica et Biophysica Acta 24:604–611
    [Google Scholar]
  37. Trevelyan W. E., Procter D. P., Harrison J. S. 1950; Detection of sugars on paper chromatograms. Nature, London 166:444–445
    [Google Scholar]
  38. Weber K., Osborn M. 1969; The reliability of molecular weight determinations by dodecyl sulphate- polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
  39. world health organisation 1976 Technical Report Series No. 602. Memorandum – Viral Hepatitis.
    [Google Scholar]
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