The effect of interferon on the exponential growth phase of BALB/c 3T3 cells was studied. Although interferon reduced the growth rate and the proportion of cells in both the M and S phases (mitotic index and labelling index), there were no appreciable differences in the duration of these phases between control and interferon-treated cells. Moreover, the shape of the first peak of the fraction of labelled mitoses (FLM) curve was not altered by treatment with interferon, which indicates that the duration of the S and G2 phases was not affected. However, the height of the second peak of the curve in interferon-treated cells was extremely reduced as compared to control culture. These results are compatible with the idea that the suppressive effect of interferon is exerted mainly in the G1 phase (A-state) of the cell cycle of BALB/c 3T3 cells.
Collyn D’hoogheM.,
Brouty-BoyéD.,
MalaiseE. P.,
GresserI.1977; Interferon and cell division. XII. Prolongation by interferon of the intermitotic time of mouse mammary tumour cells in vitro. Microcinematographic analysis. Experimental Cell Research 105:73–77
KillanderD.,
LindahlP.,
LundinL.,
LearyP.,
GresserI.1976; Relationship between the enhanced expression of histocompatibility antigens on interferon-treated L1210 cells and their position in the cell cycle. European Journal of Immunology 6:56–59
KnightE.Jun1976; Antiviral and cell growth inhibitory activities reside in the same glycoprotein of human fibroblast interferon. Nature, London 262:302–303
Macieira-CoelhoA.,
Brouty-BoyéD.,
ThomasM. T.,
GresserI.1971; Interferon and cell division: III. Effect of interferon on the division cycle of L 1210 cells in vitro. Journal of Cell Biology 48:415–419
MatareseG. P.,
RossiG. B.1977; Effect of interferon on growth and division cycle of Friend erythroleukemic murine cells in vitro. Journal of Cell Biology 75:344–354
SokawaY.,
WatanabeY.,
WatanabeY.,
KawadeY.1977; Interferon suppresses the transition of quiescent 3T3 cells to a growing state. Nature, London 268:236–238
StewartW. E.II,
GresserI.,
ToveyM. G.,
BanduM.,
GoffS. L.1976; Identification of the cell multiplication inhibitory factors in interferon preparations as interferon. Nature, London 262:300–302
ToveyM.,
Brouty-BoyéD.,
GresserI.1975; Early effect of interferon on mouse leukemia cells cultivated in a chemostat. Proceedings of the National Academy of Sciences of the United States of America 72:2265–2269
YamamotoY.,
TsukuiK.,
OhwakiM.,
KawadeY.1974; Electrophoretic characterization of purified mouse L cell interferon of high specific activity. Journal of General Virology 23:23–32