1887

Abstract

SUMMARY

Temperature-sensitive mutants of the related orbiviruses, Wallal and Mudjinbarry, recombine with high frequency when grown in pairs in cell culture. The genome of each virus consists of discrete segments of double-stranded RNA and high frequency recombination suggests that reassortment of genome segments occurs rather than classical recombination. Electrophoresis in acrylamide gels of RNA extracted from the progeny of a cross between Wallal 101 and Mudjinbarry 3 mutants, revealed that three plaque isolates of 60 tested differed in RNA pattern from each of the parent viruses and from each other. Further analysis of the electrophoretic profiles suggested that the isolates were recombinants with RNA segments derived from each of the parent viruses.

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/content/journal/jgv/10.1099/0022-1317-41-2-333
1978-11-01
2022-01-24
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References

  1. Avital S., Elson D. 1969; A convenient method for preparing transfer RNA from Escherichia coli. Biochimica et Biophysica Acta 179 297 307
    [Google Scholar]
  2. Doherty R. L., Carley J. G., Standfast H. A., Dyce A. L., Kay B. H., Snowdon W. A. 1973; Isolation of arboviruses from mosquitoes, biting midges, sandflies and vertebrates collected in Queensland, 1969 and 1970. Transactions of the Royal Society of Tropical Medicine and Hygiene 67 536 543
    [Google Scholar]
  3. Doherty R. L., Standfast H. A., Dyce A. L., Carley J. G., Gorman B. M., Filippich C., Kay B. H. 1977; Mudjinbarry virus, an orbivirus of the Wallal group isolated from midges from the Northern Territory of Australia. Australian Journal of Biological Sciences 31 97 103
    [Google Scholar]
  4. Franklin R. M. 1966; Purification and properties of the replicative intermediate of the RNA bacteriophage R 17. Proceedings of the National Academy of Sciences of the United States of America 55 1504 1511
    [Google Scholar]
  5. Fields B. N. 1971; Temperature-sensitive mutants of reovirus type 3. Features of genetic recombination. Virology 46 142 148
    [Google Scholar]
  6. Gorman B. M., Lecatsas G. 1972; Formation of Wallal virus in cell culture. Onderstepoort Journal of Veterinary Research 39 229 232
    [Google Scholar]
  7. Gorman B. M., Taylor J. 1978; The RNA genome of Tilligerry virus. Australian Journal of Experimental Biology and Medical Science (in the press)
    [Google Scholar]
  8. Gorman B. M., Walker P. J., Taylor J. 1977; Electrophoretic separation of double-stranded RNA genome segments from Warrego and Mitchell River viruses. Archives of Virology 53 153 158
    [Google Scholar]
  9. Gorman B. M., Waters B., Walker P., Efimoff M. 1973; Biochemistry of the bluetongue-like arboviruses. Report of the Queensland Institute of Medical Research 28 16 17
    [Google Scholar]
  10. Gorman B. M., Leer J. R., Filippich C., Goss P. D., Doherty R. L. 1975; Plaquing and neutralization of arboviruses in the PS-EK. line of cells. Australian Journal of Medical Technology 6 65 71
    [Google Scholar]
  11. Hirst G. K. 1973; Mechanism of influenza recombination. 1. Factors influencing recombination rates between temperature-sensitive mutants of strain WSN and the classification of mutants into complementation-recombination groups. Virology 55 81 93
    [Google Scholar]
  12. Howell P. G. 1966; Some aspects of the epizootiology of bluetongue. Bulletin Office International des Epizooties 66 341 352
    [Google Scholar]
  13. Ikegami N., Gomatos P. J. 1968; Temperature-sensitive conditional lethal mutants of reovirus 3. I. Isolation and characterization. Virology 36 447 458
    [Google Scholar]
  14. Joklik W. K. 1974; Reproduction of reoviridae. In Comprehensive Virology 2 231 334 Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  15. Lecatsas G., Gorman B. M. 1972; Visualization of the extra-capsid coat in certain bluetongue-type viruses. Onderstepoort Journal of Veterinary Research 39 193 196
    [Google Scholar]
  16. Palese P. 1977; The genes of influenza virus. Cell 10 1 10
    [Google Scholar]
  17. Payne C. C., Rivers C. F. 1976; A provisional classification of cytoplasmic polyhedrosis viruses based on the sizes of the RNA genome segments. Journal of General Virology 33 71 85
    [Google Scholar]
  18. Ramig R. F., Cross R. K., Fields B. N. 1977; Genome RNAs and polypeptides of reovirus serotypes 1, 2 and 3. Journal of Virology 22 726 733
    [Google Scholar]
  19. Shatkin A. J., Sipe J. H., Loh P. 1968; Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis. Journal of Virology 2 986 991
    [Google Scholar]
  20. Sharpe A. H., Ramig R. F., Mustoe T. A., Fields B. N. 1978; A genetic map of reovirus. I. Correlation of genome RNAs between serotypes 1, 2 and 3. Virology 84 63 74
    [Google Scholar]
  21. Shipham S. O., de la Rey M. 1976; The isolation and preliminary genetic classification of temperature-sensitive mutants of bluetongue virus. Onderstepoort Journal of Veterinary Research 43 189 192
    [Google Scholar]
  22. Stanley N. F. 1961; Relationships of hepatoencephalomyelitis and reoviruses. Nature, London 189 687
    [Google Scholar]
  23. Suguira A. 1975; Influenza virus genetics. In The Influenza Viruses and Influenza 171 213 Kilbourne E. D. New York: Academic Press;
    [Google Scholar]
  24. Summers W. C. 1970; A simple method for extraction of RNA from E. coli utilizing diethyl pyrocarbonate,. Analytical Biochemistry 33 459 463
    [Google Scholar]
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