1887

Abstract

SUMMARY

The cell-fusing ability of Newcastle disease virus (NDV) was quantified using flow microfluorometry (FMF). The rate of polykaryocyte formation, fusion dependence on multiplicity of infection, and cell fusion differences for 21 NDV strains were measured using this technique. No correlation was found between the virulence of a virus strain and its cell fusion index calculated from the FMF data.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-41-1-27
1978-10-01
2024-12-05
Loading full text...

Full text loading...

/deliver/fulltext/jgv/41/1/JV0410010027.html?itemId=/content/journal/jgv/10.1099/0022-1317-41-1-27&mimeType=html&fmt=ahah

References

  1. Barahona H. H., Hanson R. P. 1968; Plaque enhancement of Newcastle disease virus (lentogenic strains) by magnesium and diethylamino ethyl dextian. Avian Diseases 12:151–158
    [Google Scholar]
  2. Bratt M. A., Gallaher W. R. 1969; Preliminary analysis of the requirements for fusion from within and fusion from without by Newcastle disease virus. Proceedings of the National Academy of Sciences 64:536–543
    [Google Scholar]
  3. Bratt M. A., Rubin H. 1967; Specific interference among strains of Newcastle disease virus. I. Demonstration and measurement of interference. Virology 33:598–608
    [Google Scholar]
  4. Clavell L. A., Bratt M. A. 1972; Hemolytic interaction of Newcastle disease virus and chicken erythro-cytes. Applied Microbiology 23:461–470
    [Google Scholar]
  5. Crissman H. A., Tobey R. A. 1974; Cell-cycle analysis in twenty minutes. Science 184:1297–1298
    [Google Scholar]
  6. Hanson R. P., Brandley C. A. 1955; Identification of vaccine strains of Newcastle disease virus. Science 122:156–157
    [Google Scholar]
  7. Hanson R. P., Spalatin J., Jacobson G. S. 1973; The viscerotropic pathotype of Newcastle disease virus. Avian Diseases 17:354–361
    [Google Scholar]
  8. Holm D. M., Cram L. S. 1973; An improved flow microfluorometer for rapid measurement of cell fluorescence. Experimental Cell Research 80:105–110
    [Google Scholar]
  9. Kohn A., Fuchs P. 1969; Cell fusion by various strains of Newcastle disease and their virulence. Journal of Virology 3:539–540
    [Google Scholar]
  10. Kraemer P. M., Deaven L. L., Crissman H. A., Van Dilla M. A. 1972; DNA constancy despite variability in chromosome number. Advances in Cell and Molecular Biology 2:47–108
    [Google Scholar]
  11. Mullaney P. F., Steinkamp J. A., Crissman H. A., Cram L. S., Holm D. M. 1974; Laser flow micro-fluorometers for rapid analysis and sorting of individual mammalian cells. In Laser Applications in Medicine and Biology151–204 Wolbarsht M. L. New York-London: Plenum Press;
    [Google Scholar]
  12. National Academy Of Sciences-national Research Council 1963 Methods for the Examination of Poultry Biologies Publication 1038 Washington, D.C:
    [Google Scholar]
  13. Reeve P., Poste G. 1971; Studies on the cytopathogenicity of Newcastle disease virus: relation between virulence, polykaryocytosis and plaque size. Journal of General Virology 11:17–24
    [Google Scholar]
  14. Tobey R. A., Crissman H. A., Kraemer P. M. 1972; A method for comparing effects of different synchronizing protocols on mammalian cell cycle traverse. Journal of Cell Biology 54:638–645
    [Google Scholar]
  15. Waterson A. P., Pennington T. H., Allan W. H. 1967; Virulence in Newcastle disease virus. A preliminary study. British Medical Bulletin 23:138–143
    [Google Scholar]
  16. Yeoh G. C. T., Holtzer H. 1977; The effect of cell density, conditioned medium and cytosine arabinoside on myogenesis in primary and secondary cultures. Experimental Cell Research 104:63–78
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-41-1-27
Loading
/content/journal/jgv/10.1099/0022-1317-41-1-27
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error