1887

Abstract

SUMMARY

This report presents evidence which supports a relationship between intracytoplasmic A particles (CAP) and mouse mammary tumour virus (MMTV). Three MMTV-specific antigenic determinants in CAP (MMTV p27, p14 and p10) were detected by immunodiffusion. No structural proteins of comparable mol. wt. were found in CAP; however, exposure of CAP to trypsin resulted in the cleavage of the CAP structural proteins to MMTV-like polypeptides. This process was accompanied by the preservation of MMTV-specific antigenic determinants. Disulphide bonds were necessary for the structural maintenance of CAP. Reducing agents destroyed the organized structure of CAP, whereupon processing of CAP proteins to MMTV-like polypeptides by trypsin was prevented. CAP p82, possessed only MMTV p27 antigenic determinants, while CAP polypeptides p20-18 possessed p10 antigenic determinants. Following processing of CAP structural proteins by trypsin, MMTV-specific p27 antigenic determinants were shifted from CAP p82 to CAP p27; MMTV-p10 antigenic determinants were found with CAP p15-10. These results suggest a model wherein CAP structural proteins are modified by protease during maturation, resulting in the shift of their proteins to sizes consistent with those which have been currently identified as the major internal components of the virion and that this phenomenon is largely predicated on the folding of CAP proteins into the morphologically intact A particle.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-41-1-193
1978-10-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/41/1/JV0410010193.html?itemId=/content/journal/jgv/10.1099/0022-1317-41-1-193&mimeType=html&fmt=ahah

References

  1. Bernhard W., Baoer A., Guerin M., Oberling C. 1955; Etude au microscope electronique de corpuscles d’aspect virusal dans les epitheliomas mammaires de la souris.. Bulletin Cancer 42:163–178
    [Google Scholar]
  2. Cardiff R. D., Puentes M. J., Teramoto Y. A., Lund J. K. 1974; Structure of the mouse mammary tumor virus: characterization of bald particles.. Journal of Virology 14:1293–1303
    [Google Scholar]
  3. Cardiff R. D., Puentes M. J., Young L. J. T., Smith G. H., Teramoto Y. A., Altrock B. W., Pratt T. S. 1978; Serological and biochemical characterization of the mouse mammary tumor virus with localization of p10.. Virology in the press
    [Google Scholar]
  4. Crowle A. J. 1961 In Immunodiffusion222–223 New York: Academic Press;
    [Google Scholar]
  5. Dickson C., Puma J. P., Nandi S. 1976; Identification of a precursor protein to the major glycoproteins of mouse mammary tumor virus.. Journal of Virology 17:275–282
    [Google Scholar]
  6. Gonda M. A., Arthur L. O., Zeve V. H., Fine D. L., Nagashima K. 1976; Surface localization of virus production on a glucocorticoid-stimulated oncornavirus-producing mouse mammary tumor cell line by scanning electron microscopy.. Cancer Research 36:1084–1093
    [Google Scholar]
  7. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent.. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  8. Maizel J. V. jun. 1971; Polyacrylamide gel electrophoresis of viral proteins.. In Methods of Virology v179–246 Maramorosch K., Koprowski H. New York: Academic Press;
    [Google Scholar]
  9. Nowinski R., Sarkar N., Old L., Moore D. H., Scheer D., Hilgers J. 1971; Characteristics of the structural components of the mouse mammary tumor virus. II. Viral proteins and antigens.. Virology 46:21–48
    [Google Scholar]
  10. Parks W. P., Howk R. S., Scolnick E. M., Oroszlan S., Gilden R. 1974; Immunochemical characterization of two major polypeptides from murine mammary tumor virus.. Journal of Virology 13:1200–1210
    [Google Scholar]
  11. Parks W. P., Ranson J. C., Young H. A., Scolnick E. M. 1975; Mammary tumor virus induction by glucocorticoid: characterization of specific transcriptional regulation.. Journal of Biological Chemistry 250:3330–3336
    [Google Scholar]
  12. Poureau-Schneider N. 1967; Cytoplasmic inclusions in estrogen-induced testicular interstitial-cell tumors in mice.. Journal of the National Cancer Institute 39:67–74
    [Google Scholar]
  13. Smith G. H., Wivel N. A. 1972; Isolation and partial characterization of intracytoplasmic A particles.. Virology 48:270–274
    [Google Scholar]
  14. Smith G. H., Wivel N. A. 1973; Intracytoplasmic A particles. Mouse mammary tumor virus nucleoprotein cores?. Journal of Virology 11:575–584
    [Google Scholar]
  15. Smith G. H., Litwack M., Longfellow D. G. 1974; High molecular weight DNA associated with intracytoplasmic A particle inclusions.. Cancer Research 34:2654–2662
    [Google Scholar]
  16. Smith G. H., Lee B. K. 1975; Mouse mammary tumor virus polypeptide precursors in intracytoplasmic A particles.. Journal of the National Cancer Institute 55:493–496
    [Google Scholar]
  17. Tanaka H. 1977; Precursor-product relationship between nonglycosylated polypeptides of A and B particles of mouse mammary tumor virus.. Virology 76:835–850
    [Google Scholar]
  18. Teramoto Y. A., Puentes M. J., Young L. J. T., Cardiff R. D. 1974; Structure of the mouse mammary tumor virus: Polypeptides and glycoproteins.. Journal of Virology 13:411–418
    [Google Scholar]
  19. Teramoto Y. A., Cardiff R. D., Lund J. K. 1977; The structure of the mouse mammary tumor virus: isolation and characterization of the core.. Virology 77:135–148
    [Google Scholar]
  20. Vogt V. M., Eisenman R., Diggelman H. 1975; Generation of avian myeloblastosis virus structural proteins by proteolytic cleavage of a precursor polypeptide.. Journal of Molecular Biology 96:471–493
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-41-1-193
Loading
/content/journal/jgv/10.1099/0022-1317-41-1-193
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error