1887

Abstract

Summary

The Amicon TCIR recycle system with Diaflo PM30 filters provided a rapid, simple and inexpensive method for the concentration of baboon endogenous virus from a large quantity of culture medium from chronically infected cells. The recovery of infectivity and virion RNA-dependent DNA polymerase activity was substantially better than after the conventional concentration methods of ultra-centrifugation or precipitation with ammonium sulphate or polyethylene glycol 6000. The combination of ultrafiltration and Ficoll gradient centrifugation is recommended as a general procedure for the purification of fragile C-type viruses.

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/content/journal/jgv/10.1099/0022-1317-41-1-135
1978-10-01
2019-11-16
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-41-1-135
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