1887

Abstract

SUMMARY

The Amicon TCIR recycle system with Diaflo PM30 filters provided a rapid, simple and inexpensive method for the concentration of baboon endogenous virus from a large quantity of culture medium from chronically infected cells. The recovery of infectivity and virion RNA-dependent DNA polymerase activity was substantially better than after the conventional concentration methods of ultra-centrifugation or precipitation with ammonium sulphate or polyethylene glycol 6000. The combination of ultrafiltration and Ficoll gradient centrifugation is recommended as a general procedure for the purification of fragile C-type viruses.

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1978-10-01
2024-04-25
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References

  1. Baltimore D. 1970; Viral RNA-dependent DNA polymerase. Nature, London 226:1209–1211
    [Google Scholar]
  2. Barringer H. P. 1966; The design of zonal centrifuges. National Cancer Institute Monograph 21:77–111
    [Google Scholar]
  3. Benveniste R. E., Lieber M. M., Livingston D. M., Sherr C. J., Todaro G. J., Kalter S. S. 1974; Infectious C-type virus isolated from a baboon placenta. Nature, London 248:17–20
    [Google Scholar]
  4. Bronson D. L., Elliott A. Y., Ritzi D. 1976; A comparison of four methods used to concentrate Rous sarcoma virus from tissue culture fluids. Journal of General Virology 33:403–410
    [Google Scholar]
  5. Duesberg P. H., Robinson H. L., Robinson W. L. 1968; Proteins of Rous sarcoma virus. Virology 36:73–86
    [Google Scholar]
  6. Giard D. J., Aaronson S. A., Todaro G. J., Arnstein P., Kersey J. H., Dosik H., Parks W. P. 1973; In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. Journal of the National Cancer Institute 51:1417–1423
    [Google Scholar]
  7. Green M., Rokutanda M., Fujinaga K., Ray R. K., Rokutanda H., Gurgo C. 1970; Mechanism of carcinogenesis by RNA tumor viruses, 1. An RNA-dependent DNA polymerase in murine sarcoma viruses. Proceedings of the National Academy of Sciences of the United States of America 67:385–393
    [Google Scholar]
  8. House W. 1973; Bulk culture of cell monolayers. In Tissue Culture338–444 Kruse P. F. Jun, Patterson M. K. Jun New York: Academic Press;
    [Google Scholar]
  9. Klingeborn B., Pertoft H. 1972; Equine abortion (Herpes) virus: purification and concentration of enveloped and deenveloped virus and envelope material by density gradient centrifugation in colloidal silica. Virology 48:618–623
    [Google Scholar]
  10. Layne E. 1957; Protein estimation by ultraviolet absorption. In Methods in Enzymology III452–454 Colowick S. P., Kaplan N. O. New York: Academic Press;
    [Google Scholar]
  11. Nowinski R. C., Old L. J., Moore D. H., Geering G., Boyse E. A. 1967; A soluble antigen of the mammary tumor virus. Virology 31:1–14
    [Google Scholar]
  12. Rhim J. S., Williams L. B. Jun, Huebner R. J., Turner H. C. 1969; Concentration by Diaflo ultrafiltration of murine leukemia and sarcoma viruses grown in tissue cultures. Cancer Research 29:154–156
    [Google Scholar]
  13. Spiegelman S., Schlom J., Burny A., Travnicek M., Das M. R., Watson K., Keydar J. 1970; Synthetic DNA-RNA hybrids and RNA-RNA duplexes as templates for the polymerases of the oncogenic RNA viruses. Nature, London 228:430–432
    [Google Scholar]
  14. Temin H. M., Mizutani S. 1970; RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature, London 226:1211–1213
    [Google Scholar]
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