1887

Abstract

Summary

The association of newly synthesized vaccinia virus DNA with proteins in infected HeLa cells was followed. A shift from a high density to a low density complex occurred between 3 and 6 h after infection. This process was not affected by isatin β thiosemicarbazone (IBT), an inhibitor of pox virus growth. At 22 h after infection in the absence of IBT, mature virions of high density were observed; however, in the presence of the drug, high density DNA-protein complexes, which lack the two main virus core polypeptides, were formed.

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1978-09-01
2022-01-16
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References

  1. Appleyard G., Hume V. B. M., Westwood J. C. N. 1965; The effect of thiosemicarbazones on the growth of rabbit pox virus in tissue culture. Annals of the New York Academy of Sciences 130:92–104
    [Google Scholar]
  2. Dahl R., Kates J. R. 1970; Intracellular structures containing vaccinia DNA: isolation and characterization. Virology 42:453–462
    [Google Scholar]
  3. Easterbrook K. B. 1961; Interference with the maturation of vaccinia virus by isatin β thiosemicarbazone. Virology 17:245–251
    [Google Scholar]
  4. Holowczak J. A., Joklik W. K. 1967; Studies on the structural proteins of vaccinia virus. I. Structural proteins of virions and cores. Virology 33:717–725
    [Google Scholar]
  5. Joklik W. K., Becker Y. 1964; The replication and coating of vaccinia DNA. Journal of Molecular Biology 10:452–474
    [Google Scholar]
  6. Katz E., Moss B. 1970; Vaccinia virus structural polypeptide derived from a high molecular weight precursor: formation and integration into virus particles. Journal of Virology 6:717–726
    [Google Scholar]
  7. Katz E., Margalith E., Winer B., Goldblum N. 1973a; Synthesis of vaccinia virus polypeptides in the presence of isatin β thiosemicarbazone. Antimicrobial Agents and Chemotherapy 4:44–48
    [Google Scholar]
  8. Katz E., Margalith E., Winer B., Lazar A. 1973b; Characterization and mixed infection of three strains of vaccinia virus: wild type, IBT-resistant and IBT-dependent mutants. Journal of General Virology 21:469–475
    [Google Scholar]
  9. Sheffield F. W., Bauer D. J., Stephenson S. 1960; The protection of tissue culture by isatin β thiosemicarbazone from the cytopathic effects of certain pox viruses. The British Journal of Experimental Pathology 41:638–647
    [Google Scholar]
  10. Summers D. F., Maizel J. V. Jun, Darnell J. E. Jun 1965; Evidence for virus specific non-capsid proteins in poliovirus infected HeLa cells. Proceedings of the National Academy of Sciences of the United States of America 54:505–513
    [Google Scholar]
  11. Woodson B., Joklik W. K. 1965; The inhibition of vaccinia virus multiplication by isatin β thiosemicarbazone. Proceedings of the National Academy of Sciences of the United States of America 54:946–953
    [Google Scholar]
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