Morphological revertants have been isolated from one line of adenovirus type 12-transformed hamster cells. This line, T637, is oncogenic in hamsters and contains multiple copies of the virus genome per cell. Different parts of the virus genome are represented in non-stoichiometric amounts and the virus DNA persists in the cells in an integrated form. The pattern of integrated virus genomes has been determined by the blotting technique.
In the T637 line, morphological revertants arise spontaneously at relatively high frequency. Two of these revertants have been cloned. In contrast to the T637 line, the revertants F10 and G12 exhibit fibroblastic morphology. The patterns of integrated virus genomes in the revertants differ markedly from that of the T637 line; one of the revertant cell lines, F10, appears to have lost all virus DNA sequences. The morphological revertants continue to express the oncogenic phenotype, although the time required to produce tumours in animals appears to be prolonged compared to the parental BHK21 and the T637 cell lines. A number of biological parameters of the revertant lines have also been investigated.
BablanianR.,
EggersH. J.,
TammI.1965; Studies on the mechanism of poliovirus-induced cell damage: I. The relation between poliovirus-induced metabolic and morphological alterations in cultured cells. Virology 26:100–113
BurgerH.,
DoerflerW.1974; Intracellular forms of adenovirus DNA. III. Integration of the DNA of adenovirus type 2 into host DNA in productively infected cells. Journal of Virology 13:975–992
ChenL. B.,
GallimoreP. H.,
McDougallJ. K.1976; Correlation between tumor induction and the large external transformation sensitive protein on the cell surface. Proceedings of the National Academy of Sciences of the United States of America 73:3570–3574
DoerflerW.,
BaczkoK.,
FanningE.,
GronebergJ.,
OrtinJ.,
ScheidtmannK. H.,
SchickJ.,
SobollH.1977; Integration of adenovirus DNA into the host genome. Bulletin de l’ Institut Pasteur 75:141–185
FanningE.,
DoerflerW.1976; Intracellular forms of adenovirus DNA. V. Viral DNA sequences in hamster cells abortively infected and transformed with human adenovirus type 12. Journal of Virology 20:373–383
FosterD. O.,
PardeeA. B.1969; Transport of amino acids by confluent and non-confluent 3T3 and polyoma virus-transformed 3T3 cells growing on glass cover slips. Journal of Biological Chemistry 244:2675–2681
GreeneP. J.,
BetlachM. C.,
GoodmanH. M.,
BoyerH. W.1974; The Eco RI restriction endonuclease. Methods in Molecular Biology vol 6 pp 413–428 Edited by
WicknerR.
New York: Academic Press Inc;
KetnerG.,
KellyT. J.Jun1976; Integrated simian virus 40 sequences in transformed cell DNA: analysis using restriction endonucleases. Proceedings of the National Academy of Sciences of the United States of America 73:1102–1106
ManiatisT.,
JeffreyA.,
KleidD. G.1975; Nucleotide sequence of the rightward operator of phage λ. Proceedings of the National Academy of Sciences of the United States of America 72:1184–1188
OrtinJ.,
ScheidtmannK. H.,
GreenbergR.,
WestphalM.,
DoerflerW.1976; Transcription of the genome of adenovirus type 12. III. Maps of stable RNA from productively infected human cells and abortively infected and transformed hamster cells. Journal of Virology 20:355–372
RigbyP. W. J.,
DieckmannM.,
RhodesC.,
BergP.1977; Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
SteinbergB.,
PollackR.,
ToppW.,
BotchanM.1978; Isolation and characterization of T antigennegative revertants from a line of transformed rat cells containing one copy of the SV40 genome. Cell 13:19–32
StrohlW. A.,
RouseH.,
TeetsK.,
SchlesingerR. W.1970; The response of BHK21 cells to infection with type 12 adenovirus. III. Transformation and restricted replication of superinfecting type 2 adenovirus. Archiv für die gesamte Virusforschung 31:93–112
SutterD.,
WestphalM.,
DoerflerW.1978; Patterns of integration of viral DNA sequences in the genomes of adenovirus type 12-transformed hamster cells. Cell 14:569–585
WilsonG. A.,
YoungF. E.1975; Isolation of a sequence-specific endonuclease (Bam I) from Bacillus amyloliquefaciens H.. Journal of Molecular Biology 97:123–125