1887

Abstract

SUMMARY

Translation of the satellite RNA (RNA-3) of tomato black ring virus (TBRV) in wheat germ extracts or reticulocyte lysates resulted in the synthesis of a polypeptide of mol. wt. about 48000, both in the presence and in the absence of RNA-1 and RNA-2. The RNA-3 specific polypeptide of TBRV strain G was slightly larger than that of strain S. A polypeptide of the same electrophoretic mobility as the translation product of RNA-3 was found in extracts of protoplasts infected with an isolate of TBRV-S possessing RNA-3 but not in extracts of protoplasts infected with an isolate lacking RNA-3. The lack of phenotypic effect of RNA-3 raises the question of the function of this protein in the infected plant.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-40-3-587
1978-09-01
2021-10-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/40/3/JV0400030587.html?itemId=/content/journal/jgv/10.1099/0022-1317-40-3-587&mimeType=html&fmt=ahah

References

  1. Delbos R., Dunez J., Barrau J., Fisac R. 1976; The RNAs of three strains of tomato black ring virus. Annales de Microbiologie. (Institut Pasteur) 127A:101–109
    [Google Scholar]
  2. Harrison B. D. 1958; Relationship between beet ringspot, potato bouquet and tomato black ring viruses. Journal of General Microbiology 18:450–460
    [Google Scholar]
  3. Harrison B. D., Murant A. F. 1977; Nepovirus group. Commonwealth Mycological Institute/Association of Applied Biologists Descriptions of Plant Viruses No. 185 4 pp
    [Google Scholar]
  4. Hatchard C. O., Parker C. A. 1956; A new sensitive chemical actinometer II. Potassium ferrioxalate as a standard chemical actinometer. Proceedings of the Royal Society, London, Series A 235:518–536
    [Google Scholar]
  5. Hunter A. R., Farrell P. J., Jackson R. J., Hunt T. 1977; The role of polyamines in cell-free protein synthesis in the wheat germ system. European Journal of Biochemistry 75:149–157
    [Google Scholar]
  6. Hunter A. R., Hunt T., Knowland J., Zimmern D. 1976; Messenger RNA for the coat protein of tobacco mosaic virus. Nature, London 260:759–764
    [Google Scholar]
  7. Kaper J. M., Tousignant M. E., Lot H. 1976; A low molecular weight replicating RNA associated with a divided genome plant virus: defective or satellite RNA?. Biochemical and Biophysical Research Communications 72:1237–1243
    [Google Scholar]
  8. Kassanis B. 1966; Properties and behaviour of satellite virus. In Viruses of Plants Proceedings of the International Conference on Plant Viruses Wageningen 1965, pp 177–187 Edited by Beemster A. B. R., Dijkstra J. Amsterdam: North-Holland;
    [Google Scholar]
  9. Klein C., Fritsch C., Briand J. P., Richards K. E., Jonard G., Hirth L. 1976; Physical and functional heterogeneity in TYMV RNA: evidence for the existence of an independent messenger coding for coat protein. Nucleic Acids Research 3:3043–3061
    [Google Scholar]
  10. Kubo S., Harrison B. D., Barker H. 1975a; Defined conditions for growth of tobacco plants as sources of protoplasts for virus infection. Journal of General Virology 28:255–257
    [Google Scholar]
  11. Kubo S., Harrison B. D., Robinson D. J., Mayo M. A. 1975b; Tobacco rattle virus in tobacco mesophyll protoplasts: infection and virus multiplication. Journal of General Virology 27:293–304
    [Google Scholar]
  12. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  13. Marcu K., Dudock B. 1974; Characterization of a highly efficient protein synthesizing system derived from commercial wheat germ. Nucleic Acids Research 1:1385–1397
    [Google Scholar]
  14. Mayo M. A., Fritsch C., Hirth L. 1976; Translation of tobacco rattle virus RNA in vitro using wheat germ extracts. Virology 69:408–415
    [Google Scholar]
  15. Mohier E., Hirth L., Lemeur M. A., Gerlinger P. 1975; Translation of alfalfa mosaic virus RNAs in mammalian cell-free systems. Virology 68:349–359
    [Google Scholar]
  16. Murant A. F., Mayo M. A., Harrison B. D., Goold R. A. 1973; Evidence for two functional RNA species and a ‘satellite’ RNA in tomato black ring virus. Journal of General Virology 19:275–278
    [Google Scholar]
  17. Murant A. F., Taylor M. 1978; Estimates of molecular weights of repovirus RNA species by polyacrylamide gel electrophoresis under denaturing conditions. Journal of General Virology41 (in the press)
    [Google Scholar]
  18. Owens R. A., Kaper J. M. 1977; Cucumber mosaic virus-associated RNAs. II. In vitro translation in a wheat germ protein-synthesis system. Virology 80:196–203
    [Google Scholar]
  19. Owens R. A., Schneider I. R. 1977; Satellite of tobacco ringspot virus RNA lacks detectable mRNA activity. Virology 79:222–224
    [Google Scholar]
  20. Pleij C. W. A., Neeleman A., van Vloten-Doting L., Bosch L. 1976; Translation of turnip yellow mosaic virus RNA in vitro: a closed and an open coat protein cistron. Proceedings of the National Academy of Sciences of the United States of America 73:4437–4441
    [Google Scholar]
  21. Randles J. W., Harrison B. D., Murant A. F., Mayo M. A. 1977; Packaging and biological activity of the two essential RNA species of tomato black ring virus. Journal of General Virology 36:187–193
    [Google Scholar]
  22. Sakai F., Watts J. W., Dawson J. R. O., Bancroft J. B. 1977; Synthesis of proteins in tobacco protoplasts infected with cowpea chlorotic mottle virus. Journal of General Virology 34:285–293
    [Google Scholar]
  23. Salvato M. S., Fraenkel-Conrat H. 1977; Translation of tobacco necrosis virus and its satellite in a cell-free wheat germ system. Proceedings of the National Academy of Sciences of the United States of America 74:2288–2292
    [Google Scholar]
  24. Schneider I. R. 1969; Satellite-like particle of tobacco ringspot virus that resembles tobacco ringspot virus. Science 166:1627–1629
    [Google Scholar]
  25. Schneider I. R. 1971; Characteristics of a satellite-like virus of tobacco ringspot virus. Virology 45:108–122
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-40-3-587
Loading
/content/journal/jgv/10.1099/0022-1317-40-3-587
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error