1887

Abstract

SUMMARY

The stability of the infectivity of the non-occluded virions (NOV) of the nuclear polyhedrosis virus (Ac-NPV) was tested. Exposure of Ac-NPV NOV to solutions ranging from pH 7.5 to 11.4 with molarities of 0.1 to 0.001 showed that infectivity was most stable in 0.001 , pH 7.5, tris(hydroxymethyl)aminomethane (tris)-HCl buffer. Infectious NOV could be concentrated by precipitation with 50% saturated ammonium sulphate or 9% (w/w) polyethylene glycol 4000. Treatment of concentrated preparations with 1 -urea did not affect infectivity or the centrifugal properties of the NOV. NOV were partially purified by centrifugation through a sucrose underlay.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-40-2-493
1978-08-01
2021-09-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/40/2/JV0400020493.html?itemId=/content/journal/jgv/10.1099/0022-1317-40-2-493&mimeType=html&fmt=ahah

References

  1. Brown M., Faulkner P. 1977; A plaque assay for nuclear polyhedrosis viruses using a solid overlay. Journal of General Virology 36:361–364
    [Google Scholar]
  2. Dougherty E. M., Vaughn J. L., Reichelderfer C. F. 1975; Characteristics of the non-occluded form of a nuclear polyhedrosis virus. Intervirology 5:109–121
    [Google Scholar]
  3. Harrap K. A., Longworth J. F. 1974; An evaluation of purification methods for baculoviruses. Journal of Invertebrate Pathology 24:55–62
    [Google Scholar]
  4. Henderson J. F., Faulkner P., Mackinnon E. A. 1974; Some biophysical properties of virus present in tissue cultures infected with the nuclear polyhedrosis virus of Trichoplusia ni . Journal of General Virology 22:143–146
    [Google Scholar]
  5. Hink W. F. 1970; Established insect cell line from the cabbage looper, Trichoplusia ni . Nature, London 226:446–467
    [Google Scholar]
  6. Hink W. F., Vail P. V. 1973; A plaque assay for titration of alfalfa looper nuclear polyhedrosis virus in a cabbage looper (TN-368) cell line. Journal of Invertebrate Pathology 22:168–174
    [Google Scholar]
  7. Kawanishi C. Y., Paschke J. D. 1970; The relationship of buffer pH and ionic strength on the yield of virions and nucleocapsids obtained by the dissolution of Rachiplusia ou nuclear polyhedra. Proceedings of the 4th International Colloquium on Insect Pathology College Park; Md. p 127–146
    [Google Scholar]
  8. Volkman L. E., Summers M. D., Hsieh C.-H. 1976; Occluded and nonoccluded nuclear polyhedrosis virus grown in Trichoplusia ni: comparative neutralization, comparative infectivity, and in vitro growth studies. Journal of Virology 19:820–832
    [Google Scholar]
  9. Wood H. A. 1977; An agar overlay plaque assay method for Autographa californica nuclear-polyhedrosis virus. Journal of Invertebrate Pathology 29:304–307
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-40-2-493
Loading
/content/journal/jgv/10.1099/0022-1317-40-2-493
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error