1887

Abstract

Summary

The stability of the infectivity of the non-occluded virions (NOV) of the nuclear polyhedrosis virus (Ac-NPV) was tested. Exposure of Ac-NPV NOV to solutions ranging from pH 7.5 to 11.4 with molarities of 0.1 to 0.001 showed that infectivity was most stable in 0.001 , pH 7.5, tris(hydroxymethyl)aminomethane (tris)-HCl buffer. Infectious NOV could be concentrated by precipitation with 50% saturated ammonium sulphate or 9% (w/w) polyethylene glycol 4000. Treatment of concentrated preparations with 1 -urea did not affect infectivity or the centrifugal properties of the NOV. NOV were partially purified by centrifugation through a sucrose underlay.

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/content/journal/jgv/10.1099/0022-1317-40-2-493
1978-08-01
2019-11-20
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-40-2-493
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