The enzyme linked immunosorbent assay (ELISA) has been shown to be a specific and sensitive method to detect a nuclear polyhedrosis virus in Heliothis armigera larvae. 1 ng/ml of purified virus particle antigen could be detected. The presence of larval extracts did not interfere in the assay. The growth of the virus in Heliothis armigera larvae was analysed by ELISA and the conventional counting of virus polyhedra and this showed that ELISA was a superior detection method.
BrownD. A.,
BudH. M.,
KellyD. C.1977; Biophysical properties of the structural components of a granulosis virus isolated from the cabbage white butterfly (Pieris brassicae). Virology 81:317–327
ClarkM. F.,
AdamsA. N.1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
CrawfordA. M.,
KalmakoffJ.1977; Effect of alkaline protease on the antigenic nature of Wiseana nuclear polyhedrosis virus polyhedron protein. Journal of Virology 24:412–415
KalmakoffJ.,
CrawfordA. M.,
MooreS. G.1977; A radioimmune assay using labelled antibody for polyhedron protein from a nuclear polyhedrosis virus of Wiseana cervinata
. Journal of Invertebrate Pathology 29:31–35
KellyD. C.,
EdwardsM.-L.,
RobertsonJ. S.1978; The use of enzyme linked immunosorbent assay to detect and discriminate between small iridescent viruses. Annals of Applied Biology (in the press)
SummersM. D.,
EnglerR.,
FalconL. A.,
VailP.1975 In Baculoviruses for Insect Pest Control: Safety Considerations published by the American Society for Microbiology;186 pp
VollerA.,
BartletA.,
Bid WellD. E.,
ClarkM. F.,
AdamsA. N.1976a; The detection of viruses by enzyme linked immunosorbent assay. Journal of General Virology 33:165–167
VollerA.,
BidwellD. E.,
BartlettA.1976b; Enzyme immunoassays in diagnostic medicine: theory and practice. Bulletin of the World Health Organisation 53:55–65
WigleyP. J.1976; The epizootiology of a nuclear polyhedrosis virus disease of the winter moth. Operophtera brumata L., at Wistman’s Wood, Dartmoor D. Phil, thesis University of Oxford;