Surface antigens of BHK-21 cells infected with HSV-1 or HSV-2 were radio-iodinated (I) with lactoperoxidase, immune precipitated and analysed by polyacrylamide gel electrophoresis (PAGE). Experiments using antiserum to HSV-1 or HSV-2, absorbed with appropriate homotypic or heterotypic antigens, revealed that both type-specific (homotypic) and cross-reactive antibody combined with surface glycoproteins to form a single large radioactive peak. This peak, which constituted the major glycoprotein region (region ) observed in electropherograms, represented a range in mol. wt. from 115000 to 130000. Sensitization of cells to complement lysis, neutralization of infectious virus and immune precipitation of surface glycoproteins (region ) were found to be generally correlated properties of all the antibody preparations analysed, including antibody prepared specifically against region antigens. These findings suggest a major immunological role for the surface glycoproteins migrating in PAGE region .


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