1887

Abstract

SUMMARY

The replication of human cytomegalovirus (CMV) and herpes simplex virus (HSV) was studied in three human embryo cell lines (CMV-Mj-HEL-1, CMV-Mj-HEL-2, and CMV-Mj-HEL-2,T-1) transformed by human CMV. Growth studies revealed that these cells were completely resistant to infection by CMV strains AD169 and Mj and partially resistant to HSV types 1 and 2. Neither virus DNA nor virus proteins were synthesized in the transformed cells infected with CMV AD169. The HSV production in CMV-transformed human embryo lung (HEL) cells was delayed when compared to the virus production in normal HEL cells and spread of HSV c.p.e. was slower in the transformed cells. The treatment of normal HEL cells with a crude extract of CMV-transformed HEL cells also resulted in inhibition of the spread of c.p.e. of HSV types 1 and 2. The inhibitory effect was not due to interferon since vesicular stomatitis virus replication was not affected and several experiments showed that it was not due to mycoplasma. The presence of virus inhibitor molecules in CMV-transformed cells absent in normal HEL cells is postulated.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-40-2-391
1978-08-01
2022-01-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/40/2/JV0400020391.html?itemId=/content/journal/jgv/10.1099/0022-1317-40-2-391&mimeType=html&fmt=ahah

References

  1. Andersson M., Lindahl T. 1976; Epstein-Barr virus DNA in human lymphoid cell lines: in vitro conversion. Virology 73:96–105
    [Google Scholar]
  2. Darai G., Munk K. 1976; Neoplastic transformation of rat embryo cells with herpes simplex virus. International Journal of Cancer 18:469–481
    [Google Scholar]
  3. Doller E. W. 1977; Evidence for an inhibitor of herpes simplex virus in transformed hamster cells. Proceedings of the Society for Experimental Biology and Medicine 154:168–170
    [Google Scholar]
  4. Doller E., Duff R., Rapp F. 1973; Resistance of hamster cells transformed by herpes simplex virus 2 to superinfection by herpes simplex viruses. Intervirology 1:154–167
    [Google Scholar]
  5. Furukawa T., Tanaka S., Plotkin S. A. 1975; Stimulation of macromolecular synthesis in guinea pig cells by human CMV. Proceedings of the Society for Experimental Biology and Medicine 148:211–214
    [Google Scholar]
  6. Geder L., Lausch R., O’Neill F., Rapp F. 1976; Oncogenic transformation of human embryo lung cells by human cytomegalovirus. Science 192:1134–1137
    [Google Scholar]
  7. Geder L., Laychock A., Gorodecki J., Rapp F. 1978; Alteration in biological properties of different lines of cytomegalovirus-transformed human embryo cells following in vitro cultivation. In Oncogenesis and Herpesviruses (in the press) Edited by de The G., Henle W., Rapp F. Lyon: IARC;
    [Google Scholar]
  8. Gluzman Y., Davison J., Oren M., Winocours E. 1977; Properdes of permissive monkey cells transformed by UV-irradiated simian virus 40. Journal of Virology 22:256–266
    [Google Scholar]
  9. Lowry O. H., Rosebrough N. J., Farr A. G., Randall R. J. 1951; Protein measurement with the Folinreagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  10. Rapp F., Geder L., Murasko D., Lausch R., Ladda R., Huang E. S., Webber M. M. 1975; Long-term persistence of cytomegalovirus genome in cultured human cells of prostatic origin. Journal of Virology 1 6:982–990
    [Google Scholar]
  11. Rapp F., Trulock S. C. 1970; Susceptibility to superinfection of simian cells transformed by SV40. Virology 40:961–970
    [Google Scholar]
  12. St. Jeor S. C., Albrecht T. B., Funk F. D., Rapp F. 1974; Stimulation of cellular DNA synthesis by human cytomegalovirus. Journal of Virology 13:353–362
    [Google Scholar]
  13. St. Jeor S., Rapp F. 1973; Cytomegalovirus replication in cells pretreated with 5-iodo-2′-deoxyuridine. Journal of Virology II:986–990
    [Google Scholar]
  14. Sambrook J. 1972; Transformation by polyoma virus and simian virus 40. Advances in Cancer Research 1 6:141–180
    [Google Scholar]
  15. Vogt P. K., Ishizaki R. 1966; Patterns of viral interference in the avian leukosis and sarcoma complex. Virology 30:368–374
    [Google Scholar]
  16. Wentworth B. B., French L. 1970; Plaque assay of cytomegalovirus strains of human origin. Proceedings of the Society for Experimental Biology and Medicine 135:253–258
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-40-2-391
Loading
/content/journal/jgv/10.1099/0022-1317-40-2-391
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error