1887

Abstract

SUMMARY

Serological relationships between plant viruses within six different taxonomic groups were studied by means of the double antibody sandwich form of enzyme-linked immunosorbent assay (ELISA). This technique was very sensitive not only for the detection of low concentrations of viruses, but also for the differentiation of closely related antigens. With some viruses, e.g. Andean potato latent virus, the specificity of the test was so great that conjugates prepared to one strain failed to detect other serologically closely related strains. With other viruses and with host proteins the cross-reactivity was broader, but distant and even intermediate serological relationships between viruses were usually not detected. The great specificity of ELISA is mainly caused by the behaviour of the conjugated antibodies. The binding of conjugated antibodies was completely inhibited by simultaneously added native antibodies. Antibodies from very early bleedings were not suitable for ELISA.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-40-2-309
1978-08-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/40/2/JV0400020309.html?itemId=/content/journal/jgv/10.1099/0022-1317-40-2-309&mimeType=html&fmt=ahah

References

  1. Casper R. 1977; Testung von Prunus avium-Samen auf prune dwarf virus mit dem ELISA-Verfahren. Phytopathologische Zeitschrift 90:91–94
    [Google Scholar]
  2. Clark M. F., Adams A. N., Thresh J. M., Casper R. 1976; The detection of plum pox and other viruses in woody plants by enzyme-linked immunosorbent assay (ELISA). Acta Horticulturae 67:51–57
    [Google Scholar]
  3. Clark M. F., Adams A. N. 1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
    [Google Scholar]
  4. Fribourg C. E., Jones R. A. C., Koenig R. 1977; Host plant reactions, physical properties and serology of three isolates of Andean potato latent virus from Peru. Annals of Applied Biology 86:373–380
    [Google Scholar]
  5. Gibbs A. J., Harrison B. D. 1973; Eggplant mosaic virus. CMI/AAB Descriptions of Plant Viruses No. 124 4 pp
    [Google Scholar]
  6. Koenig R. 1972; Anomalous behavior of the coat proteins of potato virus X and cactus virus X during electrophoresis in dodecylsulfate containing polyacrylamide gels. Virology 50:263–266
    [Google Scholar]
  7. Koenig R. 1976; A loop-structure in the serological classification system of tymoviruses. Virology 72:1–5
    [Google Scholar]
  8. Koenig R., Bode O. 1978; Sensitive detection of Andean potato latent and Andean potato mottle viruses in potato tubers with the serological latex-test. Phytopathologische Zeitschrift (in the press)
    [Google Scholar]
  9. Koenig R., Stegemann H., Francksen H., Paul H. L. 1970; Protein subunits in the potato virus X group. Determination of the molecular weights by polyacrylamide electrophoresis. Biochimica et Biophysica Acta 207:184–189
    [Google Scholar]
  10. Koenig R., Tremaine J. H., Shepard J. F. 1978; In situ degradation of the protein chain of potato virus X at the N- and the C-termini. Journal of General Virology 38:329–337
    [Google Scholar]
  11. Kuschki S. H., Koeniq R., Duvel D., Kuhne H. 1978; Helenium virus S and Y –two new viruses from commercially grown Helenium hybrids. Phytopathology (in the press)
    [Google Scholar]
  12. Miličić D., Pleše N., Bercks R., Brandes J., Casper R., Chessin M. 1966; Vergleichende serologische und elektronenmikroskopische Untersuchungen an Isolaten des Kakteen-X-Virus. Phytopathologische Zeitschrift 55:211–217
    [Google Scholar]
  13. Thresh J. M., Adams A. N., Barbara D. J., Clark M. F. 1977; The detection of three viruses of hop (Humulus lupulus) by enzyme-linked immunosorbent assay. Annals of Applied Biology 87:57–65
    [Google Scholar]
  14. Voller A., Bartlett A., Bid Well D. E., Clark M. F., Adams A. N. 1976; The detection of Viruses by enzyme-linked immunosorbent assay (ELISA). Journal of General Virology 33:165–167
    [Google Scholar]
  15. Woodhead L. S., Addison G. M., Hales C. N. 1974; The immunoradiometric assay and related techniques. British Medical Bulletin 30:44–49
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-40-2-309
Loading
/content/journal/jgv/10.1099/0022-1317-40-2-309
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error