1887

Abstract

SUMMARY

Plaque neutralization assays were carried out using the rhabdoviruses of pike fry (2 isolates), spring viraemia of carp and grass carp. When rabbit anti-pike fry rhabdovirus sera were used, no neutralizing activity was observed but plaque counts increased with heat-inactivated sera or sera that had been stored at -20 °C for prolonged periods. Antibody binding to the virus was demonstrated by gradient centrifugation experiments. The use of immune serum alone or in combination with anti-rabbit gamma globulin serum resulted in aggregation of radioactive virus. Aggregation was also observed when anti-rabbit gamma globulin serum was added under neutralization test conditions. Addition of non-inactivated guinea-pig serum to mixtures of pike fry rhabdovirus and rabbit antiserum to spring viraemia of carp virus resulted in strong neutralization, whereas this antiserum alone or in the presence of inactivated guinea-pig serum gave an increase in plaque numbers. The increase in plaque number and the complement-dependent neutralization phenomena were weak or absent in the homologous spring viraemia of carp virus system.

Immunization of pike with pike fry rhabdovirus resulted in an increase in virus neutralizing activity which could be attributed to the macroglobulin fraction of the serum. After immunizations over a period of 6 months with virus antigens, no further increase in neutralizing activity was observed. Pike sera showed 50% plaque reduction at concentrations between 0.017 and 0.3%. Using these sera it could be demonstrated that the grass carp virus and the V 75/94 isolate from pike fry were indistinguishable from the original pike fry rhabdovirus, whereas spring viraemia of carp virus was different.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-40-2-297
1978-08-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/40/2/JV0400020297.html?itemId=/content/journal/jgv/10.1099/0022-1317-40-2-297&mimeType=html&fmt=ahah

References

  1. Ahne W. 1975; A rhabdovirus isolated from grass carp (Ctenopharyngodon idella Val.). Archives of Virology 48:181–185
    [Google Scholar]
  2. Almeida J. D., Waterson A. P. 1969; The morphology of virus-antibody interaction. Advances in Virus Research 15:307–338
    [Google Scholar]
  3. Ashe W. K., Notkins A. L. 1967; Kinetics of senzitization of herpes simplex virus and its relationship to the reduction in the neutralization rate constant. Virology 33:616–617
    [Google Scholar]
  4. Clark H. F., Soriano E. A. 1974; Fish rhabdovirus replication in non-piscine cell culture: new system for the study of rhabdovirus cell interaction in which the virus and cell have dilferent temperature optima. Infection and Immunity 10:180–188
    [Google Scholar]
  5. Clerx J. P. M., Van Der Zeijst B. A. M., Horzinek M. C. 1975; Some physicochemical properties of pike fry rhabdovirus RNA. The Journal of General Virology 29:133–136
    [Google Scholar]
  6. Clerx J. P. M., Horzinek M. C. 1978; Comparative protein analysis of non-salmonid fish rhabdoviruses. The Journal of General Virology 40: (this part)
    [Google Scholar]
  7. Della-Porta A. J., Westaway E. G. 1978; A multi-hit model for the neutralization of animal viruses. The Journal of General Virology 38:1–19
    [Google Scholar]
  8. Fijan N., Petrinec Z., Sulimanovic D., Zwillenberg L. O. 1971; Isolation of the viral causative agent from the acute form of infectious dropsy of carp. Veterinarski archiv (Zagreb) 41:125–138
    [Google Scholar]
  9. Hill B. J., Underwood B. O., Smale C. J., Brown F. 1975; Physicochemical and serological characterization of five rhabdoviruses infecting fish. The Journal of General Virology 27:369–378
    [Google Scholar]
  10. Horzinek M. C. 1973; Comparative aspects of togaviruses. The Journal of General Virology 20:87–103
    [Google Scholar]
  11. Kinkelin P., De Galimard B., Bootsma R. 1973; Isolation and identification of the causative agent of ‘red disease’ of pike (Esox lucius L., 1766). Nature, London 241:465–467
    [Google Scholar]
  12. Kinkelin P., De Le Berre M., Lenoir G. 1974; Rhabdovirus des poissons. I. Propriétés in vitro du virus de la maladie rouge de l’alevin de brochet. Annales de Microbiologie (Institut Pasteur) 125A:93–111
    [Google Scholar]
  13. Kinkelin P., De Le Berre M. 1974; Rhabdovirus des poissons. II. Propriétés in vitro du virus de la virémie printaniére de la carpe. Annales de Microbiologie (Institut Pasteur) 125A:113–124
    [Google Scholar]
  14. Mills B. J., Oldstone M. B. A., Cooper N. R. 1976; Complement-dependent lysis of vesicular stomatitis virus. Federation Proceedings 35:494
    [Google Scholar]
  15. Roy P., Clark H. F., Madore H. P., Bishop D. H. L. 1975; RNA polymerase associated with virions of pike fry rhabdovirus. Journal of Virology 15:338–347
    [Google Scholar]
  16. Zeijst B. A. M., Van Der & Bloemers H. P. J. 1976; Isokinetic glycerol and sucrose gradients for density gradient centrifugation. In The Handbook of Biochemistry 3rd edition. Physical and Chemical Data, vol 1 pp 426–519 Edited by Fasman G. D. Cleveland, Ohio, U.S.A.: The Chemical Rubber Company;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-40-2-297
Loading
/content/journal/jgv/10.1099/0022-1317-40-2-297
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error