1887

Abstract

SUMMARY

The antigen-antibody neutralization of filamentous phage Xf showed first-order kinetics within the first 10 minutes. Failure to reverse the neutralization reaction by dilution indicated that a stable combination might be formed between phage and antibody.

Antigenicity of Xf was analysed by agar double diffusion and three distinct precipitin lines were observed. For identifying these three antigenic components, Xf particles were dissolved and separated into A-protein and B-protein. The results of agar double diffusion showed that one precipitin line corresponds to the A-protein and the other two precipitin lines are determined by the B-protein.

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/content/journal/jgv/10.1099/0022-1317-40-1-253
1978-07-01
2024-04-25
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References

  1. Bowman B. U., Patnode R. A. 1964; Neutralization of bacteriophage ϕ X174 by specific antiserum. Journal of Immunology 92:507–514
    [Google Scholar]
  2. Kuo T. T., Huang T. C., Chow T. Y. 1969; A filamentous bacteriophage from Xanthomonas oryzae. Virology 39:548–555
    [Google Scholar]
  3. Marvin D. A., Hohn B. 1969; Filamentous bacterial viruses. Bacteriological Review 33:172–209
    [Google Scholar]
  4. Marvin D. A., Wiseman R. L., Wachtel E. J. 1974; Filamentous bacterial viruses. XI. Molecular architecture of the class II (pfl, Xf) virion. Journal of Molecular Biology 82:121–138
    [Google Scholar]
  5. Ouchterlony Ö. 1958; Diffusion-in-gel methods for immunological analysis. In Progress in Allergy 5:1–78
    [Google Scholar]
  6. Rolfe V., Sinsheimer R. L. 1965; Antigens of bacteriophage ϕ X174. Journal of Immunology 94:18–21
    [Google Scholar]
  7. Rowlands D. T. 1967; Precipitation and neutralization of bacteriophage f2 by rabbit antibodies. Journal of Immunology 98:958–964
    [Google Scholar]
  8. Witte O. N., Slobin L. I. 1972; Neutralization of haptenated bacteriophage f2 anti-hapten antibodies: direct evidence for a critical site. Journal of Immunology 108:927–936
    [Google Scholar]
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