1887

Abstract

SUMMARY

Direct plaque formation with representative strains of hog cholera virus (HCV) has been obtained using several pig kidney cell lines under agar overlay. HCV-infected cells appear as hazy plaques when viewed against an indirect light source, and as white plaques after neutral red staining. HCV assay by direct plaque procedure is rapid and convenient and gives infectivity titres identical to the fluorescent focus assay technique.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-40-1-225
1978-07-01
2022-01-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/40/1/JV0400010225.html?itemId=/content/journal/jgv/10.1099/0022-1317-40-1-225&mimeType=html&fmt=ahah

References

  1. Allison A. 1967 In Perspectives in Virology vol 2 pp 29–61 Edited by Pollard M. New York, London: Academic Press;
    [Google Scholar]
  2. Aynaud J. M. 1968; Ѐtude de la multiplication en cycle unique d’un clone de virus de la peste porcine classique au moyen de l’immunofluorescence. Compte rendus de I’Academie des Sciences 266:535–537
    [Google Scholar]
  3. Carbrey E. A., Stewart W. C., Kresse J. I., Lee L. R. 1965; Technical aspects of tissue culture fluorescent antibody technique. 96th Annual Proceedings of United States Livestock Sanitary Association487–500
    [Google Scholar]
  4. Cooper P. D. 1967 In Methods in Virology III6, 243–312 Edited by Maramorosch K., Koprowski H. New York, London: Academic Press;
    [Google Scholar]
  5. Corthier G., Aynaud J. M., Galicher C., Gelfi J. 1974; Activite antigenique comparee de deux togavirus: le virus de la peste porcine et le virus de la maladie des muqueuses. Annales de Recherches Vѐtѐrinaires 5:373–393
    [Google Scholar]
  6. Fukusho A., Ogawa N., Yamamoto H., Sawada M., Sazawa H. 1976; Reverse plaque formation by hog cholera virus of the GPE strains inducing heterologous interference. Infection and Immunity 14:332–336
    [Google Scholar]
  7. Kumagaϊ T., Shimizu T., Ikeda S., Matumoto M. 1961; A new in vitro method (END) for detection and measurement of hog cholera virus and its antibody by means of effect of HC virus on Newcastle disease virus in swine tissue cultures. Journal of Immunology 87:245–256
    [Google Scholar]
  8. Laude H. 1977; Improved method for the purification of hog cholera virus grown in tissue culture. Archives of Virology 54:41–51
    [Google Scholar]
  9. Laude H. 1978; Virus de la peste porcine classique: interference avec le VSV et titrage par le procѐdѐ des plages inverses. Archives of Virology 56:273–277
    [Google Scholar]
  10. Van Bekkum J. G., Barteling S. J. 1970; Plaque production by hog cholera virus. Archiv fur die gesamte Virusforschung 32:185–200
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-40-1-225
Loading
/content/journal/jgv/10.1099/0022-1317-40-1-225
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error