1887

Abstract

SUMMARY

It has been found that 1000-fold more bovine rotavirus is obtained when trypsin is incorporated in the maintenance medium and allowed to remain throughout the growth cycle. This holds true for primary calf kidney (CK) cells and also for several continuous and semi-continuous cell lines. In the presence of trypsin it has been possible to pass the virus serially on continuous cell lines seven times. Concentrations of 1 to 10 µg/ml of trypsin are found to be effective. Preliminary results suggest that the same technique will be effective for the propagation of human rotavirus.

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1978-07-01
2024-05-26
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References

  1. Appleyard G., Maber H. B. 1974; Plaque formation by influenza viruses in the presence of trypsin. Journal of General Virology 25:351–357
    [Google Scholar]
  2. Banatvala J. E., Totterdell B., Chrystie I. L., Woode G. N. 1975; In-vitro detection of human rotaviruses. Lancet ii:821
    [Google Scholar]
  3. Babiuk L. A., Mohammed K., Spence L., Fauvel M., Petro R. 1977; Rotavirus isolation and cultivation in the presence of trypsin. Journal of Clinical Microbiology 6:610–617
    [Google Scholar]
  4. Bridger J. C., Woode G. N. 1976; Characterization of two particle types of calf rotavirus. Journal of General Virology 31:245–250
    [Google Scholar]
  5. Carney D. H., Cunningham D. D. 1977; Initiation of chick cell division by trypsin action at the cell surface. Nature, London 268:602–606
    [Google Scholar]
  6. Flewett T. H., Bryden A. S., Davies H., Woode G. N., Bridger J. C., Derrick J. M. 1974; Relation between viruses from acute gastroenteritis of children and newborn calves. Lancet ii:61–63
    [Google Scholar]
  7. Holmes I. H., Ruck B. J., Bishop R. F., Davidson G. P. 1975; Infantile enteritis viruses: morphogenesis and morphology. Journal of Virology 16:937–943
    [Google Scholar]
  8. Isaacs A., Lindenmann J., Valentine R. C. 1957; Virus interference. II. Some properties of interferon. Proceedings of the Royal Society of Medicine B147:268–273
    [Google Scholar]
  9. Kapikian A. Z., Kim H. W., Wyatt R. G., Rodriguez W. J., Ross S., Cline W. L., Parrott R. H., Chanock R. M. 1974; Reovirus-like agent in stools: association with infantile diarrhoea and development of serologic tests. Science 185:1049–1053
    [Google Scholar]
  10. Klenk H.-D., Rott R., Orlich M., Blődorn J. 1975; Activation of influenza A viruses by trypsin treatment. Virology 68:426–439
    [Google Scholar]
  11. Lazarowitz S. G., Choppin P. W. 1975; Enhancement of the infectivity of influenza A and B viruses by proteolytic cleavage of the haemagglutinin polypeptide. Virology 68:440–454
    [Google Scholar]
  12. Matsuno S., Inouye S., Kono R. 1977; Plaque assay of neonatal calf diarrhoea and the neutralising antibody in human sera. Journal of Clinical Microbiology 5:1–4
    [Google Scholar]
  13. Rees D. A., Lloyd C. W., Thom D. 1977; Control of grip and stick in cell adhesion through lateral relationships of membrane glycoproteins. Nature, London 267:124–128
    [Google Scholar]
  14. Spendlove R. S., Mclain M. E., Lennette E. M. 1970; Enhancement of reovirus infectivity by extracellular removal or alteration of the virus capsid by proteolytic enzymes. Journal of General Virology 8:83–94
    [Google Scholar]
  15. Spendlove R. S., Schaffer F. L. 1965; Enzymatic enhancement of infectivity of reovirus. Journal of Bacteriology 89:597–602
    [Google Scholar]
  16. Theil K. W., Bohl E. H., Agnes A. G. 1977; Cell culture propagation of porcine rotavirus (reovirus-like agent). American Journal of Veterinary Research 38:1765–1768
    [Google Scholar]
  17. Thouless M. E., Bryden A. S., Flewett T. H., Woode G. N., Bridger J. C., Snodgrass D. R., Herring J. A. 1977; Serological relationships between rotaviruses from different species as studied by complement fixation and neutralization. Achives of Virology 53:287–294
    [Google Scholar]
  18. Totterdell B. M., Chrystie I. L., Banatvala J. E. 1976; Rotavirus infections in a maternity unit. Archives of Disease in Childhood 51:924–928
    [Google Scholar]
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