1887

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Volume 4, Issue 4

Restriction of a Transducing Bacteriophage in a Strain of Free

Abstract

Summary

Phage 34.13 adsorbs well to strain but does not form plaques on it. The DNA of the phage is severely degraded in strain . Phage which does emerge plates on strain 13 but not on . The phenotype of strain is rm. Restriction by stationary phase organisms is weaker than in early log. phase cells. The acceptor ability of for a - plasmid from strain 13 is less than that of strain 13. Spheroplasts of 13 plate 34.13 DNA with an efficiency of 10. The efficiency on strain spheroplasts is <10. The transduction rate of markers by 34.13 into is only reduced to about 10 of the rate into strain 13. Transductants are non-lysogenic for the phage, are stable and may be retransduced. Small doses of ultraviolet radiation do not increase the transduction rate. This is interpreted to mean that 34.13 transduces strain by integration of the bacterial exogenote, which like 34.13 DNA and possibly strain 13 - DNA, is degraded in strain mutants of strain were isolated on which 34.13 has an e.o.p. of 5 × 10 and into which 34.13 transduces markers at the same rate as into strain 13. Phage 34 mutants plate on strain . A strain -induced host specificity was discovered which must be carried by 34 for it to form plaques on . Strain and its mutants are lysogenic for 13 and produce a phage tail-like bacteriocin but neither of these factors account for the restricting and modifying properties of the strains. Phages 34.13 and 34−1. do not affect one another in mixed infections of strain .

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© Journal of General Virology 1969
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1969-06-01
2023-12-03
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Restriction of a Transducing Bacteriophage in a Strain of Proteus mirabilis
J. Gen. Virol. 4, 593 (1969); https://doi.org/10.1099/0022-1317-4-4-593
/content/journal/jgv/10.1099/0022-1317-4-4-593
/content/journal/jgv/10.1099/0022-1317-4-4-593
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