1887

Abstract

Summary

Bacteriophage PL25 was purified by centrifugation in caesium chloride. It has ° = 485; ° = 0.68 × 10 cm./sec. and = 54.3 × 10. An equation was derived relating ° and molecular weight of bacteriophages.

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/content/journal/jgv/10.1099/0022-1317-4-4-577
1969-06-01
2024-04-18
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References

  1. Chervenka C. H. 1966; Use of combined schlieren and interference optics for the determination of molecular weights from sedimentation equilibrium data. Analyt. Chem 38:356
    [Google Scholar]
  2. Coetzee J. N. 1963; Lysogeny in Providence strains and the host-range of Providence bacteriophages. Nature, Lond 197:515
    [Google Scholar]
  3. Coetzee J. N., Sacks T. G. 1960; Transduction of streptomycin resistance in Proteus mirabilis . J. gen. Microbiol 23:445
    [Google Scholar]
  4. Cummings D. J., Kozloff L. M. 1960; Biophysical properties of bacteriophages T2. Biochim. biophys. Acta 44:445
    [Google Scholar]
  5. Davison P. F., Freifelder D. 1962; The physical properties of T7 bacteriophage. J. molec. Biol 5:635
    [Google Scholar]
  6. Dyson R. D., Van Holde K. E. 1967; An investigation of Bacteriophage lambda, its protein ghosts and subunits. Virology 33:559
    [Google Scholar]
  7. Goldwasser E., Putnam F. 1952; Physicochemical properties of T6 bacteriophage. J. biol. Chem 190:61
    [Google Scholar]
  8. Josse J., Eigner J. 1966; Physical properties of deoxyribonucleic acid. A. Rev. Biochem 35:789
    [Google Scholar]
  9. Kupila S., Bryan A. M., Stern H. 1961; Extractability of DNA and its determination in tissues of higher plants. Plant Physiol 36:212
    [Google Scholar]
  10. Marvin D. A., Hoffmann-Berling H. 1963; A fibrous DNA phage (fd) and a spherical phage (fr) specific for male strains of E. coli . Z. Naturforsch 186:884
    [Google Scholar]
  11. Möller W. J. 1964; Determination of diffusion coefficients and molecular weights of ribonucleic acids and viruses. Proc. natn. Acad. Sci. U,. S,. A 51:501
    [Google Scholar]
  12. Mommaerts W. F. H. M., Aldrich B. B. 1958; Determination of the molecular weight of Myosin. Interference-optical measurements during the approach to ultracentrifugal sedimentation and diffusion equilibrium. Biochim. biophys. Acta 28:627
    [Google Scholar]
  13. Saunders G. F., Campbell L. L. 1966; Characterization of thermophilic bacteriophage for Bacillus stearothermophilus . J. Bad 91:340
    [Google Scholar]
  14. Schachman H. K. 1957; Ultracentrifugation, diffusion and viscometry. Methods in Enzymology vol. Lv 32 Eds Colowick S.P., Kaplan N.O. New York: Academic Press;
    [Google Scholar]
  15. Schito G. C., Rialdi G., Pesce A. 1966; Biophysical properties of N4 coliphage. Biochim. biophys. Acta 129:482
    [Google Scholar]
  16. Sinsheimer R. L. 1959; Purification and properties of bacteriophages x-174. J. molec. Biol 1:37
    [Google Scholar]
  17. Strauss J. H., Sinsheimer R. L. 1963; Purifications and properties of bacteriophage MS 2 and of its ribonucleic acid. J. molec. Biol 7:43
    [Google Scholar]
  18. Svedberg T., Pedersen K. O. 1940 The Ultracentrifuge Oxford University Press;
    [Google Scholar]
  19. Swanby L. S. 1959; Some biophysical properties of the T 3 bacteriophage of Escherichia coli . Diss. Abstr 20:1567
    [Google Scholar]
  20. Taylor N. W., Epstein H. T., Lauffer M. A. 1955; Particle weight, hydration and shape of the T2 bacteriophage of Escherichia coli.. J. Am. chem. Soc. 77:1270
    [Google Scholar]
  21. Van Holde K. E. 1960; A modification of Fujita’s method for the calculation of diffusion coefficients from boundary spreading in the ultracentrifuge. J. phys. Chem., Ithaca 641582
    [Google Scholar]
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