1887

Abstract

Summary

In chick fibroblast cell cultures infected with Newcastle disease virus an RNA-dependent RNA nucleotidyl transferase (RNA polymerase) has been detected which was not found in non-infected cells. The enzyme mainly synthesizes RNA complementary to viral RNA as shown by nearest neighbour analysis and hybridization experiments. In contrast to the influenza virus-induced enzyme the Newcastle disease virus enzyme cannot be inhibited by dextran sulphate. Its synthesis is not inhibited by actinomycin. It is first detectable 3 hr after infection and it has reached its highest activity at 5 hr after infection.

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/content/journal/jgv/10.1099/0022-1317-4-4-565
1969-06-01
2022-01-26
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