1887

Abstract

Summary

Twenty-six strains of foot-and-mouth disease virus have been studied by sucrose gradient zone electrophoresis and a broad range of mobility is evident. It has been established that the electrophoretic mobilities of strains are a unique heritable property of the virion. No covariation is evident with several and characteristics of these strains but there is a relationship between mobility and immunological type. This relationship is unconnected with serological specificity and probably reflects the phylogenetic divergence of the seven immunological types of foot-and-mouth disease virus.

Two types of stable electrophoretic mutant have been isolated by serial selection, one of which, however, exhibits phenotypic instability. The other is stable but can be shown to be at a selective disadvantage in competition with the normal virus.

It has not been possible to demonstrate phenotypic mixing in progeny from cells infected with electrophoretically different strains or mutants. The biological implications of this are discussed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-4-4-541
1969-06-01
2023-02-08
Loading full text...

Full text loading...

/deliver/fulltext/jgv/4/4/JV0040040541.html?itemId=/content/journal/jgv/10.1099/0022-1317-4-4-541&mimeType=html&fmt=ahah

References

  1. Ahl R. 1967; Genommaskierung beim Maul-und Klauenseuche Virus und das Verhalten von genommaskierten Virus bei der Thermo-Inaktivierung. Arch. ges. Virusforsch 21:217
    [Google Scholar]
  2. Bachrach H. L. 1961; Thermal degradation of foot-and-mouth disease virus into infectious ribonucleic acid. Proc. Soc. exp. Biol. Med 107:610
    [Google Scholar]
  3. Breeze D. C., Thorne H. V. 1966; Electrophoretic separation of two plaque type variants of encephalomyocarditis virus. Nature, LoNd 210:1080
    [Google Scholar]
  4. Brooksby J. B. 1952; The technique of complement fixation in foot- and-mouth disease research. ARC Rep Ser. no 12 London: H.M.S.O;
    [Google Scholar]
  5. Brown F., Cartwright B. 1963; Purification of radioactive foot-and-mouth disease virus. Nature, Lond 199:1168
    [Google Scholar]
  6. Castro M. P. D. 1964; Comportamento do virus aftoso em cultura de celulas suinas IB-RS-2. Archos Biol., S Paulo 31:63
    [Google Scholar]
  7. Cords C. E., Holland J. J. 1964; Alteration of the species and tissue specificity of poliovirus by enclosure of its RNA within the protein capsid of Coxsackie BI virus. Virology 24:292
    [Google Scholar]
  8. Dubes G. R., Tolbert O. 1961; Transfer of nongenetic thermostability from genetically thermostable to genetically thermolabile poliovirus. Arch. ges. Virusforsch 11:356
    [Google Scholar]
  9. Heidelberger C. 1965; Fluorinated pyrimidines. In Progress in Nucleic Acid Research and Molecular Biology 4:2
    [Google Scholar]
  10. Hirtz J. 1955; Electrophoretic properties of foot-and-mouth disease virus. Arch. ges. Virusforsch 6:124
    [Google Scholar]
  11. Hobom G., Braunitzer G. 1967a; Virusproteine. I. Isolierung von Mutanten des Phagen fd mit proteinchemischen Methoden. Hoppe-Seyler’s Z. physiol. Chem 348:783
    [Google Scholar]
  12. Hobom G., Braunitzer G. 1967b; Virusproteine. II. Untersuchung polycyclischer aromatischer Amine auf ihre mutagene Wirkung am Phagen fd. Hoppe-Seyler’s Z. physiol. Chem 348:797
    [Google Scholar]
  13. Itoh H., Melnick J. L. 1959; Double infections of single cells with Echo 7 and Coxsackie A9 viruses. J. exp. Med 109:393
    [Google Scholar]
  14. Ledinko N., Hirst G. K. 1961; Mixed infection of HeLa cells with poliovirus types I and 2. Virology 14:207
    [Google Scholar]
  15. Macpherson I. A., Stoker M. G. P. 1962; Polyoma transformation of hamster cell clones–An investigation of genetic factors affecting cell competence. Virology 16:147
    [Google Scholar]
  16. Matheka H. D., Geiss E. 1963; Das Verhalten tierischer Virusarten in der Tiselius-Elektrophorese. Zentbl. Bakt. ParasitKde (Abst. 1) 191:244
    [Google Scholar]
  17. Matheka H. D., Geiss E. 1965a; Die Bestimmung der Wanderungsgeschwindigkeit tierischer Virusarten im Dichtergradienten der trägerfreien Zonenelektrophorese. I. Mitteilung: Zur Methodik und Theorie der trägerfreien Zonenelektrophorese im GlukoseDochtergradienten. Arch. ges. Virusforsch 15:301
    [Google Scholar]
  18. Matheka H. D., Geiss E. 1965b; Die Bestimmung der Wanderungsgeschwindigkeit tierischer Virusarten im Dichtergradienten der trägerfreien Zonenelektrophorese. II. Mitteilung: Ergebnisse mit Influenzaund Newcastle-Disease-Virus sowie den Viren der Teschener Krankheit (Schweinelahmung) und der Maulund Klauenseuche (Typ A, C und O). Arch. ges. Virusforsch 15:327
    [Google Scholar]
  19. Matheka H. D., Bachrach H. L., Trautman R. 1966; Highly purified FMDV. optical and biological measurements during zone electrophoresis in a glucose density gradient. Z. Naturf 216:774
    [Google Scholar]
  20. Matthaeus W. 1966; Die Autoradiografie als Hilfsmittel zur Bestimmung der elektrophoretischen Beweglichkeit von Kulturvirus und zum Machweis des Reinheitsgrades von Viruspräparaten. Zentbl. Vet Med 13:782
    [Google Scholar]
  21. Munyon W., Salzman N. P. 1962; The incorporation of 5-fluorouracil into poliovirus. Virology 18:95
    [Google Scholar]
  22. Platt H. 1956; A study of the pathological changes produced in young mice by the virus of foot- and-mouth disease. J. path. bact 72:299
    [Google Scholar]
  23. Polson A., Decks D. 1962; Zone electrophoresis of enteroviruses. J. Hyg., Camb 60:217
    [Google Scholar]
  24. Poppe K., Busch G. 1930; Physikalische und chemische Untersuchungen am Virus der Maul-und Klauenseuche. I. Isoelektrische Punkt und Adsorption. Z. Immunoforsch. exp. Ther 68:510
    [Google Scholar]
  25. Pringle C. R. 1964; Inhibition of multiplication of foot-and-mouth disease virus by guanidine hydrochloride. Nature, Lond 204:1012
    [Google Scholar]
  26. Pringle C. R. 1968; Recombination between conditional lethal mutants within a strain of foot- and-mouth disease virus. J. gen. Virol 2:199
    [Google Scholar]
  27. Pringle C. R., Slade W. R. 1968; The originof hybrid variants derived from subtype strains of foot- and-mouth disease virus. J. gen. Virol 2:319
    [Google Scholar]
  28. Pringle C. R., Cartwright L. N. 1969; An inhibitor of reovirus in sheep serum. J. med. Microbiol. (in the Press)
    [Google Scholar]
  29. Sprunt K., Redman W. M., Alexander H. E. 1959; Combination of antigenic traits of type 1 and type 2 poliovirus. J. Immunol 82:232
    [Google Scholar]
  30. Sprunt K., Mountain I. M., Redman W. M., Alexander H. E. 1955; Production of poliovirus with combined antigenic characteristics of type I and type II. Virology 1:236
    [Google Scholar]
  31. Thorne H. V. 1963; Electrophoretic study of the interaction of radioactive poliovirus with components of cultured cells. J. Bact 85:217
    [Google Scholar]
  32. Thorne H. V., House W., Kisch A. L. 1965; Electrophoretic properties and purification of large and small plaque-forming strains of polyoma virus. Virology 27:37
    [Google Scholar]
  33. Wild T. F., Brown F. 1967; Nature of the inactivating action of trypsin on foot-and-mouth disease virus. J. gen. Virol 1:247
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-4-4-541
Loading
/content/journal/jgv/10.1099/0022-1317-4-4-541
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error