Localization of Virus Antigens by Enzyme-labelled Antibodies Free

Abstract

Summary

Three enzymes—horseradish peroxidase, alkaline phosphatase, and glucose oxidase—were used as markers in antibody conjugates employed for the detection in the light microscope of either or both T antigens and structural antigens of three viruses: SV40, adenovirus 12 and rat K virus, in infected cell cultures. The specificity of reaction and localization of the antigens was the same with each enzyme and identical with those revealed by fluorochromeconjugates. By using enzymes with reaction products of different colours, two antigens were revealed simultaneously in a single preparation.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-4-4-465
1969-06-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/4/4/JV0040040465.html?itemId=/content/journal/jgv/10.1099/0022-1317-4-4-465&mimeType=html&fmt=ahah

References

  1. Avrameas S. 1969; Coupling of enzymes to proteins with glutaraldehyde Use of the conjugates for the detection of antigens and antibodies.. Immunochemistry 6:43
    [Google Scholar]
  2. Avrameas S., Ternynck T. 1969; The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents. Immunochemistry 6:53
    [Google Scholar]
  3. Avrameas S., Uriel J. 1966; Methode de marquage d’antigenes et d’anticorps avec des enzymes et son application en immunodiffusion. C. r. Séanc. Acad. Sci., Paris 262:2543
    [Google Scholar]
  4. Bernier G. H., Cebra J. J. 1964; Polypeptide chains of human γ globulin: cellular localization by fluorescent antibody.. Science, N. Y 144:1590
    [Google Scholar]
  5. Burstone M. S. 1962 In Enzyme Histochemistry pp 275–6 New York: Academic Press;
    [Google Scholar]
  6. Cassingena R., Tournier P. 1965; Interaction adenovirus 12—cellules de Mesocricetus auratus.. Ann. Inst. Pasteur 108:277
    [Google Scholar]
  7. Coons A. H., Leduc E., Conolly J. M. 1955; Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit.. J. exp. Med 102:49
    [Google Scholar]
  8. Eagle H. 1955; Propagation in a fluid medium of a human epidermoid carcinoma strain kb. Proc. Soc. exp. Biol. Med 89:362
    [Google Scholar]
  9. Eddy B. E., Borman G. S., Grubbs G. E., Young R. D. 1962; Identification of the oncogenic substance in Rhesus monkey kidney cell cultures as Simian Virus 40. Virology 17:65
    [Google Scholar]
  10. Graham R. C., Karnovsky M. 1966; The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.. J. Histochem. Cytochem 14:291
    [Google Scholar]
  11. Granboulan N., Tournier P., Wicker R., Bernhard W. 1963; An electron microscope study of the development of SV40 virus. J. Cell Biol 17:423
    [Google Scholar]
  12. Leduc E., Avrameas S., Boltteille M. 1967; Ultrastructural study of antibody production in differentiating plasma cells of rabbit spleen. J. Cell Biol 35:79 A.
    [Google Scholar]
  13. Nakane P. K., Pierce G. B. 1966; Enzyme-labelled antibodies: preparation and application for the localization of antigens.. J. Histochem. Cytochem 14:929
    [Google Scholar]
  14. Nakane P. K., Pierce G. B. Jun 1967; Enzyme-labelled antibodies for the light and electron microscopic localization of tissue antigens. J. Cell Biol 33:367
    [Google Scholar]
  15. Pearse A. G. E. 1960 Histochemistry, theoretical and applied London: J. and A. Churchill.:
    [Google Scholar]
  16. Pope J. H., Rowe W. P. 1964a; Detection of specific antigen in SV 40-transformed cells by immunofluorescence. J. exp. Med 120:121
    [Google Scholar]
  17. Pope J. H., Rowe W. P. 1964b; Immunofluorescent studies of adenovirus 12 tumors and cells transformed or infected by adenovirus.. J. exp. Med 120:577
    [Google Scholar]
  18. Ram J. S., Nakane P. K., Rawlinson D. G. 1966; Enzyme-labelled antibodies forultrastructural studies. Fedn Proc. Fedn Am. Socs exp. Biol 25:732
    [Google Scholar]
  19. Rapp F., Kitahara T. S., Butel J. S., Melnick J. L. 1964; Synthesis of SV 40 tumor antigen during replication of simian papovavirus (SV 40).. Proc. natn. Acad. Sci. U,. S,. A 52:1138
    [Google Scholar]
  20. Scott G., Avrameas S., Bernhard W. 1968; Étude au microscope électronique de la formation d’anticorps à l’aide de phosphatase alcaline utilisée comme antigfène. C. r. Seanc. Acad. Sci., Paris 266:746
    [Google Scholar]
  21. Tournier P., Cassingena R., Wicker R., Coppey J., Suarez H. 1967; Étude du mécanisme de l’induction chez des cellules de Hamster syrien transformées par le virus SV 40.1. Propriety d’une lignte cellulaire clonale. Int. J. Cancer 2:117
    [Google Scholar]
  22. Trentin J. J., Yabe Y., Taylor G. 1962; The quest for human cancer viruses. Science, N.Y 137:835
    [Google Scholar]
  23. Vasquez C., Brailovsky C. 1965; Purification and fine structure of Kilham’s rat virus. Exp. molec. Pathol 4:130
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-4-4-465
Loading
/content/journal/jgv/10.1099/0022-1317-4-4-465
Loading

Data & Media loading...

Most cited Most Cited RSS feed