Sequential cytological developments in primary dog kidney cells infected with a canine laryngotracheitis virus were studied by examining thin sections with the electron microscope. Early changes in the infected cells were enlargement of the nucleolus and the appearance of heterogeneous dark-staining granules in the nucleus. These granules developed into ‘initial bodies’ which were shown to contain DNA. The initial bodies increased in size to form ‘rings’ or more correctly spheres containing less densely staining central cores. The cores disappeared as the inclusions increased in size. Virus particles were occasionally seen at 14 hr and by 16 hr many of the cells contained virus. As the virus particles increased in number they usually aggregated and migrated towards the periphery of the nucleus. Release of the virus into the cytoplasm appeared to be by the formation of protrusions of the nucleus and the pinching off of membrane-bound virus aggregates.


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