RT Journal Article SR Electronic(1) A1 Kassanis, B. A1 Lebeurier, GenevièveYR 1969 T1 The Behaviour of Tomato Bushy Stunt Virus and Bromegrass Mosaic Virus at Different Temperatures in vivo and in vitro JF Journal of General Virology, VO 4 IS 3 SP 385 OP 395 DO https://doi.org/10.1099/0022-1317-4-3-385 PB Microbiology Society, SN 1465-2099, AB Summary The multiplication and inactivation of tomato bushy stunt virus (TBSV) and of bromegrass mosaic virus (BGMV), both of which have spherical particles, differed greatly at different temperatures. TBSV was inactivated in and disappeared from infected plants at 36°; BGMV maintained its content and virus produced at 36° was as infective as that produced at 20°. Infectivity of TBSV was lost in vivo or in vitro without any apparent change in the physical properties of the particles or of the nucleic acid. BGMV was unaffected by heating in vitro for an hour at 45° at pH 5.8, but at pH 7.0 lost its infectivity within an hour at 36°. Even at 20° the particles were unstable and, in the presence of pancreatic ribonuclease, some but not all particles disintegrated to produce two new components. BGMV from plants infected for 1 week was, weight for weight, more infective than virus from plants infected for 3 weeks, and contained proportionally more 27 S nucleic acid than non-infective 22 S or 14S, RNAs. When virus was heated at 31° in buffer pH 6.8 the amounts of S27 and S22 nucleic acid diminished, but amounts of 14S nucleic acid diminished only at higher temperatures. Thus inactivation of BGMV, but not of TBSV, may reflect breaking of the nucleic acid., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-4-3-385