The multiplication and inactivation of tomato bushy stunt virus (TBSV) and of bromegrass mosaic virus (BGMV), both of which have spherical particles, differed greatly at different temperatures. TBSV was inactivated in and disappeared from infected plants at 36°; BGMV maintained its content and virus produced at 36° was as infective as that produced at 20°. Infectivity of TBSV was lost or without any apparent change in the physical properties of the particles or of the nucleic acid.

BGMV was unaffected by heating for an hour at 45° at pH 5.8, but at pH 7.0 lost its infectivity within an hour at 36°. Even at 20° the particles were unstable and, in the presence of pancreatic ribonuclease, some but not all particles disintegrated to produce two new components. BGMV from plants infected for 1 week was, weight for weight, more infective than virus from plants infected for 3 weeks, and contained proportionally more 27 nucleic acid than non-infective 22 or 14, RNAs. When virus was heated at 31° in buffer pH 6.8 the amounts of 27 and 22 nucleic acid diminished, but amounts of 14 nucleic acid diminished only at higher temperatures. Thus inactivation of BGMV, but not of TBSV, may reflect breaking of the nucleic acid.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error