1887

Abstract

Summary

The multiplication and inactivation of tomato bushy stunt virus (TBSV) and of bromegrass mosaic virus (BGMV), both of which have spherical particles, differed greatly at different temperatures. TBSV was inactivated in and disappeared from infected plants at 36°; BGMV maintained its content and virus produced at 36° was as infective as that produced at 20°. Infectivity of TBSV was lost or without any apparent change in the physical properties of the particles or of the nucleic acid.

BGMV was unaffected by heating for an hour at 45° at pH 5.8, but at pH 7.0 lost its infectivity within an hour at 36°. Even at 20° the particles were unstable and, in the presence of pancreatic ribonuclease, some but not all particles disintegrated to produce two new components. BGMV from plants infected for 1 week was, weight for weight, more infective than virus from plants infected for 3 weeks, and contained proportionally more 27 nucleic acid than non-infective 22 or 14, RNAs. When virus was heated at 31° in buffer pH 6.8 the amounts of 27 and 22 nucleic acid diminished, but amounts of 14 nucleic acid diminished only at higher temperatures. Thus inactivation of BGMV, but not of TBSV, may reflect breaking of the nucleic acid.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-4-3-385
1969-04-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/4/3/JV0040030385.html?itemId=/content/journal/jgv/10.1099/0022-1317-4-3-385&mimeType=html&fmt=ahah

References

  1. Bancroft J. B., Hills G. J., Markham R. 1967; A study of the self-assembly process in a small spherical virus. Formation of organized structures from protein subunits in vitro . Virology 31:354
    [Google Scholar]
  2. Bockstahler L. E., Kaesberg P. 1962; The molecular weight and other biophysical properties of bromegrass mosaic virus. Biophys. J 2:1
    [Google Scholar]
  3. Bockstahler L. E., Kaesberg P. 1965a; Infectivity studies of bromegrass mosaic virus RNA. Virology 27:418
    [Google Scholar]
  4. Bockstahler L. E., Kaesberg P. 1965b; Isolation and properties of RNA from bromegrass mosaic virus. J. molec. Biol 13:127
    [Google Scholar]
  5. Chin R., Sill W. H. 1963; Factors affecting assay of bromegrass mosaic virus on Datura stramonium and Chenopodium hybridum. . Phytopathology 53:69
    [Google Scholar]
  6. Gillaspie A. G., Bancroft J. B. 1965; The rate of accumulation, specific infectivity, and electrophoretic characteristics of bean pod mottle virus in bean and soybean. Phytopathology 55:906
    [Google Scholar]
  7. Havránek P. 1967; The effect of inoculum concentration on virus multiplication in cucumber cotyledons. II. Cucumber mosaic and tobacco mosaic viruses. Acta virol. Prague 11:538
    [Google Scholar]
  8. Hitchborn J. H. 1956; The effect of temperature on infection with strains of cucumber mosaic virus. Ann. appl. Biol 44:590
    [Google Scholar]
  9. Hitchborn J. H. 1957; The effect of high temperature on the multiplication of two strains of tobacco ring spot virus. Virology 3:243
    [Google Scholar]
  10. Incardona N. L., Kaesberg P. 1964; A pH-induced structural change in bromegrass mosaic virus. Biophys. J 4:11
    [Google Scholar]
  11. Izadpanah K., Shepherd R. J. 1966; Purification and properties of the pea enation mosaic virus. Virology 28:463
    [Google Scholar]
  12. Kassanis B. 1952; Some effects of high temperatures on the susceptibility of plants to infection with viruses. Ann. appl. Biol 39:358
    [Google Scholar]
  13. Kassanis B. 1954; Heat-therapy of virus-infected plants. Ann. appl. Biol 41:470
    [Google Scholar]
  14. Kassanis B. 1957a; Some effects of varying temperature on the quality and quantity of tobacco mosaic virus in infected plants. Virology 4:187
    [Google Scholar]
  15. Kassanis B. 1957b; Effects of changing temperature on plant virus diseases. Adv. Virus Res 4:221
    [Google Scholar]
  16. Kassanis B., McCarthy D. 1967; The quality of virus as affected by the ambient temperature. J. gen. Virol 1:425
    [Google Scholar]
  17. Kodama T., Bancroft J. B. 1964; Some properties of infectious ribonucleic acid from broad bean mottle virus. Virology 22:23
    [Google Scholar]
  18. Kuhn C. W., Bancroft J. B. 1961; Concentration and specific infectivity changes of alfalfa mosaic virus during systemic infection. Virology 15:281
    [Google Scholar]
  19. Lebeurier G., Hirth L. 1966; Effect of elevated temperatures on the development of two strains of tobacco mosaic virus. Virology 29:385
    [Google Scholar]
  20. Luftig R. 1967; An accurate measurement of the catalase crystal period and its use as an internal marker for electron microscopy. J. Ultrastruct. Res 20:91
    [Google Scholar]
  21. Lyttleton J. W., Matthews R. E. F. 1958; Release of nucleic acid from turnip yellow mosaic virus under mild conditions. Virology 6:460
    [Google Scholar]
  22. Matthews R. E. F., Lyttleton J. W. 1959; Heat inactivation of turnip yellow mosaic virus in vivo . Virology 9:332
    [Google Scholar]
  23. Rochow W. F. 1959; Chenopodium hybridum as a local-lesion assay host for brome mosaic virus. Phytopathology 49:126
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-4-3-385
Loading
/content/journal/jgv/10.1099/0022-1317-4-3-385
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error