1887

Abstract

Summary

Crystals of intracellular foot-and-mouth disease virus, type A1, were reacted with ferritin conjugated to an A1 antibody, an O2 antibody, and normal γ-globulin as well as unconjugated ferritin. Specific tagging was demonstrated with both the full and ‘empty’ capsid crystals with type A1 virus and anti-A1 globulin. Anti-O2 ferritin-conjugated globulin, normal ferritin-conjugated γ-globulin and unconjugated ferritin did not attach to the A1 virus and showed only minimal reaction with tissue fragments and debris. Immunodiffusion in agar gel was used to demonstrate the specificity of the reagents.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-4-3-343
1969-04-01
2022-01-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/4/3/JV0040030343.html?itemId=/content/journal/jgv/10.1099/0022-1317-4-3-343&mimeType=html&fmt=ahah

References

  1. Breese S. S. Jun. 1968; Observations on complete and empty capsids of foot-and-mouth disease virus.. J. gen. Virol 2:465
    [Google Scholar]
  2. Breese S. S. Jun., Graves J. H. 1966; Electron microscopic observation of crystalline arrays of foot-and-mouth disease virus.. J. Bact 92:1835
    [Google Scholar]
  3. Breese S. S. Jun., Graves J. H. 1967; Crystalline arrays of foot-and mouth disease virus in tissue culture cells. Proc 25th Ann. Meet. Electron Microscope Society of America p. 102 Baton Rouge, La.: Claitor.;
    [Google Scholar]
  4. Cowan K. M., Graves J. H. 1966; A third antigenic component associated with foot-and-mouth disease virus. Virology 30:528
    [Google Scholar]
  5. Graves J. H., Cowan K. M., Trautman R. 1968; Immunochemical studies of foot-and-mouth disease virus. II. Characterization of RNA-free virus-like particles. Virology 34:269
    [Google Scholar]
  6. Luft J. H. 1961; Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol 9:404
    [Google Scholar]
  7. Millonig G. 1961 Abst. B26. 19th Ann. Meet Electron Microscope Society of America.. J. appl. Phys 32:1637
    [Google Scholar]
  8. Rifkind R. A., Hsu K. C., Morgan C. 1964; Immunochemical staining for electron microscopy. J. Histochem. Cytochem 12:131
    [Google Scholar]
  9. Singer S. J. 1959; Preparation of an electron dense antibody conjugate. Nature, Lond 183:1523
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-4-3-343
Loading
/content/journal/jgv/10.1099/0022-1317-4-3-343
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error