1887

Abstract

Summary

Following Newcastle disease virus infection, the synthesis of both cellular RNA and DNA in chick embryo fibroblast monolayers was inhibited. In such monolayers, Newcastle disease virus infection did not significantly reduce the content of cellular DNA within 10 hr, but did reduce the amount of cellular RNA by about 50% in that time. A part of the RNA was degraded into acid-soluble material and leaked out of the cells. Some fraction of the ribosomal RNA gave rise to small molecular weight fragments which sedimented near the transfer RNA region as determined by sucrose density gradient centrifugation. Evidence is presented indicating that virus-induced proteins, which were synthesized by 6 hr after infection, were involved in the RNA degradation process.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-4-2-245
1969-03-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/4/2/JV0040020245.html?itemId=/content/journal/jgv/10.1099/0022-1317-4-2-245&mimeType=html&fmt=ahah

References

  1. Allison A. C., Sandelin K. 1963; Activation of lysosomal enzymes in virus-infected cells and its possible relationship to cytopathic effects. J. exp. Med 117:879
    [Google Scholar]
  2. Flanagan J. F. 1966; Hydrolytic enzymes in KB cells infected with poliovirus and herpes simplex virus. J. Bact 91:789
    [Google Scholar]
  3. Hausen P., Verwoerd D. W. 1963; Studies on the multiplication of a member of the Columbia SK group (ME virus) in L cells, III. Alteration of RNA and protein synthetic patterns in virus-infected cells. Virology 21:617
    [Google Scholar]
  4. Kaplan A. S., Ben-Porat T. 1963; The pattern of viral and cellular DNA synthesis in pseudorabies virus-infected cells in the logarithmic phase of growth. Virology 19:205
    [Google Scholar]
  5. Salzman N. P., Lockart R. Z., Sebring E. D. 1959; Alterations in HeLa cell metabolism resulting from poliovirus infection. Virology 9:244
    [Google Scholar]
  6. Schmidt G., Thannhauser S. T. 1945; A method for determination of deoxyribonucleic acid, ribonucleic acid and phosphoprotein in animal tissue. J. biol. Chem 161:83
    [Google Scholar]
  7. Scherrer K., Darnell J. E. 1962; Sedimentation characteristics of rapidly labelled RNA from HeLa cells. Biochem Biophys. Res. Commun. 7:486
    [Google Scholar]
  8. Wiesner R., Reich G. A. E., Shafig A. 1965; Degradation of ribonucleic acid in mouse fibroblasts treated with actinomycin D. J. Cell Biol 27:47
    [Google Scholar]
  9. Wilson D. E. 1968; Inhibition of host-cell protein and RNA synthesis by Newcastle disease virus. J. Virol 2:1
    [Google Scholar]
  10. Wilson D. E., LoGerfo P. 1964; Inhibition of ribonucleic acid synthesis in Newcastle disease virus-infected cells by puromycin and 6-azauridine. J. Bact 88:1550
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-4-2-245
Loading
/content/journal/jgv/10.1099/0022-1317-4-2-245
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error