1887

Abstract

Summary

Centrifugation of post-ribosomal supernatant fluids of brain homogenates at high speed for a prolonged period yielded a transparent pellet material. Resuspension of this pellet followed by centrifugation on a caesium chloride gradient resulted in a sharp band at a density of 1.34 g./cm.. The band material sedimented as a single peak of approximately 5 in the analytical ultracentrifuge and had scrapie activity. Although the position of the band and its value are similar in normal and scrapie-affected mice there was an increased incorporation of [H]thymidine and [C]uridine-diphosphoglucose into the gradient fractions immediately above and into the band at a density of 1.3 to 1.34 g./cm. in scrapie-affected mice. Chromatography of the resuspended post-ribosomal pellet on Sepharose 4B yielded two peaks. In scrapie-affected mice, incorporation experiments showed an area between the two peaks with increased incorporation of [H]thymidine, [C]uridine-diphospho-glucose and [C]glucosamine. Experiments on spleen homogenates yielded comparable results.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-4-1-89
1969-01-01
2019-11-12
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-4-1-89
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error