Skip to content
1887

Journal of General Virology header logo Journal of General Virology

Volume 4, Issue 1

Ribonucleic Acid Nucleotidyl Transferase Induced in Chick Fibroblasts after Infection with an Influenza Virus No Access

Abstract

SUMMARY

A RNA-dependent RNA-nucleotidyl transferase (RNA-polymerase) has been demonstrated in chick fibroblasts infected with fowl plague virus. This enzyme was not found in uninfected cells. It needed Mg-ions and all four nucleoside triphosphates for its activity. Pretreatment with RNase or addition of RNase prevented the incorporation of [H]GTP into acid insoluble material. DNase had no effect. The enzyme’s activity was only slightly inhibited by actinomycin or proflavine. The incorporation of [H]GTP could be stimulated by RNA isolated from rat liver microsomes. It was strongly inhibited by small doses of polyvinyl sulphate or dextran sulphate, and by larger amounts of DNA and protamine sulphate. The enzyme was found in the microsomal and—at a lower activity—in the nuclear fraction of infected cells. enzymic activity could not be detected until 1 hr after infection; it then increased steadily with increasing time. The rate of synthesis, however, resembled that of viral RNA synthesis. The appearance of the enzyme could be inhibited by actinomycin, actidione, or -fluorophenylalanine. If actinomycin was added 2 hr after infection to the cells, viral RNA synthesis ceased completely at 6 hr after infection, in spite of the fact that large amounts of RNA polymerase could be then detected after homogenization.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1968
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-4-1-125
1969-01-01
2025-01-20
Loading full text...

Full text loading...

References

  1. Arlinghaus R. B., Polatnick J. 1967; Detergent-solubilized RNA polymerase from cells infected with foot-and-mouth disease virus. Science, N,. Y 158:1320
     [Google Scholar]
  2. Baltimore D., Franklin R. M. 1963; A new ribonucleic acid polymerase appearing after mengovirus infection of L cells. J. biol. Chem 238:3395
     [Google Scholar]
  3. Baltimore D., Eggers H. J., Franklin R. M., Tamm I. 1963; Poliovirus-induced RNA polymerase and the effects of virus-specific inhibitors on its production. Proc. natn. Acad,. Sci,. U.S.A. 49:843
     [Google Scholar]
  4. Barry R. D., Ives D. R., Cruickshank J. G. 1962; Participation of deoxyribonucleic acid in the multiplication of influenza virus. Nature, Lond 194:1139
     [Google Scholar]
  5. Borland R., Mahy B. W. J. 1968; Deoxyribonucleic acid-dependent ribonucleic acid polymerase activity in cells infected with influenza virus. J. Virol 2:33
     [Google Scholar]
  6. Glasky A. J., Holper J. C. 1964; Inhibition of ribonucleic acid synthetase from influenza and Newcastle disease virus-infected cells by various antiviral compounds. Antimicrobial Agents and Chemotherapy 1963: p. 688
    [Google Scholar]
  7. Ho P. P. K., Walters C. P. 1966; Influenza virus-induced ribonucleic acid nucleotidyl transferase and the effect of actinomycin D on its formation. Biochemistry 5:231
     [Google Scholar]
  8. Huppert J., Lacour F., Harel J., Harel L. 1966; High molecular weight RNA from avian myeloblastosis virus. Cancer Res 26:1561
     [Google Scholar]
  9. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. biol,. Chem 193:265
     [Google Scholar]
  10. Martin E. M., Sonnabend J. A. 1967; Ribonucleic acid polymerase catalyzing synthesis of double-stranded arbovirus ribonucleic acid. J. Virol 1:97
     [Google Scholar]
  11. Polatnick J., Arlinghaus R. B. 1967; Foot-and-mouth disease virus-induced ribonucleic acid polymerase in baby hamster kidney cells. Virology 31:601
     [Google Scholar]
  12. Pons M. 1967; Effect of actinomycin D on the replication of influenza virus and influenza virus RNA. Virology 33:150
     [Google Scholar]
  13. Rott R., Saber S., Scholtissek C. 1965; Effect on myxoviruses of mitomycin C, actinomycin D, and pretreatment of the host cell with ultraviolet light. Nature, Lond 205:1187
     [Google Scholar]
  14. Scherrer K., Darnell J. E. 1962; Sedimentation characteristics of rapidly labelled RNA from HeLa cells. Biochem. biophys,. Res,. Commun 7:486
     [Google Scholar]
  15. Scholtissek C. 1967; Nucleotide metabolism in tissue culture cells at low temperatures. I. Phosphorylation of nucleosides and deoxynucleosides in vivo . Biochim biophys.Acta 14s:228
     [Google Scholar]
  16. Scholtissek C., Rorr R. 1961; Zusammenhänge zwischen der Synthese von Ribonucleinsäure und Protein bei der Vermehrung eines Virus der Influenza-Gruppe (Virus der Klassischen Geflügelpest). Z,. Naturf 16b:663
     [Google Scholar]
  17. Scholtissek C., Rott R. 1964a; Behaviour of virus-specific activities in tissue cultures infected with myxoviruses after chemical changes of the viral ribonucleic acid. Virology 22:169
     [Google Scholar]
  18. Scholtissek C., Rott R. 1964b; Binding of proflavine to deoxyribonucleic acid and ribonucleic acid and its biological significance. Nature Lond 204:39
     [Google Scholar]
  19. Scholtissek C., Becht H., Drzeniek R. 1967; Biochemical studies on the cytopathic effect of influenza virus. J. gen,. Virol 1:219
     [Google Scholar]
  20. Scholtissek C., Rott R., Hausen P., Hausen H., Schafer W. 1962; Comparative studies of RNA and protein synthesis with a myxovirus and a small polyhedral virus. Cold Spring Harb. Symp.quant,. Biol 27:245
     [Google Scholar]
  21. Stakhanova V. M., Scholtissek C. 1967; Action of proflavine on RNA synthesis of Newcastle disease virus. J. gen,. Virol 1:571
     [Google Scholar]
  22. Wilson R. G., Bader J. P. 1965; Viral ribonucleic acid polymerase: chick embryo cells infected with vesicular stomatitis virus or Rous-associated virus. Biochim,. Biophys,. Acta 103:549
     [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-4-1-125
Loading
Ribonucleic Acid Nucleotidyl Transferase Induced in Chick Fibroblasts after Infection with an Influenza Virus
J. Gen. Virol. 4, 125 (1969); https://doi.org/10.1099/0022-1317-4-1-125
/content/journal/jgv/10.1099/0022-1317-4-1-125
/content/journal/jgv/10.1099/0022-1317-4-1-125
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error