Four of eight mycobacteriophages did not form plaques after they were exposed to chloroform. Phages sensitive to chloroform did not produce plaques when plated on media containing 1000 µg/ml of streptomycin sulphate. The same concentration of dihydrostreptomycin sulphate did not interfere with plaque formation. Mutants of Mycobacterium smegmatis resistant to each of the eight phages were isolated. Sensitivity or resistance to chloroform and streptomycin sulphate and phage resistant bacterial mutants may provide a basis for classifying the mycobacteriophages.
BowmanB. U.Jun,
NewmanH. A. I.,
MoritzJ. M.,
KoehlerR. M.1973; Properties of mycobacteriophage DS6A. II. Lipid composition. American Review of Respiratory Diseases 107:42–49
JonesW. D.Jun,
DavidH. L.1971; Inhibition by rifampin of mycobacteriophage D29 replication in its drug resistant host Mycobacterium smegmatis ATCC 607. American Review of Respiratory Diseases 103:618–624
JonesW. D.Jun,
DavidH. L.,
BeamR. E.1970; The occurrence of lipids in mycobacteria phage D29 propagated in Mycobacterium smegmatis ATCC 607. American Review of Respiratory Diseases 102:814–817
JonesW. D.Jun,
GreenbergJ.1977; Host modification and restriction with a mycobacteriophage isolated from a pseudolysogenic Mycobacterium chelonei. Journal of General Microbiology 99:389–395
JonesW. D.Jun,
WhiteA.1968; Lysogeny in mycobacteria. I. Conversion of colony morphology, nitrate reductase activity, and Tween 80 hydrolysis of Mycobacterium sp. ATCC 607 associated with lysogeny. Canadian Journal of Microbiology 14:551–555
SellersM. I.,
TokunagaR.1970; Inactivation of mycobacteriophages by lipid solvents. In Host-Virus Relationships in Mycobacterium, Nocardia and Actinomycetes pp 134–143 Edited by
JuhaszS. E.,
PlummerG.
Springfield, Illinois: Charles C. Thomas;