1887

Abstract

SUMMARY

Simple biochemical measurements have been shown to seriously overestimate the production of C-type particles by Chinese hamster ovary (CHO) cells treated with 5-bromodeoxyuridine. First, most particle-bound DNA polymerase activity released by induced cells was associated with particles which had a different density from C-type particles. Second, when labelled with radioactive uridine, induced CHO cells released small amounts of particle-bound radioactivity. Most of the radioactivity, however, was in DNA and did not sediment with the particle-bound polymerase. Thus, few particles which had RNA, an associated DNA polymerase, and the density typical of RNA tumour viruses were released by BrdUrd-induced CHO cells. In spite of this, some immature C-type forms were observed by electron microscopy in partially purified preparations of DNA polymerase-containing particles from induced CHO cells.

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1978-06-01
2024-12-14
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References

  1. Adams R. L. P. 1968; Incorporation of [5-3H]uridine into DNA. FEBS Letters 2:91–92
    [Google Scholar]
  2. Baltimore D. 1970; Viral RNA-dependent DNA polymerase. Nature, London 226:1209–1211
    [Google Scholar]
  3. Baltimore D., Smoler D. 1971; Primer requirement and template specificity of the DNA polymerase of RNA tumor viruses. Proceedings of the National Academy of Sciences of the United States of America 68:1507–1511
    [Google Scholar]
  4. Besmer P., Smotkin D., Haseltine W., Fan H., Wilson A. T., Paskind M., Weinberg R., Baltimore D. 1974; Mechanism of induction of RNA tumor viruses by halogenated pyrimidines. Cold Spring Harbor Symposia on Quantitative Biology 39:1103–1107
    [Google Scholar]
  5. Eckner R. J., Hettrick K. L. 1977; Defective Friend spleen focus-forming virus: interfering properties and isolation free from standard leukemia-inducing helper virus. Journal of Virology 24:383–396
    [Google Scholar]
  6. Gerard G. F. 1975; Poly (2′O-methylcytidylate) oligodeoxyguanylate, a template-primer for reverse transcriptase, is not utilized by HeLa cell 7 DNA polymerases. Biochemical and Biophysical Research Communications 63:706–711
    [Google Scholar]
  7. Gerard G. F., Rottman F., Green M. 1974; Poly (2′-O-methylcytidylate) oligodeoxyguanylate as a template for the ribonucleic acid directed deoxyribonucleic acid polymerase in ribonucleic acid tumor virus particles and a specific probe for the ribonucleic acid directed enzyme in transformed murine cells. Biochemistry 13:1632–1641
    [Google Scholar]
  8. Hsie A. W., Puck T. T. 1971; Morphological transformation of Chinese hamster cells by dibutyryl adenosine cyclic 3′: 5′-monophosphate and testosterone. Proceedings of the National Academy Sciences of the United States of America 68:358–361
    [Google Scholar]
  9. Lieber M., Benveniste R. E., Livingston D. M., Todaro G. J. 1973; Mammalian cells in culture frequently release type-C viruses. Science 183:56–58
    [Google Scholar]
  10. Manly K. F. 1975; A continuous flow system for the growth of RNA tumor viruses. Analytical Biochemistry 63:491–500
    [Google Scholar]
  11. Teich N., Lowy D. R., Hartley J. W., Rowe W. P. 1973; Studies of the mechanism of induction of infectious murine leukemia virus from AKR mouse embryo cell lines by 5-iododeoxyuridine and 5-bromodeoxyuridine. Virology 51:163–173
    [Google Scholar]
  12. Tihon C., Green M. 1973; Cyclic AMP-amplified replication of RNA tumor virus-like particles in Chinese hamster ovary cells. Nature New Biology 244:227–231
    [Google Scholar]
  13. Tihon C., Hellman A. 1976; Characterization of an endogenous Chinese hamster virus. Federation Proceedings 35:1612
    [Google Scholar]
  14. Todaro G. J. 1973; Tumor viruses in normal and transformed cells. In Persistent Virus Infections, Perspectives in Virology VIII pp 81–101 Edited by Pollard M. N.Y.: Academic Press;
    [Google Scholar]
  15. Waqar M. A., Minkoff R., Tsai A., Huberman I. A. 1975; Evidence for RNA linked to nascent DNA in eukaryotic organisms. In DNA Synthesis and Its Regulation Edited by Goulian M. M., Hanawalt P. C., Fox C. F. Menlo Park, California: W. A. Benjamin, Inc;
    [Google Scholar]
  16. Wright B. S., O’Brien P. A., Shibley G. P., Mayyasi S. A., Lasfargues J. C. 1967; Infection of an established mouse bone marrow cell line (JLS-V9) with Rauscher and Moloney murine leukemia viruses. Cancer Research 27:1672–1677
    [Google Scholar]
  17. Zeigel R. F., Clark H. F. 1974; Electron microscopy of the suckling mouse cataract agent: a noncultivable animal pathogen possibly related to mycoplasma. Infection and Immunity 9:430–443
    [Google Scholar]
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