1887

Abstract

SUMMARY

The genomic RNA of human coronavirus strain 229E (HCV 229E) migrated on polyacrylamide gels as a single peak with a mol. wt. of 5.8 × 10. Denaturation of the genome with formaldehyde did not alter its electrophoretic mobility, which suggests that the HCV 229E genome is a single-stranded molecule. At least 30% of the genomic RNA was shown to contain covalently attached polyadenylic acid [poly(A)] sequences by binding the RNA to an oligo(dT)-cellulose column. These poly(A) tracts were shown to be about 70 nucleotides in length by measuring the resistance to digestion of HCV 229E RNA with pancreatic and T RNases. Finally, the genomic RNA was shown to terminate at or near the 3′-terminus on the basis of its susceptibility to polynucleotide phosphorylase.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-39-3-497
1978-06-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/39/3/JV0390030497.html?itemId=/content/journal/jgv/10.1099/0022-1317-39-3-497&mimeType=html&fmt=ahah

References

  1. Aviv H., Leder P. 1972; Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proceedings of the National Academy of Sciences of the United States of America 69:1408–1412
    [Google Scholar]
  2. Garwes D. J., Pocock D. H., Wijaszka T. M. 1975; Identification of heat-dissociable RNA complexes in two porcine coronaviruses. Nature, London 257:508–510
    [Google Scholar]
  3. Kirby K. S. 1965; Isolation and characterization of ribosomal ribonucleic acid. Biochemical Journal 96:266–269
    [Google Scholar]
  4. Loening U. E. 1967; The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochemical Journal 102:251–257
    [Google Scholar]
  5. Lomniczi B. 1977; Biological properties of avian coronavirus RNA. Journal of General Virology 36:531–533
    [Google Scholar]
  6. Macnaughton M. R., Freeman K. B., Bishop J. O. 1974; A precursor to hemoglobin mRNA in nuclei of immature duck red blood cells. Cell 1:117–125
    [Google Scholar]
  7. Macnaughton M. R., Cooper J. A., Dimmock N. J. 1976; Rhinovirus multistranded RNA: dependence of the replicative form on the presence of actinomycin D. Journal of Virology 18:926–932
    [Google Scholar]
  8. Macnaughton M. R., Madge M. H. 1977; The characterisation of the virion RNA of avian infectious bronchitis virus. FEBSLetters 77:311–313
    [Google Scholar]
  9. Mcintosh K. 1974; Coronaviruses: a comparative review. Current Topics in Microbiology and Immunology 63:85–129
    [Google Scholar]
  10. Peterman M. L., Pavlovec A. 1966; The subunits and structural ribonucleic acids of Jensen sarcoma ribosomes. Biochimica et Biophysica Acta 114:264–276
    [Google Scholar]
  11. Schochetman G., Stevens R. H., Simpson R. W. 1977; Presence of infectious polyadenylated RNA in the coronavirus avian bronchitis virus. Virology 77:772–782
    [Google Scholar]
  12. Sheldon R., Kates J., Kelley D. E., Perry R. P. 1972; Polyadenylic acid sequences on 3′-termini of vaccinia messenger ribonucleic acid and mammalian nuclear and messenger ribonucleic acid. Biochemistry 11:3829–3834
    [Google Scholar]
  13. Tannock G. A. 1973; The nucleic acid of infectious bronchitis virus. Archiv fiir die gesamte Virusforschung 43:259–271
    [Google Scholar]
  14. Tannock G. A., Hierholzer J. C. 1977; The RNA of human coronavirus OC-43. Virology 78:500–510
    [Google Scholar]
  15. Tyrrell D. A. J., Almeida J. D., Berry D. M., Cunningham C. H., Hamre D., Hofstad M. S., Mallucci L., Mcintosh K. 1968; Coronaviruses. Nature, London 220:650
    [Google Scholar]
  16. Watkins H., Reeve P., Alexander D. J. 1975; The ribonucleic acid of infectious bronchitis virus. Archives of Virology 47:279–286
    [Google Scholar]
  17. Weinberg R. A., Penman S. 1970; Processing of 45 S nucleolar RNA. Journal of Molecular Biology 47:169–178
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-39-3-497
Loading
/content/journal/jgv/10.1099/0022-1317-39-3-497
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error