1887

Abstract

SUMMARY

Optical diffraction studies of electron micrographs of potato virus T particles have been made to try to understand the variation in appearance of the particles under different staining conditions. These studies show that the particles are constructed from a helical arrangement of protein subunits lying on a primary helix of pitch 3.4 nm. They also suggest that the different surface patterns observed on the virus particles are due to stain lying in secondary helical grooves which are formed by aggregation of the structural protein subunits. The optical diffraction patterns indicate a variation in the periodicity of the secondary helix, which implies a variation in the number of structural protein subunits per turn of the primary helix.

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1978-05-01
2022-01-26
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References

  1. Caspar D. L. D., Klug A. 1962; Physical principles in the construction of regular viruses. Cold Spring Harbor Symposia on Quantitative Biology 27:1–24
    [Google Scholar]
  2. Goodman R. M., McDonald J. G., Horne R. W., Bancroft J. B. 1976; Assembly of flexuous plant Viruses and their proteins. Philosophical Transactions of the Royal Society Series B 276:173–179
    [Google Scholar]
  3. Horne R. W., Ronchetti I. P. 1974; A negative staining-carbon film technique for studying viruses in the electron microscope. I. Preparative procedures for examining icosahedral and filamentous viruses. Journal of Ultrastructure Research 47:361–383
    [Google Scholar]
  4. Horne R. W., Hobart J. M., Ronchetti I. P. 1975; Application of the negative staining-carbon technique to the study of virus particles and their components by electron microscopy. Micron 5:233–261
    [Google Scholar]
  5. Klug A., Berger J. E. 1964; An optical method for the analysis of periodicities in electronmicrographs and some observations on the mechanism of negative staining. Journal of Molecular Biology 10:565–569
    [Google Scholar]
  6. Moody M. F. 1967; Structure of the sheath of bacteriophage T4. 1. Structure of the contracted sheath and polysheath. Journal of Molecular Biology 25:167–200
    [Google Scholar]
  7. O’brien E. J., Bennett P. M. 1972; Structure of straight flagella from a mutant Salmonella. Journal of Molecular Biology 70:133–152
    [Google Scholar]
  8. Robinson D. J., Hutcheson A., Tollin P., Wilson H. R. 1975; A double-helical structure for re-aggregated protein of narcissus mosaic virus. Journal of General Virology 29:325–330
    [Google Scholar]
  9. Salazar L. F., Harrison B. D. 1977; Two previously undescribed viruses from South America. Nature, London 265:337–338
    [Google Scholar]
  10. Taylor C. A., Lipson H. 1964; Optical Transforms: Their Preparation and Application to X-ray Diffraction Problems. London: G. Bell and Sons, Ltd;
    [Google Scholar]
  11. Tollin P., Wilson H. R., Young D. W. 1968; X-ray diffraction evidence of the helical structure of narcissus mosaic virus. Journal of Molecular Biology 34:189–192
    [Google Scholar]
  12. Tollin P., Wilson H. R., Mowat W. P. 1975; Optical diffraction from particles of narcissus mosaic virus. Journal of General Virology 29:331–337
    [Google Scholar]
  13. Varma A., Gibbs A. J., Woods R. D., Finch J. T. 1968; Some observations on the structure of the filamentous particles of several plant viruses. Journal of General Virology 2:107–114
    [Google Scholar]
  14. Wachtel E. J., Marvin F. J., Marvin D. A. 1976; Structural transition in a filamentous protein. Journal of Molecular Biology 107:379–383
    [Google Scholar]
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