RT Journal Article SR Electronic(1) A1 Yoshida, I. A1 Azuma, M. A1 Suenaga, T. A1 Mizuno, F.YR 1978 T1 Regulation of Interferon Production by Dibutyryl Cyclic GMP in Serum-Free Human Diploid Cell Cultures JF Journal of General Virology, VO 39 IS 2 SP 303 OP 310 DO https://doi.org/10.1099/0022-1317-39-2-303 PB Microbiology Society, SN 1465-2099, AB SUMMARY The mechanism for regulating interferon production was investigated in relation to accentuation of production in serum-free human diploid cells (strain WI-38) treated with N 2, O 2-dibutyryl guanosine 3′, 5′-cyclic monophosphate (db-cyclic GMP). Interferon production in serum-free WI-38 cell cultures in response to Newcastle disease virus (NDV) was greatly reduced. In these cells, there was decreased incorporation of 5-3H-uridine into the acid-insoluble fraction, but unimpaired incorporation of U-14C-l-leucine, as compared with serum-containing cultures. When serum-free cell cultures were treated with 0.2 mm-db-cyclic GMP, incorporation of both 5-3H-uridine and U-14C-l-leucine was increased and there was an 8-fold enhancement in the yield of interferon in response to NDV. Induction of db-cyclic GMP-treated cells by NDV in the presence of cycloheximide and actinomycin D suggests that db-cyclic GMP enhances transcription of the interferon gene, and thereby augments interferon production., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-39-2-303