%0 Journal Article %A Yoshida, I. %A Azuma, M. %A Suenaga, T. %A Mizuno, F. %T Regulation of Interferon Production by Dibutyryl Cyclic GMP in Serum-Free Human Diploid Cell Cultures %D 1978 %J Journal of General Virology, %V 39 %N 2 %P 303-310 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-39-2-303 %I Microbiology Society, %X SUMMARY The mechanism for regulating interferon production was investigated in relation to accentuation of production in serum-free human diploid cells (strain WI-38) treated with N 2, O 2-dibutyryl guanosine 3′, 5′-cyclic monophosphate (db-cyclic GMP). Interferon production in serum-free WI-38 cell cultures in response to Newcastle disease virus (NDV) was greatly reduced. In these cells, there was decreased incorporation of 5-3H-uridine into the acid-insoluble fraction, but unimpaired incorporation of U-14C-l-leucine, as compared with serum-containing cultures. When serum-free cell cultures were treated with 0.2 mm-db-cyclic GMP, incorporation of both 5-3H-uridine and U-14C-l-leucine was increased and there was an 8-fold enhancement in the yield of interferon in response to NDV. Induction of db-cyclic GMP-treated cells by NDV in the presence of cycloheximide and actinomycin D suggests that db-cyclic GMP enhances transcription of the interferon gene, and thereby augments interferon production. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-39-2-303