
Full text loading...
The mechanism for regulating interferon production was investigated in relation to accentuation of production in serum-free human diploid cells (strain WI-38) treated with N 2, O 2-dibutyryl guanosine 3′, 5′-cyclic monophosphate (db-cyclic GMP).
Interferon production in serum-free WI-38 cell cultures in response to Newcastle disease virus (NDV) was greatly reduced. In these cells, there was decreased incorporation of 5-3H-uridine into the acid-insoluble fraction, but unimpaired incorporation of U-14C-l-leucine, as compared with serum-containing cultures. When serum-free cell cultures were treated with 0.2 mm-db-cyclic GMP, incorporation of both 5-3H-uridine and U-14C-l-leucine was increased and there was an 8-fold enhancement in the yield of interferon in response to NDV. Induction of db-cyclic GMP-treated cells by NDV in the presence of cycloheximide and actinomycin D suggests that db-cyclic GMP enhances transcription of the interferon gene, and thereby augments interferon production.
Article metrics loading...
Full text loading...
References
Data & Media loading...