1887

Abstract

SUMMARY

The mechanism for regulating interferon production was investigated in relation to accentuation of production in serum-free human diploid cells (strain WI-38) treated with , -dibutyryl guanosine 3′, 5′-cyclic monophosphate (db-cyclic GMP).

Interferon production in serum-free WI-38 cell cultures in response to Newcastle disease virus (NDV) was greatly reduced. In these cells, there was decreased incorporation of 5-H-uridine into the acid-insoluble fraction, but unimpaired incorporation of U-C--leucine, as compared with serum-containing cultures. When serum-free cell cultures were treated with 0.2 m-db-cyclic GMP, incorporation of both 5-H-uridine and U-C--leucine was increased and there was an 8-fold enhancement in the yield of interferon in response to NDV. Induction of db-cyclic GMP-treated cells by NDV in the presence of cycloheximide and actinomycin D suggests that db-cyclic GMP enhances transcription of the interferon gene, and thereby augments interferon production.

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/content/journal/jgv/10.1099/0022-1317-39-2-303
1978-05-01
2024-04-18
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References

  1. Azuma M. 1973; Participation of cytophilic antibody in enhancement of interferon production in macrophages by underneutralized NDV. Archiv für die gesamte Virusforschung 41:11–19
    [Google Scholar]
  2. Azuma M. 1976; Viral factors required for interferon induction by Newcastle disease virus in mouse macrophages and chicken embryo cells. The Journal of General Virology 30:51–62
    [Google Scholar]
  3. Azuma M., Suenaga T., Yoshida I., Mizuno F. 1978; Interferon synthesis in human diploid cells pretreated with insulin. Antimicrobial Agents and Chemotherapy (in the press)
    [Google Scholar]
  4. Billiau A., Joniau M., De Somer P. 1972; Production of crude human interferon with high specific activity. Proceedings of the Society for Experimental Biology and Medicine 140:485–491
    [Google Scholar]
  5. Bdolach A., Schramm M. 1965; The function of 3′, 5′-cyclic AMP in enzyme secretion. Biochemical and Biophysical Research Communications 18:452–456
    [Google Scholar]
  6. Dianzani F., Neri P., Zucca M. 1972; Effect of dibutyryl cyclic AMP on interferon production by cells treated with viral or nonviral inducers. Proceedings of the Society for Experimental Biology and Medicine 140:1375–1378
    [Google Scholar]
  7. Hadhazy G. Y., Strander H., Cantell K. 1969; Serum requirement for interferon production by suspended human leucocytes: studies on action of serum. The Journal of General Virology 5:351–358
    [Google Scholar]
  8. Havell E. A., Vilček J. 1972; Production of high-titered interferon in cultures of human diploid cells. Antimicrobial Agents and Chemotherapy 2:476–484
    [Google Scholar]
  9. Otten J., Johnson G. S., Pastan I. 1972; Regulation of cell growth by cyclic adenosine 3′, 5′-mono-phosphate. The Journal of Biological Chemistry 247:7082–7087
    [Google Scholar]
  10. Reizin F. N., Roikhel V. M., Chumakov M. P. 1975; The influence of substances changing the intracellular concentration of cyclic adenosine 3′, 5′-monophosphate on interferon synthesis in chick embryo cell culture. Archives of Virology 49:307–315
    [Google Scholar]
  11. Rozengurt E., Jlmenez De Asua L. 1973; Role of cyclic 3′, 5′-adenosine monophosphate in the early transport changes induced by serum and insulin in quiescent fibroblasts. Proceedings of the National Academy of Sciences of the United States of America 70:3609–3612
    [Google Scholar]
  12. Rudland P. S., Gospodarowicz D., Seifert W. 1974a; Activation of guanyl cyclase and intracellular cyclic GMP by fibroblast growth factor. Nature, London 250:741–742 773–774
    [Google Scholar]
  13. Rudland P. S., Seeley M., Seifert W. 1974b; Cyclic GMP and cyclic AMP levels in normal and transformed fibroblasts. Nature, London 251:417–419
    [Google Scholar]
  14. Seifert W., Paul D. 1972; Levels of cyclic AMP in sparse and dense cultures of growing and quiescent 3T3 cells. Nature New Biology 240:281–283
    [Google Scholar]
  15. Soeiro R., Amos H. 1966; Arrested protein synthesis in polysomes of cultured chick embryo cells. Science 154:662–665
    [Google Scholar]
  16. Strander H. 1969; Production of interferon in serum-free human leukocyte suspensions. Applied Microbiology 18:810–815
    [Google Scholar]
  17. Weinstein Y., Chambers D. A., Bourne H. R., Melmon K. L. 1974; Cyclic GMP stimulates lymphocyte nucleic acid synthesis. Nature, London 251352–353
    [Google Scholar]
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