1887

Abstract

SUMMARY

The ordering of restriction endonuclease fragments of HSV-2 DNA for physical maps has been studied using molecular hybridization techniques and the cleavage of isolated restriction endonuclease fragments with further restriction endonucleases. Physical maps for the fragments produced by RI, III, II, and I have been constructed. The mol. wt. of the various regions which constitute HSV-2 genome are very similar to the corresponding mol. wt. in the HSV-1 genome.

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/content/journal/jgv/10.1099/0022-1317-39-2-259
1978-05-01
2022-01-21
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References

  1. Clements J. B., Cortini R., Wilkie N. M. 1976; Analysis of herpesvirus DNA structure by means of restriction endonucleases. Journal of General Virology 30:243–256
    [Google Scholar]
  2. Grafstrom R. H., Alwine J. C., Steinhart W. L., Hill C. W. 1974; Terminal repetitions in herpes simplix virus type 1 DNA. Cold Spring Harbor Symposia on Quantitative Biology 39:679–681
    [Google Scholar]
  3. Grafstrom R. W., Alwine J. C., Steinhart W. L., Hill C. W., Hyman R. W. 1975; The terminal repetition of herpes simplex virus DNA. Virology 67:144–157
    [Google Scholar]
  4. Hayward G. S., Frenkel N., Roizman B. 1975a; Anatomy of herpes simplex virus DNA: strain differences and heterogeneity in the locations of restriction endonuclease cleavage sites. Proceedings of the National Academy of Sciences of the United States of America 72:1768–1772
    [Google Scholar]
  5. Hayward G. S., Jacob R. J., Wadsworth S. C., Roizman B. 1975b; Anatomy of herpes simplex virus DNA: evidence for four populations of molecules that differ in the relative orientation of their long and short components. Proceedings of the National Academy of Sciences of the United States of America 72:4243–4247
    [Google Scholar]
  6. Kieff D., Bachenheimer S. L., Roizman B. 1971; Size, composition and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2. Journal of Virology 8:125–132
    [Google Scholar]
  7. Share P. A., Sugden B., Sambrook J. 1973; Detection of two restriction endonuclease activities in Hemophilus parainfluenzae using analytical agarose-ethidium bromide electrophoresis. Biochemistry 12:3055–3063
    [Google Scholar]
  8. Sheldrick P., Berthelot N. 1974; Inverted repetitions in the chromosome of herpes simplex virus. Cold Spring Harbor Symposia on Quantitative Biology 39:667–678
    [Google Scholar]
  9. Skare J., Summers W. C. 1977; Structure and function of herpesvirus genomes. II. Eco RI, Xba I and Hind III endonuclease cleavage sites on herpes simplex virus type 1 DNA. Virology 76:581–595
    [Google Scholar]
  10. Skare J., Summers W. P., Summers W. C. 1975; Structure and function of herpesvirus genomes 1. Comparison of five HSV-1 and two HSV-2 strains by cleavage of their DNA with Eco RI restriction endonuclease. Journal of Virology 15:726–732
    [Google Scholar]
  11. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–518
    [Google Scholar]
  12. Wadsworth S., Jacob R. J., Roizman B. 1975; Anatomy of herpes simplex virus DNA II. Size, composition and arrangement of inverted terminal repetitions. Journal of Virology 15:1487–1497
    [Google Scholar]
  13. Wagner E. K., Tewari K. K., Kolodner R., Warner R. C. 1974; The molecular size of herpes simplex virus type 1 genome. Virology 57:436–447
    [Google Scholar]
  14. Wilkie N. M. 1973; The synthesis of herpesvirus DNA: the distribution of alkali-labile single strand interruptions in HSV-1DNA. Journal of General Virology 21:453–467
    [Google Scholar]
  15. Wilkie N. M. 1976; Physical maps for HSV-i DNA for restriction endonucleases Hind III, Hpa I and Xba. Journal of Virology 20:222–233
    [Google Scholar]
  16. Wilkie N. M., Clements J. B., Macnab J. C. M., Subak-Sharpe J. H. 1974; The structure and biological properties of herpes simplex virus DNA. Cold Spring Harbor Symposia on Quantitative Biology 39:657–666
    [Google Scholar]
  17. Wilkie N. M., Cortini R. 1976; Sequence arrangement of HSV-1 DNA: identification of terminal fragments in restriction endonuclease digests and evidence for inversions in redundant and unique sequences. Journal of Virology 20:211–221
    [Google Scholar]
  18. Wilkie N. M., Cortini R., Clements J. B. 1977; Structural studies and physical maps for the herpes simplex virus genome. Journal of Antimicrobial Chemotherapy 3:47–62
    [Google Scholar]
  19. Wilkie N. M., Stow N. D., Marsden H. S., Preston V., Cortini R., Timbury M. C., Subak-Sharpe J. H. 1977b; Physical mapping of herpes simplex viius coded functions and polypeptides by marker rescue and analysis of HSV-1 /HSV-2 intertypic recombinants. Oncogenesis and Herpesviruses 111: (in the press)
    [Google Scholar]
  20. Yoshimori R. N. 1971 Ph.D. thesis University of California; San Francisco Medical Center:
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