1887

Abstract

SUMMARY

Adult mice inoculated with Semliki Forest virus (SFV) were protected from a lethal infection of the central nervous system by intranasal administration of defective-interfering (DI) SFV. DI SFV was prepared by eight passages at high m.o.i. in BHK 21 cells. Mice were treated with unpurified, unconcentrated tissue culture fluid which had been u.v.-irradiated to inactivate the infective virus present. Prevention of death was maximal when the DI virus was administered simultaneously with the infecting inoculum, and under the same conditions multiplication of infective virus in the brains of treated mice was reduced by 10-fold. It was shown that DI SFV was propagated in mouse brains following intranasal inoculation and it was concluded that protection was brought about through the intrinsic interfering capacity of the DI virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-39-2-231
1978-05-01
2022-08-10
Loading full text...

Full text loading...

/deliver/fulltext/jgv/39/2/JV0390020231.html?itemId=/content/journal/jgv/10.1099/0022-1317-39-2-231&mimeType=html&fmt=ahah

References

  1. Atkins O. J., Johnston M. D., Westmacott L. M., Burke D. C. 1974a; Induction of interferon in chick cells by temperature-sensitive mutants of Sindbis virus. Journal of General Virology 25:381–390
    [Google Scholar]
  2. Atkins O. J., Samuels J., Kennedy S. I. T. 1974b; Isolation and preliminary characterization of temperature-sensitive mutants of Sindbis virus strain AR339. Journal of General Virology 25:371–380
    [Google Scholar]
  3. Bradish C. J., Allner K., Fitzoeorge R. 1975; Immunomodification and the expression of virulence in mice by defined strains of Semliki Forest virus: the effects of cyclophosphamide. Journal of General Virology 28:225–237
    [Google Scholar]
  4. Bruton C. J., Kennedy S. I. T. 1975; Semliki Forest virus intracellular RNA: properties of the multistranded RNA species and kinetics of positive and negative strand synthesis. Journal of General Virology 28:111–127
    [Google Scholar]
  5. Bruton C. J., Kennedy S. I. T. 1976; Defective-interfering particles of Semliki Forest virus: structural differences between standard virus and defective-interfering particles. Journal of General Virology 31:383–395
    [Google Scholar]
  6. Bruton C. J., Porter A., Kennedy S. I. T. 1976; Defective-interfering particles of Semliki Forest virus: intracellular events during interference. Journal of General Virology 31:397–416
    [Google Scholar]
  7. Clegg J. C. S., Kennedy S. I. T. 1974; Polyadenylic acid sequences in the virus RNA species of cells infected with Semliki Forest virus. Journal of General Virology 22:331–345
    [Google Scholar]
  8. Dalrymple J. M., Teravoto A. Y., Cardiff R. D., Russell P. K. 1972; Radioimmune precipitation of group A arboviruses. Journal of Immunology 109:426–433
    [Google Scholar]
  9. Doyle M., Holland J. J. 1973; Prophylaxis and immunisation in mice by use of virus-free defective T particles to protect against intracerebral infection by vesicular stomatitis virus. Proceedings of the National Academy of Sciences of the United States of America 70:2105–2108
    [Google Scholar]
  10. Henderson D. W., Peacock S., Randles W. J. 1967; On the pathogenesis of Semliki Forest virus (SFV) infection in the hamster. British Journal of Experimental Pathology 48:228–234
    [Google Scholar]
  11. Holland J. J., Doyle M. 1973; Attempts to detect homologous autointerference in vivo with influenza virus and vesicular stomatitis virus. Infection and Immunity 7:526–531
    [Google Scholar]
  12. Holland J. J., Villarreal L. P. 1975; Purification of defective interfering T particles of vesicular stomatitis and rabies viruses generated in vivo in brains of newborn mice. Virology 67:438–449
    [Google Scholar]
  13. Huang A. S. 1973; Defective interfering viruses. Annual Review of Microbiology 27:101–117
    [Google Scholar]
  14. Huang A. S., Baltimore D. 1970; Defective viral particles and viral disease processes. Nature, London 226:325–327
    [Google Scholar]
  15. Johnson R. T., Mims C. A. C. 1968; Pathogenesis of viral infections of the nervous system. New England Journal of Medicine 278:23–30
    [Google Scholar]
  16. Kennedy S. I. T. 1974; The effect of enzymes on structural and biological properties of Semliki Forest virus. Journal of General Virology 23:129–143
    [Google Scholar]
  17. Kennedy S. I. T., Burke D. C. 1972; Studies on the structural proteins of Semliki Forest virus. Journal of General Virology 14:87–98
    [Google Scholar]
  18. Lehmann-Grube F. 1971; Lymphocytic choriomeningitis virus. Virology Monographs 10:1–173
    [Google Scholar]
  19. Mims C. A. C. 1956; Rift Valley fever in mice. IV. Incomplete virus: its production and properties. British Journal of Experimental Pathology 37:129–143
    [Google Scholar]
  20. Morser M. J., Kennedy S. I. T., Burke D. C. 1973; Virus-specified polypeptides in cells infected with Semliki Forest virus. Journal of General Virology 21:19–29
    [Google Scholar]
  21. Nir Y., Beemer A., Goldwasser R. A. 1965; West Nile virus infection in mice following exposure to a viral aerosol. British Journal of Experimental Pathology 46:443–449
    [Google Scholar]
  22. Rabin E. R., Jenson A. B., Melnick J. L. 1968; Herpes Simplex in mice: electron microscopy of neural spread. Science 162:126–127
    [Google Scholar]
  23. Von magnus P. 1951; Propagation of the PR 8 strain of influenza virus in chick embryos. III. Properties of the incomplete virus produced in serial passages of undiluted virus. Acta Pathohgica et Micro-biologica Scandinavica 29:156–18
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-39-2-231
Loading
/content/journal/jgv/10.1099/0022-1317-39-2-231
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error