1887

Abstract

SUMMARY

The morphogenesis of porcine rotavirus was similar in porcine kidney (PK) cell cultures and in porcine epithelial cells as examined by electron microscopy. Infected cells contained cytoplasmic, non-membrane-bound viroplasm and accumulations of virus particles within cisternae of the rough endoplasmic reticulum (RER). Three types of virus particles were noted: double-shelled or complete particles which averaged 77 nm in diam.; single-shelled or naked particles which ranged from 50 to 55 nm in diam.; and electron-dense nucleoids, or cores, 31 to 38 nm in diam. Virus particles acquired outer shells by budding through either matrices of granular, electron-dense viroplasm or membranes of distended RER. Accumulation of numerous single-shelled particles was observed only in PK cell cultures containing a high percentage of infected cells. In these cells, virus release occurred through disruption of the plasma membrane. Tubules, similar in diameter to the single-shelled particles, were observed in the nuclei of a few infected PK cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-39-2-205
1978-05-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/39/2/JV0390020205.html?itemId=/content/journal/jgv/10.1099/0022-1317-39-2-205&mimeType=html&fmt=ahah

References

  1. Adams W. R., Kraft L. 1967; Electron-microscopic study of the intestinal epithelium of mice infected with the agent of epizootic diarrhoea of infant mice (EDIM virus). American Journal of Pathology 51:39–60
    [Google Scholar]
  2. Banfield W. G., Kasnic G., Blackwell J. H. 1968; Further observations on the virus of epizootic diarrhoea of infant mice: an electron microscopic study. Virology 36:411–421
    [Google Scholar]
  3. Bishop R. F., Davidson G. P., Holmes I. H., Ruck B. J. 1973; Virus particles in epithelial cells of duodenal mucosa from children with acute non-bacterial gastroenteritis. Lancet 11:1281–1283
    [Google Scholar]
  4. Bohl E. H., Kohler E. M., Saif L. J., Cross R. F., Agnes A. G., Theil K. W. 1977; Rotavirus as a Cause of diarrhea in pigs. Journal of the American Veterinary Medical Association (in the press)
    [Google Scholar]
  5. Borgers M., Debrabander M., Van Reempts J., Awouters F., Jacobs W. A. 1977; Intranuclear microtubules in lung mast cells of guinea pigs in anaphylactic shock. Laboratory Investigation 37:1–8
    [Google Scholar]
  6. Breese S. S., Ozawa Y., Dardiri A. H. 1969; Electron microscopic characterisation of African horse sickness virus. Journal of the American Veterinary Medical Association 155:391–400
    [Google Scholar]
  7. Chasey D., Lucas M. 1977; Detection of rotavirus in experimentally infected piglets. Research in Veterinary Science 22:124–125
    [Google Scholar]
  8. Dales S., Gomatos P. J., Hsu K. C. 1965; The uptake and development of reovirus in strain L cells followed with labelled viral ribonucleic acid and ferritin antibody complexes. Virology 35:193–211
    [Google Scholar]
  9. Davidson G. P., Bishop R. F., Townley R. R. W., Holmes I. H., Ruck B. J. 1975; Importance of a new virus in acute sporadic enteritis in children. Lancet 1:242–246
    [Google Scholar]
  10. Els H. J., Lecatsas G. 1972; Morphological studies on simian virus S.A. 11 and the ‘related’ O agent. Journal of General Virology 17:129–132
    [Google Scholar]
  11. Flewett T. H., Bryden A. S., Davies H. A. 1973; Virus particles in gastroenteritis. Lancet ii:1497
    [Google Scholar]
  12. Flewett T. H., Bryden A. S., Davies H., Woode G. N., Bridger J. C., Derrick J. M. 1974; Relation between viruses from acute gastroenteritis of children and newborn calves. Lancet ii:61–63
    [Google Scholar]
  13. Frederick G. T., Bohl E. H., Cross R. F. 1976; Pathogenicity of an attenuated strain of transmissible gastroenteritis virus for newborn pigs. American Journal of Veterinary Research 37:165–169
    [Google Scholar]
  14. Harford C. G., Hamlin A., Middelkamp J. N., Briggs D. G. 1962; Electron microscopic examination of cells infected with reovirus. Journal of Laboratory and Clinical Medicine 60:179–193
    [Google Scholar]
  15. Holmes L. H., Ruck B. J., Bishop R. F., Davidson G. P. 1975; Infantile enteritis viruses: morphogenesis and morphology. Journal of Virology 16:937–943
    [Google Scholar]
  16. Kapikian A. Z., Cline W. L., Mebus C. A., Wyatt R. G., Kalica A. R., James H. D., Vankirk D., Chanock R. M., Kim H. W. 1975; New complement-fixation test for the human reovirus-like agent of infantile gastroenteritis. Lancet i:1056–1061
    [Google Scholar]
  17. Kapikian A. Z., Kim H. W., Wyatt R. G., Rodriguez W. J., Ross S., Cline W. L., Parrot R. H., Chanock R. M. 1974; Reoviruslike agent in stools: association with infantile diarrhea and development of serologic tests. Science 185:1049–1053
    [Google Scholar]
  18. Lecatsas G. 1968a; Electron microscopic study of the formation of bluetongue virus. Onderstepoort Journal of Veterinary Research 35:139–150
    [Google Scholar]
  19. Lecatsas G. 1968b; Electron microscopic studies on reovirus type 1 in BE1K2I cells. Onderstepoort Journal of Veterinary Research 35:151–158
    [Google Scholar]
  20. Lecatsas G. 1972; Electron microscopic and serological studies on simian virus S.A.n and the ‘related’ O agent. Onderstepoort Journal of Veterinary Research 39:133–138
    [Google Scholar]
  21. Mcnulty M. S., Allan G. M., Pearson G. R., Mcferran J. B., Curran W. L., Mccracken R. M. 1976a; Reovirus-like agent (rotavirus) from lambs. Infection and Immunity 14:1332–1338
    [Google Scholar]
  22. Mcnulty M. S., Curran W. L., Mcferran J. B. 1976b; The morphogenesis of a cytopathic bovine rotavirus in Madin-Darby bovine kidney cells. Journal of General Virology 33:503–508
    [Google Scholar]
  23. Mcnulty M. S., Pearson G. R., Mcferran J. B., Collins D. S., Allan G. M. 1976c; A reOviruS-like agent (rotavirus) associated with diarrhea in neonatal pigs. Veterinary Microbiology r:55–63
    [Google Scholar]
  24. Mebus C. A., Stair E. L., Underdahl N. R., Twiehaus M. J. 1971; Pathology of neonatal calf diarrhea induced by a reo-like virus. Veterinary Pathology 8:490–505
    [Google Scholar]
  25. Mebus C. A., Underdahl N. R., Rhodes M. B., Twiehaus M. J. 1969; Calf diarrhea (scours) reproduced with a virus from a field outbreak. University of Nebraska Research Bulletin 233:1–16
    [Google Scholar]
  26. Much D. H., Zajac I. 1972; Purification and characterization of epizootic diarrhea of infant mice virus. Infection and Immunity 6:1019–1024
    [Google Scholar]
  27. Murphy F. A., Borden E. C., Shope R. E., Harrison A. 1971; Physicochemical and morphological relationships of some arthropod-borne viruses to bluetongue virus - a new taxonomic group. Electron microscopic studies. Journal of General Virology 13:273–288
    [Google Scholar]
  28. Murphy F. A., Coleman P. H., Harrison A. K., Gary G. W. 1968; Colorado tick fever virus; an electron microscopic study. Virology 35:28–40
    [Google Scholar]
  29. Rhim J. S., Jorden L. E., Mayor H. D. 1962; Cytochemical, fluorescent-antibody and electron microscopic studies on the growth of reovirus (ECHO 10) in tissue culture. Virology 17:342–355
    [Google Scholar]
  30. Rubinstein D., Milne R. G., Buckland R., Tyrrell D. A. J. 1971; The growth of the virus of epidemic diarrhoea of infant mice (EDIM) in organ cultures of intestinal epithelium. British Journal of Experimental Pathology 52:442–445
    [Google Scholar]
  31. Saif L. J., Bohl E. H., Kohler E. M., Hughes J. H. 1977; Immune electron microscopy of transmissible gastroenteritis virus and rotavirus (reovirus-like agent) of swine. American Journal of Veterinary Research 38:13–20
    [Google Scholar]
  32. Snodgrass D. R., Smith W., Gray E. W., Herring J. A. 1976; A rotavirus in lambs with diarrhea. Research in Veterinary Science 20:113–114
    [Google Scholar]
  33. Stair E. L., Mebus C. A., Twiehaus M. J., Underdahl N. R. 1973; Neonatal calf diarrhoea; electron microscopy of intestines infected with a reovirus-like agent. Veterinary Pathology 10:155–170
    [Google Scholar]
  34. Theil K. W., Bohl E. H., Agnes A. G. 1977; Cell culture propagation of porcine rotavirus (reovirus-like agent). American Journal of Veterinary Research 38:1765–1768
    [Google Scholar]
  35. Theil K. W., Bohl E. H., Cross R. F., Kohler E. M., Agnes A. G. 1978; Pathogenesis of porcine rotaviral infection in experimentally inoculated gnotobiotic pigs. American Journal of Veterinary Research (in the press)
    [Google Scholar]
  36. Tsai K., Karstad L. 1970; Epizootic hemorrhagic disease virus of deer; an electron microscopic study. Canadian Journal of Microbiology 16:427–432
    [Google Scholar]
  37. Woode G. N., Bridger J. C., Hall G., Dennis M. J. 1974; The isolation of a reovirus-like agent associated with diarrhea in colostrum-deprived calves in Great Britain. Research in Veterinary Science 16:102–105
    [Google Scholar]
  38. Woode G. N., Bridger J., Hall G. A., Jones J. M., Jackson G. 1976; The isolation of reovirus-like agents (rotaviruses) from acute gastroenteritis of piglets. Journal of Medical Microbiology 9:203–209
    [Google Scholar]
  39. Wyatt R. G., Gill V. W., Sereno M. M., Kalica A. R., Van kirk D. H., Chanock R. M., Kapikian A. Z. 1976; Probable in vitro cultivation of human reovirus-like agent of infantile diarrhea. Lancet i:98–99
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-39-2-205
Loading
/content/journal/jgv/10.1099/0022-1317-39-2-205
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error