1887

Abstract

SUMMARY

Human coronavirus RNA, prepared by extraction of purified virions with phenol-chloroform, consists of a major 15 to 55S class and a minor 4S class of RNA fragments. Polyadenylic acid [poly (A)] sequences are present in 15 to 55S but not in 4S RNA, suggesting different functions for each class. A stretch of poly (A) of approximately 19 adenosine monophosphate residues was obtained in sizing experiments after digesting OC-43 RNA with pancreatic and T ribonucleases. An OC-43 virion RNA transcriptase could not be detected with systems optimal for detecting the transcriptases of influenza and Newcastle disease virus.

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1978-04-01
2022-01-28
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References

  1. Akers T. G. 1963; Cited by C. H. Cunningham in ‘Newer knowledge of infectious bronchitis virus’. Proceedings of the XVIIth World Veterinary Congress 1:607–610
    [Google Scholar]
  2. Baltimore D. 1971; Expression of animal virus genomes. Bacteriological Reviews 35:235–241
    [Google Scholar]
  3. Bingham R. W. 1975; Some biological characteristics of a coronvirus-avian infectious bronchitis virus. Abstracts of the yd International Congress for Virology Madrid, P 73:
    [Google Scholar]
  4. Dahlberg J. E., Harada F., Sawyer R. C. 1974; Structure and properties of an RNA primer for initiation of Rous sarcoma virus DNA synthesis in vitro. Cold Spring Harbor Symposium on Quantitative Biology 39:925–932
    [Google Scholar]
  5. Erikson E., Erikson R. L. 1971; Association of 4S ribonucleic acid with oncornavirus ribonucleic acids. Journal of Virology 8:254–256
    [Google Scholar]
  6. Garwes D. J., Pocock D. H., Wijaszka T. M. 1975; Identification of heat-dissociable RNA complexes in two porcine coronaviruses. Nature, London 257:508–510
    [Google Scholar]
  7. Hierholzer J. C. 1976; Purification and biophysical properties of human coronavirus 229E. Virology 75:155–165
    [Google Scholar]
  8. Huang A. S., Baltimore D., Bratt M. A. 1971; Ribonucleic acid polymerase in virions of Newcastle disease virus: comparison with the vesicular stomatitis virus polymerase. Journal of Virology 7:389–394
    [Google Scholar]
  9. Johnston R. E., Bose H. R. 1972; Correlation of messenger function with adenylate-rich segments in the genomes of single-stranded RNA viruses. Proceedings of the National Academy of Sciences of the United States of America 69:1514–1516
    [Google Scholar]
  10. Kennedy D. A., Johnson-Lussenburg C. M. 1976; Isolation and morphology of the internal component of human coronavirus, strain 229E. Intervirology 6:197–206
    [Google Scholar]
  11. King A. M. Q., Wells R. D. 1976; All intact subunit RNAs from Rous sarcoma virus contain poly (A). Journal of Biological Chemistry 251:150–152
    [Google Scholar]
  12. Lai M. M. C., Duesberg P. H. 1972; Adenylic acid-rich sequence in RNAs of Rous sarcoma virus and Rauscher mouse leukaemia virus. Nature, London 235:383–386
    [Google Scholar]
  13. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  14. Mcintosh K., Becker W. B., Chanock R. M. 1967; Growth in suckling-mouse brain of ‘IBV-like’ viruses from patients with upper respiratory tract disease. Proceedings of the National Academy of Sciences of the United States of America 58:2268–2273
    [Google Scholar]
  15. Newman J. F. E., Brown F. 1976; Absence of poly (A) from infective RNA of Nodamura virus. Journal of General Virology 30:137–140
    [Google Scholar]
  16. Oxford J. S., Perrin D. D. 1974; Inhibition of the particle-associated RNA-dependent RNA polymerase activity of influenza viruses by chelating agents. Journal of General Virology 23:59–71
    [Google Scholar]
  17. Schochetman G., Stevens R. H., Simpson R. W. 1977; Presence of infections polyadenylated RNA in the coronavirus avian bronchitis virus. Virology 77:772–782
    [Google Scholar]
  18. Spirin A. S. 1962; Some problems concerning the macromolecular structure of ribonucleic acids. Progess in Nucleic Acid Research and Molecular Biology 1:301–345
    [Google Scholar]
  19. Stephenson M. L., Scott J. F., Zamecnik P. C. 1973; Evidence that the polyadenylic acid of ‘35s’ RNA of avian myeloblastitis virus is located at the 3′-OH terminus. Biochemical and Biophysical Research Communications 55:8–16
    [Google Scholar]
  20. Tannock G. A. 1973; The nucleic acid of infectious bronchitis virus. Archiv für die gesamte Virusforschung 43:259–271
    [Google Scholar]
  21. Tannock G. A., Gibbs A. J., Cooper P. D. 1970; A re-examination of the molecular weight of poliovirus RNA. Biochemical and Biophysical Research Communications 38:298–304
    [Google Scholar]
  22. Tannock G. A., Hierholzer J. C. 1977; The RNA of human coronavirus OC-43. Virology 78:500–510
    [Google Scholar]
  23. Temin H. M., Baltimore D. 1972; RNA-directed synthesis and RNA tumour viruses. Advances in Virus Research 17:129–186
    [Google Scholar]
  24. Wang L. H., Duesberg P. 1974; Properties and location of poly (A) in Rous sarcoma virus RNA. Journal of Virology 14:1515–1529
    [Google Scholar]
  25. Watkins H., Reeve P., Alexander D. J. 1975; The ribonucleic acid of infectious bronchitis virus. Archives of Virology 47:279–286
    [Google Scholar]
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