1887

Journal of General Virology header logo

Volume 38, Issue 3

Deposition of Retrovirus Associated Antigens (p30 and gp70) on Cell Membranes of Feline and Murine Leukaemia Virus Infected Cells Free

Abstract

SUMMARY

A quantitative estimation of retrovirus associated cell membrane antigens of murine and feline cells infected with their respective type C leukosis virus is presented. Using a radio-immune assay with three broadly reactive antisera, the minimum estimated number of retrovirus associated antigenic determinants on YAC [Moloney leukaemia virus (MuLV) infected murine] and FL-74 [feline leukaemia virus (FeLV) infected feline] cells was 1.3 × 10 and 1.6 × 10 determinants per cell respectively. The virus structural proteins p27–30 and gp70 were detected by three component specific antisera on murine and feline cell surfaces in amounts which varied between cell isolates. MuLV infected cells produced as many as 1.9 × 10 p30 antigenic determinants and 7.5 × 10 gp70 determinants on infected cells. FeLV infected cells (FL-74) expressed 5.6 × 10 p27 and 7.5 × 10 gp70 antigenic determinants per single cell surface. The major core protein (p27–30) and the major envelope glycoprotein (gp70) antigens are sufficiently physically separated on cell surfaces so that binding of either of the membrane antigens with component specific antibodies does not interfere with binding of antibodies specific for the other. Despite the expression of interspecies determinants for p30, gp70, and other retrovirus associated antigens detected by antibody procedures, interspecies determinants of cell mediated immunity could not be demonstrated in immune mice bearing Moloney sarcoma virus (MSV) induced tumours. Furthermore, xenogeneic immunization of mice with FL-74 cells failed to protect mice against the growth of MSV induced lymphoma or sarcoma.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1978
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-38-3-483
1978-03-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/38/3/JV0380030483.html?itemId=/content/journal/jgv/10.1099/0022-1317-38-3-483&mimeType=html&fmt=ahah

References

  1. Boone C. W., Gordin F., Kawakami T. G. 1973; Surface antigens on cat leukemia cells induced by feline leukemia virus: area diversity and antibody binding affinity. Journal of Virology 11:515–519
     [Google Scholar]
  2. Boone C. W., Irving I. N., Rubinstein S. 1971; Quantitative studies on the binding of antibody to the surface of HeLa cells. Journal of Immunology 106:879–887
     [Google Scholar]
  3. Boyse E. A., Old L. J., Stockert E. 1968; An approach to the mapping of antigens on the cell surface. Proceedings of the National Academy of Sciences of the United States of America 60:886–893
     [Google Scholar]
  4. Crandell R. A., Fabricant C. G., Nelson-Rees W. A. 1973; Development, characterization and viral susceptibility of a feline (Felis catus) renal cell line (CRFK). In Vitro 9:176–185
     [Google Scholar]
  5. Davis S., Boone C. W. 1974; The in vivo effects of Concanavalin-A on the cellular immune response in BALB/c mice. Inhibition of the delayed hypersensitivity reaction to tuberculin. European Journal of Immunology 4:626–629
     [Google Scholar]
  6. Davis S., Boone C. W. 1975; Inhibition of the cellular immune response to Simian virus 40 tumor cells in tumor bearing and tumor immune mice by Concanavalin-A. Journal of the National Cancer Institute 54:435–438
     [Google Scholar]
  7. Delarco J., Todaro G. 1976; Membrane receptors for murine leukemia viruses: characterization using the purified viral genome envelope glycoprotein, gp71. Cell 8:365–371
     [Google Scholar]
  8. Essex M. C. 1975; Horizontally and vertically transmitted oncornavirus of cats. Advances in Cancer Research 21:175–248
     [Google Scholar]
  9. Fenyo E. M., Grundner G., Wiener F., Klein E., Klein G., Harris H. 1973; The influence of the partner cell on the production of L virus and the expression of virus surface antigen in hybrid cells. Journal of Experimental Medicine 137:1240–1255
     [Google Scholar]
  10. Fenyo E. M., Klein G. 1976; Independence of Moloney virus induced cell surface antigen (MCSA) and membrane associated virion antigen in immunoselected lymphoma sublines. Nature, London 260:355–357
     [Google Scholar]
  11. Fenyo E. M., Klein E., Klein G., Swich K. 1968; Selection of an immunoresistant Moloney lymphoma subline with decreased concentration of tumor specific surface antigens. Journal of the National Cancer Institute 40:69–89
     [Google Scholar]
  12. Fenyo E. M., Nazerian K., Klein E. 1974; Characteristics of murine C-type viruses. II. The behavior of viruses resident in various cell lines and their hybrids on BALB/3T3 and mouse embryonic fibroblast cultures. Virology 59:574–579
     [Google Scholar]
  13. Fenyo E. M., Nordenskjold B. A., Klein E. 1971; Membrane immunofluorescence on cultured indicator cells as a measure of virus production by mouse L cells and by two Moloney lymphoma sublines differing in immunosensitivity. Annals of the New York Academy of Sciences 177:121–130
     [Google Scholar]
  14. Fleissner E., Ikeda H., Tung J. S., Vitteta E. S., Tress E., Hardy W., Stockert E., Boyse E. A., Pincus T., O’Donnell P. 1974; Characterization of murine leukemia virus specific proteins. Cold SpringHarbor Symposia on Quantitative Biology 34:1057–1066
     [Google Scholar]
  15. Glynn J. B., Halpern B. L., Fefer A. 1969; An immunochemotheropeutic system for the treatment of a transplanted Moloney virus-induced lymphoma in mice. Cancer Research 29:515–520
     [Google Scholar]
  16. Grant J. P., Bigner D. D., Fischinger P. J., Bolognesi D. P. 1974; Expression of murine leukemia virus structural antigens on the surface of chemically induced murine sarcomas. Proceedings of the National Academy of Sciences of the United States of America 71:5037–5041
     [Google Scholar]
  17. Kurth R., Bauer H. 1973; Avian oncornavirus-induced tumor antigens of embryonic and unknown origin. Virology 56:496–504
     [Google Scholar]
  18. Klebe R. J., Chen T., Ruddle F. H. 1970; Controlled production of proliferating somatic cell hybrids. Journal of Cell Biology 45:151–156
     [Google Scholar]
  19. Ledbetter J., Nowinski R., Emery S. 1977; Viral proteins expressed on the surface of murine leukemia cells. Journal of Virology 22:65–73
     [Google Scholar]
  20. Lerner R. A., Wilson C. B., Del Villano B. C., Mcconahey P. J., Dixon F. J. 1976; Endogenous oncornaviral gene expression in adult and fetal mice: quantitative, histologic and physiologic studies of the major viral glycoprotein, gp70. Journal of Experimental Medicine 143:151–166
     [Google Scholar]
  21. Lilly F., Steeves R. 1974; Antigens of murine leukemia viruses. Biochimica et Biophysica Acta 334:105–118
     [Google Scholar]
  22. Littlefield J. W. 1964; Selection of hybrids from matings of fibroblasts in vitro and their presumed recombinants. Science 145:709–710
     [Google Scholar]
  23. Massicot J. G., Woods W. A., Chirigos M. A. 1971; Cell line derived from murine sarcoma virus (Moloney pseudo type(-induced tumor: cultural, antigenic, and virological properties. Applied Microbiology 22:1119–1122
     [Google Scholar]
  24. Obata Y., Ikeda H., Stockert E., Boyse E. A. 1975; Relation of GIX antigen of thymocytes envelope glycoprotein of murine leukemia virus. Journal of Experimental Medicine 141:188–197
     [Google Scholar]
  25. O’Brien S. J., Boone C. W. 1977; Expression of feline leukaemia virus antigens on cat lymphoma cells: kinetics of biosynthesis. Journal of General Virology 35:511–523
     [Google Scholar]
  26. O’Brien S. J., Simonson J. M., Boone C. W. 1976a; Expression of virus structural proteins on murine cell surfaces in association with the production of murine leukaemia virus. Journal of General Virology 33:355–360
     [Google Scholar]
  27. O’Brien S. J., Boone C. W., Simonson J. M., Austin F. 1976b; The paired label assay for cell surface antigens. Tissue Culture Association Manual 2:423–427
     [Google Scholar]
  28. Oroszlan S., Fisher C. L., Stanley T. B., Gilden R. V. 1970; Proteins of the murine C-type RNA tumour viruses: isolation of a group-specific antigen by isoelectric focusing. Journal of General Virology 8:1–10
     [Google Scholar]
  29. Oroszlan S., Huebner R. J., Gilden R. V. 1971; Species specific and interspecific antigen determinants associated with the structural protein of feline C-type virus. Proceedings of the National Academy of Sciences of the United States of America 68:901–904
     [Google Scholar]
  30. Oskarsson M. K., Robey W. G., Harris C. L., Fischinger P. J., Haapala D. K., Vande Woude G. F. 1975; A p60 polypeptide in the feline leukemia virus pseudotype of Moloney sarcoma virus with murine leukemia. Proceedings of the National Academy of Sciences of the United States of America 72:2380–2384
     [Google Scholar]
  31. Paranipe M., Boone C. W. 1972; Delayed hypersensitivity to Simian virus 40 tumor cells in BALB/c mice demonstrated by a radioisotopic foot-pad assay. Journal of the National Cancer Institute 48:563–566
     [Google Scholar]
  32. Parks W. P., Noon M. C., Gilden R., ScoLNicK E. M. 1975; Serological studies with low molecular weight peptides from the Moloney strain of murine leukemia virus. Journal of Virology 15:1385–1395
     [Google Scholar]
  33. Sarma P., Log T. 1973; Subgroup classification of feline leukemia and sarcoma viruses of feline-sarcoma complex. Virology 64:438–446
     [Google Scholar]
  34. Sarma P., Log T., Damini J., Hill P. R., Huebner R. J. 1975; Differential host range of viruses of feline leukaemia-sarcoma complex. Virology 64:438–446
     [Google Scholar]
  35. Scolnick E. M., Parks W. P., Todaro G. J., Aaronson S. A. 1972; Immunological characterization of primate C-type virus reverse transcriptase. Nature New Biology 235:35–40
     [Google Scholar]
  36. Sliski A. H., Essex M., Meyer C., Todaro G. 1977; Feline oncornavirus-associated cell membrane antigen: expression in transformed non producer mink cell. Science 196:1336–1339
     [Google Scholar]
  37. Steeves R. A., Strand M., August J. T. 1974; Structural proteins of mammalian oncogenic RNA viruses: murine leukemia virus neutralization by antisera prepared against purified envelope glycoprotein. Journal of Virology 14:187–189
     [Google Scholar]
  38. Stephenson J. R., Tronick S. R., Aaronson S. A. 1974; Analysis of type specific antigenic determinants of two structural polypeptides of mouse RNA C-type viruses. Virology 58:1–8
     [Google Scholar]
  39. Strand M., August J. T. 1973; Structural properties of oncogenic ribonucleic acid viruses. Interspecies II, a new interspecies antigen. Journal of Biological Chemistry 248:5627–5633
     [Google Scholar]
  40. Strand M., August J. T. 1974; Structural proteins of mammalian oncogenic RNA viruses. Multiple antigenic determinants of the major internal protein and envelope glycoprotein. Journal of Virology 13:171–180
     [Google Scholar]
  41. Strand M., August J. T. 1975; Structural proteins of mammalian RNA tumor viruses: relatedness of interspecies antigenic determinants of the major internal protein. Journal of Virology 15:1332–1341
     [Google Scholar]
  42. Theilen G. H., Kawakami T. G., Rush T. G., Munn R. B. 1969; Replication of cat leukemia virus cell suspension cultures. Nature, London 222:589–590
     [Google Scholar]
  43. Tung J., Vittettu E. A., Fleissner E. F., Boyse E. A. 1975; Biochemical evidence linking the GiX thymocyte surface antigen to the gp 69/71 envelope glycoprotein of murine leukemia virus. Journal of Experimental Medicine 139:925–941
     [Google Scholar]
  44. Tung J., Yoshiki T., Fleissner W. 1976; A core polyprotein of murine leukemia virus on the surface of mouse leukemia cells. Cell 9:573–578
     [Google Scholar]
  45. Yoshiki T., Mellors R. C., Hardy W. D. 1973; Common cell surface antigen associated with murine and feline C-type RNA leukemia viruses. Proceedings of the National Academy of Sciences of the United States of America 70:1878–1882
     [Google Scholar]
  46. Yoshiki T., Mellors R. C., Hardy W., Fleissner E. 1974; Common cell surface antigen associated with mammalian C-type RNA viruses. Journal of Experimental Medicine 139:925–941
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-38-3-483
Loading
Deposition of Retrovirus Associated Antigens (p30 and gp70) on Cell Membranes of Feline and Murine Leukaemia Virus Infected Cells
J. Gen. Virol. 38, 483 (1978); https://doi.org/10.1099/0022-1317-38-3-483
/content/journal/jgv/10.1099/0022-1317-38-3-483
/content/journal/jgv/10.1099/0022-1317-38-3-483
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error