1887

Abstract

SUMMARY

The individual structural polypeptides of vesicular stomatitis virus have been examined by tryptic peptide analysis of S-methionine preparations labelled and I-preparations labelled . Isolates of the two classical serotypes of the virus (Indiana and New Jersey) and of a sub-type of the Indiana serotype, Brazil virus, were compared. The study showed that the major internal proteins of all three viruses gave similar maps, whereas the surface glycoproteins gave distinct maps that had very few spots in common. The map of the glycoprotein of Brazil virus, which has been shown previously to be more closely related serologically to Indiana virus than to New Jersey virus, did not show any greater similarity to the Indiana virus than to the New Jersey virus glycoprotein. On the other hand, peptide maps of the nucleoprotein and matrix protein showed Indiana and Brazil viruses to be more closely related to each other than to New Jersey virus.

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/content/journal/jgv/10.1099/0022-1317-38-2-351
1978-02-01
2024-04-20
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References

  1. Bray D., Brownlee S. M. 1973; Peptide mapping of proteins from acrylamide gels. Analytical Biochemistry 55:213–221
    [Google Scholar]
  2. Bussereau F., Cartwright B., Doel T. R., Brown F. 1975; A comparative study of the surface projections of different strains of vesicular stomatitis virus. Journal of General Virology 29:189–198
    [Google Scholar]
  3. Cartwright B., Brown F. 1972; Serological relationships between different strains of vesicular stomatitis virus. Journal of General Virology 16:391–398
    [Google Scholar]
  4. Cleveland D. W., FIscher S. G., Kirschner M. W., Laemmli C. K. 1977; Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. Journal of Biological Chemistry 252:1102–1106
    [Google Scholar]
  5. Crestfield A. M., Moore S., Stein W. H. 1963; The preparation and enzymatic hydrolysis of reduced and S-carboxylmethylated proteins. Journal of Biological Chemistry 238:622–628
    [Google Scholar]
  6. Davison P. F. 1976; An appraisal of radioiodination methods for peptide mapping. Analytical Biochemistry 75:129–141
    [Google Scholar]
  7. Federer K. E., Burrows R., Brooksby J. B. 1967; Vesicular stomatitis virus - the relationship between some strains of the Indiana serotype. Research in Veterinary Science 8:103–117
    [Google Scholar]
  8. Harris T. J. R., Doel T. R., Brown F. 1977; Molecular aspects of the antigenic variation of swine vesicular disease and Coxsackie B5 viruses. Journal of General Virology 35:299–315
    [Google Scholar]
  9. Rang C. Y., Prevec L. 1970; Proteins of vesicular stomatitis virus. II. Immunological comparisons of viral antigens. Journal of Virology 6:20–27
    [Google Scholar]
  10. Laver W. G., Downie J. C. 1976; Influenza virus recombination. I. Matrix protein markers and segregation during mixed infection. Virology 70:105–117
    [Google Scholar]
  11. Repik P., Flamand A., Clark H. F., Obijeski J. F., Roy P., Bishop D. H. L. 1974; Detection of homologous RNA sequences among six rhabdovirus genomes. Journal of Virology 13:250–252
    [Google Scholar]
  12. Wagner R. R., Prevec L., Brown F., Summers D. F., Sokol F., Macleod R. 1972; Classification of rhabdovirus proteins: a proposal. Journal of Virology io:1228–1230
    [Google Scholar]
  13. Weber K., Osborn M. 1969; The reliability of molecular weight determinations by dodecyl sulphate polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
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