1887

Abstract

SUMMARY

Guinea pigs immunized with hepatitis B surface antigen (HBsAg), types and , and with two major polypeptides derived from HBsAg/ developed cell-mediated immunity as determined by the macrophage migration inhibition assay. Peritoneal exudate cells from animals immunized with a 22000- or a 25000-mol. wt. polypeptide derived from HBsAg/ showed significant migration inhibition after challenge with either polypeptide or with purified HBsAg. Significant inhibition of macrophage migration was not observed when polypeptide-sensitized cells were challenged with normal human serum or with normal human liver extract. Similarly, a cell-mediated immune response was not observed in peritoneal exudate cells from animals sensitized to normal human serum or normal human liver extract which were challenged with either of the polypeptides. The humoral immune response to either of the polypeptides, as measured by radioimmunoassay, was substantially lower than that observed in animals immunized with intact particles. This apparent difference between cellular and humoral responses suggests that the macrophage migration assay is a sensitive indicator of the immunogenicity of the smaller mol. wt. HBsAg-derived polypeptides in guinea pigs.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-38-2-339
1978-02-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/38/2/JV0380020339.html?itemId=/content/journal/jgv/10.1099/0022-1317-38-2-339&mimeType=html&fmt=ahah

References

  1. Allison A. C. 1967; Cell-mediated immune responses to virus infections and virus-induced tumours. British Medical Bulletin 23:60–65
    [Google Scholar]
  2. Brown R. K. 1962; Studies on the antigenic structure of ribonuclease. III. Inhibition by peptides of antibody to performic acid-oxidized ribonuclease. Journal of Biological Chemistry 237:1162–1167
    [Google Scholar]
  3. Brown R. K., Delaney R., Levine L., Van Vunases H. 1959; Studies on the antigenic structure of ribonuclease. I. General role of hydrogen and disulfide bonds. Journal of Biological Chemistry 234:2043–2049
    [Google Scholar]
  4. Brown R. K., Trzpis M. A., Sela M., Aufinsen C. B. 1963; Studies on the antigenic structure of ribonuclease. IV. Polyalamyl ribonuclease. Journal of Biological Chemistry 238:3876–3883
    [Google Scholar]
  5. Cabral G. A., Chairez R., Marciano-Cabral F., Suarez M., Dreesman G. R., Melnick J. L., Hollinger F. B. 1975; Cell-mediated immunity in guinea pigs to subunits derived from hepatitis B surface antigen. Infection and Immunity 12:564–570
    [Google Scholar]
  6. Chairez R., Steiner S., Melnick J. L., Dreesman G. R. 1973; Glycoproteins associated with hepatitis B antigen. Intervirology 1:224–228
    [Google Scholar]
  7. Chairez R., Hollinger F. B., Melnick J. L., Dreesman G. R. 1974; Biophysical properties of purified morphologic forms of hepatitis B antigen. Intervirology 3:129–140
    [Google Scholar]
  8. Chairez R., Hollinger F. B., Brunschwig J. P., Dreesman G. R. 1975; Comparative biophysical studies of hepatitis B antigen, subtypes adw and ayw. Journal of Virology 15:182–190
    [Google Scholar]
  9. David J. R., David R. 1972; Cellular hypersensitivity and immunity. Inhibition of macrophage migration and the lymphocyte mediators. Progress in Allergy 16:300–499
    [Google Scholar]
  10. Dreesman G. R., Hollinger F. B., Mccombs R. M., Melnick J. L. 1972; Production of potent anti-Australia antigen sera of high specificity and sensitivity in goats. Infection and Immunity 5:213–221
    [Google Scholar]
  11. Dreesman G. R., Chairez R., Suarez M., Hollinger F. B., Courtney R. J., Melnick J. L. 1975a; Production of antibody to individual polypeptides derived from purified hepatitis B surface antigen. Journal of Virology 16:508–515
    [Google Scholar]
  12. Dreesman G. R., Hollinger F. B., Melnick J. L. 1975b; Biophysical and biochemical properties of purified preparations of hepatitis B surface antigen (HBsAg). American Journal of Medical Science 270:123–129
    [Google Scholar]
  13. Fields H. A., Hollinger F. B., Desmyter J., Melnick J. L., Dreesman G. R. 1977; Biochemical and biophysical properties of hepatitis B core particles derived from Dane particles and infected hepatocytes. Intervirology 8:336–350
    [Google Scholar]
  14. Figenschau K. J., Ulstrup J. C. 1974; Staphylococcal radioimmunoassay for hepatitis B antigen and antibody. Acta Pathologica et Microbiologica Scandinavica B 82:422–428
    [Google Scholar]
  15. Gerwing J., Thompson K. 1968; Studies on the antigenic properties of egg-white lysozyme. I. Isolation and characterization of a tryptic peptide from reduced and alkylated lysozyme exhibiting haptenic activity. Biochemistry 7:3888–3892
    [Google Scholar]
  16. Glasgow L. A. 1970; Cellular immunity in host resistance to viral infections. Archives of Internal Medicine 126:125–134
    [Google Scholar]
  17. Gold J. W. M., Shih J. W., Purcell R. H., Gerin J. L. 1976; Characterization of antibodies to the structural polypeptides of HBsAg: evidence for subtype-specific determinants. Journal of Immunology 117:1404–1406
    [Google Scholar]
  18. Hollinger F. B. 1974; Hepatitis viruses. In Manual of Clinical Microbiology 2nd edition, pp 819–833 Edited by Lennette E. H., Spaulding E. H., Truant J. P. Washington: American Society for Microbiology;
    [Google Scholar]
  19. Lamelin J. P. 1971; Inhibition of macrophage migration. In Cell-Mediated Immunity in vitro Correlates pp 75–98 Edited by Revillard J. P. Basel: S. Karger;
    [Google Scholar]
  20. Lee W. M., Reed W. D., Mitchell C. G., Galbraith R. M., Eddleston A. L. W. F., Zuckerman A., Williams R. J. 1975; Cellular and humoral immunity to hepatitis B surface antigen in active chronic hepatitis. British Medical Journal 1:705–708
    [Google Scholar]
  21. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  22. Millman I., Hutanen H., Merino F., Manfred E. B., Blumberg B. S. 1971; Australia antigen: physical and chemical properties. Research Communications in Chemical Pathology and Pharmacology 2:667–686
    [Google Scholar]
  23. Neurath A. R., Prince A. M., Lippin A. 1974; Hepatitis B antigen: antigen sites related to human serum proteins revealed by affinity chromatography. Proceedings of the National Academy of Sciences of the United States of America 71:2663–2667
    [Google Scholar]
  24. Parish C. R. 1971; Immune response to chemically modified flagellin. II. Evidence for a fundamental relationship between humoral and cell-mediated immunity. Journal of Experimental Medicine 134:21–47
    [Google Scholar]
  25. Scheidegger J. J. 1955; Une micromethode de l’immuno-electrophorese. International Archives of Allergy 7:103–110
    [Google Scholar]
  26. Thompson K., Harris M., Benjamini E., Mitchell G., Noble M. 1972; Cellular and humoral immunity: a distinction in antigenic recognition. Nature New Biology 238:20–21
    [Google Scholar]
  27. Tompkins W. A. F., Adams C., Rawls W. E. 1970a; An in vitro measure of cellular immunity to fibroma virus. Journal of Immunology 104:502–510
    [Google Scholar]
  28. Tompkins W. A. F., Zarling J. M., Rawls W. E. 1970b; In vitro assessment of cellular immunity to vaccinia virus: contribution of lymphocytes and macrophages. Infection and Immunity 2:783–790
    [Google Scholar]
  29. Young J. D., Leung C. Y. 1970; Immunochemical studies on lysozyme and carboxymethylated lysozyme. Biochemistry 9:2755–2762
    [Google Scholar]
  30. Zuckerman A. J. 1975; Hepatitis vaccine: a note of caution. Nature, London 255:104–105
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-38-2-339
Loading
/content/journal/jgv/10.1099/0022-1317-38-2-339
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error