RT Journal Article SR Electronic(1) A1 Takeda, Hisao A1 Oya, Akira A1 Hashimoto, Katsuyuki A1 Yasuda, Tomoyoshi A1 Yamada, Masa-AtsuYR 1978 T1 Association of Virus Specific Replicative Ribonucleic Acid with Nuclear Membrane in Chick Embryo Cells Infected with Japanese Encephalitis Virus JF Journal of General Virology, VO 38 IS 2 SP 281 OP 291 DO https://doi.org/10.1099/0022-1317-38-2-281 PB Microbiology Society, SN 1465-2099, AB SUMMARY Using high resolution electron microscopic autoradiography and velocity sedimentation, RNA synthesis was examined in chick embryo cells infected with Japanese encephalitis virus (JEV). RNA was labelled with 3H-uridine for 5 min or 10 min at 15 h after infection in the presence of actinomycin D and d-glucosamine. Microautoradiography showed significant numbers of silver grains on the nuclear membranes of 5 min pulse-labelled thin cell sections. The RNA species in membrane fractions obtained from the nucleus and cytoplasm of the infected cells were analysed by sucrose density gradient sedimentation. Radioactive 23S replicative form RNA and 8-12S RNA were obtained from the outer membrane fractions of the nuclear envelope. Labelled 42S virus RNA was obtained from the fractions containing large vesicle membranes and plasma membranes. These results suggest that JEV-RNA synthesis is initiated in the perinuclear region in close association with the outer membranes of the nuclear envelope., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-38-2-281