Some morphological, biological, immunological and biochemical characterizations of a virus, rat helper virus pseudotype Kirsten sarcoma virus, KiMSV-(RHHV), have been presented here. KiMSV(RHHV) has a type C virus ultrastructure. It is strictly rat tropic and is able to transform rat cells in vitro. The possibility of its being a xenotropic mouse virus has been carefully ruled out by exhaustive analyses of host range and immunological studies. Antigenically KiMSV (RHHV) demonstrates cross reactivity with an antiserum specific against rat leukaemia virus, no cross reactivity with antiserum against Moloney leukaemia virus, and only minor cross reactivity with antiserum against cat leukaemia virus. Analysis of virus proteins and glycoproteins by equilibrium acrylamide gradient gel electrophoresis showed that the virus complex possesses both a gp70 fraction and a p30 fraction. KiMSV(RHHV) sediments isopycnically in a linear sucrose gradient at 1.145 to 1.155 g/ml and possesses RNA and reverse transcriptase activity.
BonnerW. M.,
LaskeyR. A.1974; A film detection method for tritium-labelled-proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
CharmanH. P.,
WhiteM. H.,
RahmanR.,
GildenR. V.1976; Species and interspecies radioimmunoassays for rat type C virus P30: interviral comparisons and assay of human tumor extracts. Journal of Virology 17:51–59
Duc-NguyenH.,
RosenblumE. N.,
ZeigelR. F.1966; Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus. Journal of Bacteriology 92:1133–1140
HartleyJ. W.,
RoweW. P.1975; Clonal cell lines from a feral mouse embryo which lack host-range restrictions for murine leukemia viruses. Virology 65:128–134
KlementV.,
NicolsonM. O.,
HuebnerR. J.1971; Rescue of the genome of focus forming virus from rat non-productive lines by 5′-bromodeoxyuridine. Nature New Biology 234:12–14
LaskeyR. A.,
MillsA. D.1975; Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56:335–341
MaizelJ. V.Jun1971; Polyacrylamide gel electrophoresis of viral proteins. In Methods in Virology vol 5 pp 179–246 Edited by
MaramoroschK.,
KoprowskiH.
New York: Academic Press;
OrensteinJ. M.,
WeinsteinI. B.1973; Filamentous forms of enveloped A particles in cell cultures from chemically induced rat hepatomas. Cancer Research 33:1998–2004
ParksW. P.,
HowkR. S.,
AnisowiczA.,
ScolnickE. M.1976; Deletion mapping of Moloney type C virus: polypeptide and nucleic acid expression in different transforming virus isolates. Journal of Virology 18:491–503
ScolnickE. M.,
MaryakJ. M.,
ParksW. P.1974; Levels of rat cellular RNA homologous to either Kirsten sarcoma virus or rat type-C virus in cell lines derived from Osborn-Mendel rats. Journal of Virology 14:1435–1444
ThompsonE. B.,
TomkinsG. M.,
CurranJ. F.1966; Induction of tyrosine-ketoglutarate transaminase by steroid hormone in a newly established tissue culture cell line. Proceedings of the National Academy of Sciences of the United States of America 56:296–302
TingR. C.1968; Biological and serological properties of viral particles from a non-producer rat neoplasm induced by murine sarcoma virus (Moloney). Journal of Virology 2:865–868
ValentineA. F.,
BaderJ. P.1968; Production of virus by mammalian cells transformed by Rous sarcoma and murine sarcoma viruses. Journal of Virology 2:224–237
WeinsteinL. B.,
GebertR.,
StadlerU. C.,
OrensteinJ. M.,
AxelR.1972; Type-C virus from cell cultures of chemically induced rat hepatomas. Science 178:1098–1100
YangS. S.,
ChanC.,
ThompsonE. B.1976; Rescue of a rat tropic rat hepatoma virus pseudotype Kirsten sarcoma virus by co-cultivation of hepatoma tissue culture cells with K-NRK cells. Journal of General Virology 31:239–243
YangS. S.,
HerreraF. M.,
SmithR. G.,
ReitzM. S.,
LanciniG.,
TingR. C.,
GalloR. C.1972; Rifamycin antibiotics: inhibitors of Rauscher murine leukemia virus reverse transcriptase and of purified DNA polymerase from human normal and leukemia lymphoblasts. Journal of the National Cancer Institute 49:7–25